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May Grunwald
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GIEMSA MAY GRUNWALD TECHNIQUE for cytology.
www.cellpath.comThe May-Grunwald stain combines the effect of acidic eosin and alkaline methylene blue to stain cell compo-nents orange-pink, or blue. Whereas the Giemsa stain utilises the azure dye to stain the azurophilic cellular components purple. Staining must be performed at the correct pH for accurate results. Any change will lead to wrong staining reac-
Giemsa - Sheehan's Modified May-Grunwald - University of …
webpath.med.utah.edu2. Methanol, three changes. 3. Place slide on staining rack, cover with Wright stain, 5 minutes. 4. Do not drain off stain, add an equal amount of distilled water until a metallic sheen appears. Leave for 5 minutes. 5. Place slides directly into the Giemsa solution, for 45 minutes, room temperature.