Example: air traffic controller
Multisite Gateway Overview
Found 2 free book(s)pENTR™ Directional TOPO® Cloning Kits
assets.thermofisher.comSystem overview . The pENTR ... Cloning") to directionally clone a blunt-end PCR product into a vector for entry into the Gateway ® System or the MultiSite Gateway System. Blunt-end PCR products clone directionally at greater than 90% efficiency, with no ligase, post-PCR procedures, or restriction enzymes required. ...
MULTISITE GATEWAY OVERVIEW - University of Guelph
www.uoguelph.caMULTISITE GATEWAY OVERVIEW Creating entry clone: Amplify 2 DNA fragments using primers with attB sites on them (see next page). Recombination of attB and attP produces attL site.