Troubleshooting Guide For Nucleic Acid
Found 6 free book(s)
Good Laboratory Pipetting Guide - Pipette Calibration
pipettecalibration.netTroubleshooting 23 Good Laboratory Pipetting Guide. www ... The following terms are used throughout this guide. Aspirate – to draw the liquid up into the pipette tip Dispense – to discharge the liquid from the tip ... Protein and nucleic acid solutions, glycerol, Tween …
QPCR Optimization & Troubleshooting Guide
assets.thermofisher.comToo much nucleic acid in 'high copy number' assays Use less than 500ng of template in each QPCR or QRT-PCR reaction. Too little nucleic acid present in 'low copy number' assays Increase the amount of template or increase PCR reaction efficiency by optimizing thermal protocol / re-designing primers. Annealing temperature too low
Agilent 2100 Bioanalyzer System 2100 Expert Software …
www.agilent.comUser's Guide. 2100 Expert Software User Guide Notices ... Principles of Nucleic Acid and Protein Analysis on a Chip 53 Preparing and Running an Electrophoretic Method or Assay 57 ... Example: Refer to the Agilent 2100 Bioanalyzer System Troubleshooting and …
Droplet Digital Applications Guide - Bio-Rad
www.bio-rad.comDroplet Digital PCR Applications Guide | 1 1 oplet DigitalDr ™ PCR Introduction Droplet Digital polymerase chain reaction (ddPCR™) was developed to provide high-precision, absolute quantification of nucleic acid target sequences with wide-ranging applications for both research and clinical diagnostic applications. ddPCR measures
Nucleic Acid - Thermo Fisher Scientific
tools.thermofisher.com2. Open the Nucleic Acid application. Load an aliquot of the blanking buffer onto the lower measurement pedestal and lower the sampling arm. Tip: The blank solution should be the same pH and of a similar ionic strength as the sample solution. For nucleic acid samples, blank buffers are generally dH 2 O or TE. Blanking with water
Instruction Manual and Application Guide
www.bio-rad.com2 Technical Support: 1-800-4BIORAD • 1-800-424-6723 • www.bio-rad.com 1.2 Gel Formulations Mini-PROTEAN precast gels are composed of polyacrylamide with a bisacrylamide crosslinker, and they are available in a range of formulations (Table 1.1) and in a selection of single percentages and gradients.