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SIZE EXCLUSION CHROMATOGRAPHY FOR BIOMOLECULE …
www.agilent.comColumn Selection Column size SEC columns are usually much larger than those used for other types of chromatography and are operated at comparatively low flow rates or slow linear velocities. The standard column dimensions for SEC are 7.8 x 300 mm, operated at 1.0 mL/min, compared to a reversed-phase column that is likely to be 2.1 or 4.6 x 150 ...