Search results with tag "Microbiology biol 275"
ENUMERATION OF MICROORGANISMS I. OBJECTIVES II. …
www.sas.upenn.eduMicrobiology BIOL 275 Dr. Eby Bassiri ebassiri@sas.upenn.edu 3 Viable Count The most common procedure for the enumeration of bacteria is the viable plate count. In this method, serial dilutions of a sample containing viable microorganisms are plated onto a suitable growth medium. The suspension is either spread onto the surface
PURE CULTURE TECHNIQUES I. OBJECTIVES
www.sas.upenn.eduMicrobiology BIOL 275 Dr. Eby Bassiri ebassiri@sas.upenn.edu 3 fastidious. Fastidious organisms are those which have specific nutritional requirements for growth. • Sheep Blood Agar (SBA): This is a medium with TSA, SNA or BHI as a base and 5% sheep blood. Blood is incorporated into the medium to provide growth factors
Microbiology BIOL 275 DILUTIONS - School of Arts & Sciences
www.sas.upenn.edudiscussed here are used in producing a series of solutions having equal increments of dilution. Note that "serial dilution" is a special case of "series of dilutions". Example: A serum sample is diluted twice with buffer. A series of five dilutions is made of this first