Search results with tag "Protocol 3"

Protocol 3: Immunofluorescence on Frozen Sections

2. Wash 1 – 2 times in PBS and transfer to a glass vial on ice. 3. oCover with 4% PFA and incubate at 4 C for 30 minutes to several hours depending on the size of the tissue. 4. Wash with PBS, 1X fast followed by 2 – 3 times at 4. o. C over the co urse of several hours with slow agitation (such as on a nutator). 5.

  Section, Protocol, Transfer, Frozen, Immunofluorescence, Protocol 3, Immunofluorescence on frozen sections