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Bacterial Culture Techniques - DNALC::Protocols
labprotocols.dnalc.orgof overnight culture (the inoculum) is added to 100 volumes of fresh LB broth in an Erlenmeyer flask. To provide good aeration for bacterial growth, the ... We prefer LB (Luria-Bertani) agar, but almost any rich nutrient agar can be used for plating cells. Presterilized, ready-to-pour agar is a great convenience. It needs