Search results with tag "Pcr protocol"

QUANTITATIVE RT-PCR PROTOCOL (SYBR Green I)

Apr 01, 2007 · Quantitative RT-PCR Protocol (SYBR Green I) 5 (3) Running 3% agarose gel after qRT-PCR (4) Analyze qRT-PCR data: Select those primers with one band in the gel picture and one peak in the dissociation curve for further experiments. At the same time, have a look at the Ct value to have a rough idea about how much RNA/cDNA is enough for the further

  Protocol, Sybr, Pcr protocol