Search results with tag "Superscript iii first strand synthesis"

SuperScript III First-Strand Synthesis System for RT-PCR

cDNA synthesis from high-GC content transcripts, page 3. Increase the temperature of first-strand reaction (up to 55°C). Use PCR primers closer to the 3´ terminus of the target cDNA. Too little first-strand product was used in PCR . Use up to 10 µL of the first-strand reaction. GSP was used for first-strand synthesis

  First, Synthesis, Strand, Superscript, Superscript iii first strand synthesis