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Real-Time PCR Vs. Traditional PCR - Gene-Quantification
www.gene-quantification.dePCR amplification at the final phase or end-point of the PCR reaction. For Reference Only Page 3 of 15 Limitations of End-Point PCR Agarose gel results are obtained from the end point of the reaction. ... Here the polymerase is adding bases to a growing chain of DNA. Subsequently, the polymerase is removing DNA that is downstream, impeding its ...
Polymerase Chain Reaction (PCR) - University of Toledo
www.eeescience.utoledo.eduPolymerase Chain Reaction, 12/2004 3 • A control reaction, omitting template DNA, should always be performed, to confirm the absence of contamination. The protocol in brief You will perform a PCR reaction on you DNA sample to generate multiple copies of a portion of the 16S rRNA gene. The first step involves setting up a master