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A theoretical and practical guide for spectrophotometric ...
assets.thermofisher.comTECHNICAL NOTE CNanoDrop One/One and Multiskan Sky spectrophotometers A theoretical and practical guide for spectrophotometric determination of protein concentrations at 280 nm of a given protein is not strictly dependent on amino acid content but also on buffer type, ionic strength, and the pH of the protein solution. Different preparations of the