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4. Designing and validating primers - Research at St ...

4. Designing and validating primers - Research at St ...

stmichaelshospitalresearch.ca

4. Designing and validating primers DESIGN: When designing primers, consideration should be given to the area of the target gene being amplified (target sequence) and the thermodynamic properties of the primers themselves. All of these properties factor into the efficiency and specificity of target gene amplification. Forward and reverse

  Research, Designing, Primer, Designing primers

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