Search results with tag "Glycan analysis"
Guide to Glycan Analysis - Thermo Fisher Scientific
tools.thermofisher.comHPAE–PAD is a well-established glycan analysis method that separates carbohydrates with specific interactions between the hydroxyl and carboxyl groups of glycans, which are charged at high pH, and the stationary phase. This separation resolves glycans based on charge, size, composition, isomers, and
N-Glycan Analysis of Monoclonal Antibodies - IPT Online
iptonline.comThe dried glycan samples were labelled with 2-AB using the GlycoProfi l 2-AB Labeling Kit according to the preparation protocol from Sigma-Aldrich for three hours at 65°C. After the labelling procedure, the samples were purified using the GlycoProfi l Glycan Clean-up Cartridges from Sigma-Aldrich. After the HILIC clean-up procedure, the
N-Glycan Analysis of Monoclonal Antibodies
www.iptonline.com22 Innovations in Pharmaceutical Technology Issue 42 Laboratory Technology Keywords N-glycan analysis Protein glycosylation Monoclonal antibodies (mAbs) Ultra high performance liquid chromatography (UHPLC)
Troubleshooting CE-SDS: baseline disturbances, peak area ...
cdn.ymaws.comPAGE, IEF), but in novel applications as well (e.g. glycan analysis, preparative charge-based fractionation). Performing gel electrophoresis in a capillary has the added advantage of direct quantitation without the need for staining/destaining. And because the capillary is rather rugged,