Search results with tag "Ffpe"
SureSelect XT HS2 DNA System - Agilent
www.agilent.comPool samples for multiplexed sequencing 68 Step 5. Prepare sequencing samples 70 Step 6. Do the sequencing run and analyze the data 72 Sequence analysis resources 77 6 Appendix: Using FFPE-derived DNA Samples 79 Protocol modifications for FFPE Samples 80 Methods for FFPE Sample Qualification 80 Sequencing Output Recommendations for FFPE Samples 81
Quick-DNA™ Miniprep Plus Kit - Zymo Research
files.zymoresearch.comApr 16, 2021 · • For solid tissue samples stored in DNA/RNA Shield™, see Appendix B (pg. 11). • For hair and feather samples, see Appendix D (pg. 13). • For FFPE samples, see the Quick-DNA™ FFPE Kit (D3067) for specialized FFPE DNA purification. See Appendix E (pg. 14) for an adapted protocol using the Quick-DNA™ Miniprep Plus Kit. Notes:
FoundationOne CDx Intended Use Table 1 - Foundation …
info.foundationmedicine.comformalin-fixed, paraffin-embedded (FFPE) tumor samples. The assay employs a single DNA extraction method from routine FFPE biopsy or surgical resection specimens; 50-1000 ng of DNA will undergo whole-genome shotgun library construction and hybridization-based capture of all coding exons from 309 cancer-related genes,
VENTANA MMR IHC Panel - Roche Diagnostics
diagnostics.roche.combetween the loss of DNA mismatch repair (MMR) function and cancer.5 Loss of any MMR protein (MLH1, PMS2, ... This guide is intended to aid pathologists in the clinical evaluation of formalin-fixed, paraffin-embedded (FFPE) ... control antibody is used to evaluate the presence of background in test samples and establish a staining intensity
Applications for DNA, RNA, and Protein Analysis
www.agilent.comQuality control of single cell DNA samples 48 Genomic DNA Optimization of gDNA Extraction from FFPE tissue 49 Equivalence of the 4200 TapeStation and 2200 TapeStation systems 50 Quantitation of isolated genomic DNA (gDNA) samples 51 Quantity and integrity analysis of genomic DNA for next generation sequencing 52 Integrity analysis of genomic ...
CPT Proprietary Laboratory Analyses Codes Long Descriptors
www.ama-assn.orgformalin-fixed paraffin-embedded (FFPE) tumor tissue, algorithmic interpretation reported as a recurrence risk score October 1, 2021 January 1, 2022 ® 2023 MindX Blood Test™ - Memory/Alzheimer's, MindX Sciences™ Laboratory, MindX Sciences™ Inc ⚫0289U Neurology (Alzheimer disease), mRNA, gene expression profiling by RNA
Whole Exome Sequencing and Analysis
www.nisc.nih.govOct 22, 2018 · DNA from FFPE is always damaged to some degree by the harsh chemical treatments used to preserve the tissue. The degree of damage is influenced by a number of factors, the protocol used in fixing the tissue, the length of time the tissue was stored, and the DNA extraction protocol used. Some damage can be repaired, but not all.
DNA Reference Guide - Thermo Fisher Scientific
assets.thermofisher.comtissue, formalin-fixed, paraffin-embedded (FFPE) samples, forensic samples, buccal samples) • Process up to 96 individual sample inputs of up to 25 mg in 35 minutes • Isolate up to 10 µg of gDNA from tissue using silica filter plate technology • …
RT-qPCR guidelines - University of California, Davis
pcrlab.vetmed.ucdavis.eduE = essential, D = desirable, FFPE = formalin-fixed paraffin embedded Description E Volume/mass of sample processed D Microdissection or macrodissection E Processing procedure E If frozen, how and how quickly? E If fixed, with what and how quickly? E
Techniques for epigenetic analysis - Harvard University
cdn1.sph.harvard.edu• FFPE are not easy to work with – For some disease we can get at least close • Bladder cancer →Cells in urine sediments • Leukemias →White blood cells (benzene effects in Bollati et al Cancer Res 2007)
SureSelect Target Enrichment System for the Illumina Platform
www.agilent.comthe prepared library DNA. 5 Indexing and Sample Processing for Multiplexed Sequencing This chapter describes the steps to amplify, purify, and assess quality and quantity of the sample libraries. Samples are pooled by mass prior to sequencing. 6 Appendix: Using FFPE-derived DNA Samples This chapter contains recommended protocol modifications
NEBNext FFPE DNA Repair Mix - Euroclone group
www.euroclonegroup.itSequenziamento Quanti˜cazione e QC Bio˚uidi ed Esosomi - Tecnologia Zymo Research per l’isolamento di DNA cell-free (cfDNA) circolante. Isolamento e caratterizzazione di esosomi da