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Chapter 10 - MICROBIOLOGICAL ANALYSES
www.who.int• For samples of drinking-water, one tube with 50 ml of sample and five tubes with 10 ml of sample are inoculated and incubated. The results are compared with the values such as those given in Table 10.7 (see later) to obtain the MPN. 10.3.1 Culture media and buffered dilution water Each part of the test requires a different type of medium.
Buffered Dilution Water Composition**
himedialabs.comR094 Butterfield's Phosphate Buffered Dilution Water ,pH 7.2 is used for preparation of microbiological dilution blanks. This buffer is used rather than unbuffered water in order to standardized this potential variable due to the wide variation in the pH