Search results with tag "293t"
CULTURE OF HEK 293/293T C - Bowdish Lab
www.bowdish.caCULTURE OF HEK 293/293T CELLS Charles Yin Last Updated: 11 August 2014 Bowdish Lab, McMaster University Hamilton, ON, Canada www.bowdish.ca BACKGROUND - HEK 293/293T cells are derived from human embryonic kidney transformed with adenovirus 5 DNA
Titering of virus in a 96-well plate format
www.mdanderson.orgDay 1, prepare HEK-293 T cells 1. Digest 293T cells (at log growth phase) and seed the cells into 6-well plate with 5x105 cells/well; 2. Incubate the cells at 37 oC/5% CO 2 overnight (the cells will become about 50-60% confluence on next day); Note: Prepare an extra plate for cell counting on next day; Day 2, prepare virus infection 1.
Tet発現誘導システムガイド - takara-bio.co.jp
www.takara-bio.co.jpP TREG3G 目的遺伝子 PGK Tet-On 3G遺伝子 P TREG3G 目的遺伝子 PGK Tet-On 3G遺伝子 Hygromycin or Puromycin selection pTetOne-GOl Hygr r/Pur-Dox +Dox Expression OFFE xpression ON 293T Cells Doxycycline concentratio n（ng /ml） Luci