Search results with tag "Sybr green"
QuantStudio 3 and QuantStudio 5 Real-Time PCR Systems
tools.thermofisher.comoptimized master mixes, enable outstanding specificity and sensitivity. Applied Biosystems™ SYBR™ Green chemistry is an economical alternative for target identification or initial screening assays. The QuantStudio 3 system has 4 filters calibrated for FAM™/SYBR Green, VIC™/JOE™, NED™/ TAMRA™, and ROX™ dyes. The QuantStudio 5 ...
iQ SYBR Green Supermix - Bio-Rad
www.bio-rad.comiQ™ SYBR ® Green Supermix Catalog # Supermix Volume Kit Size 170-8880 2.5 ml (2 x 1.25 ml vials) 100 x 50 µl reactions 170-8882 12.5 ml …
4. Designing and validating primers
stmichaelshospitalresearch.caPCR reactions to determine the optimal concentration of primers to use for amplification (usually ... SYBR Green Mix 2x 5.00 μl 21 105 μl 1x Primer mix ... (22.174, 22.471, 24.349) were assessed and the outlier (24.349) was omitted from the analysis. For standard curves in which a 1:10 dilution is performed, the