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Tips for practical HPLC analysis
www.shimadzu.eu.comHPLC-type acetonitrile has the lowest absorbance (particularly for short wavelengths). Using an organic solvent with lower absorbance as the mobile phase results in less noise in UV detection, and so HPLC-type acetonitrile is suitable for high-sensitivity analysis in the UV short-wavelength range. Also, using HPLC-type acetonitrile