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Search results with tag "Ligation cloning"
USE OF BACILLUS SUBTILIS sacB (LEVANSUCRASE) …
www.uwstructuralgenomics.orgAbstract Flexi®Vector system is a directional restriction/ligation cloning method developed by Promega (Madison, WI). In the plasmids used for this cloning method, a lethal barnase gene insert is flanked by two rare-cutting