Search results with tag "Reverse transcriptases"

Incorporation by MMLV-Derived Reverse Transcriptases Base ...

linnarssonlab.org

Base Preferences in Non-Templated Nucleotide Incorporation by MMLV-Derived Reverse Transcriptases Pawel Zajac¤a, Saiful Islam, Hannah Hochgerner¤b, Peter Lönnerberg, Sten Linnarsson* Laboratory for Molecular Neurobiology, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden

Reserve, Preference, Nucleotide, Templated, Preferences in non templated nucleotide, Reverse transcriptases, Transcriptases

SuperScript III First-Strand Synthesis System for RT-PCR

tools.thermofisher.com

Reverse Transcriptase is a version of M-MLV RT that has been en gineered to reduce RNase H activity and provide increased therma l stability. The enzyme is used to synthesize cDNA at a temperature range of 42–55°C, providing increased specificity, higher yields of cDNA, and more full-length product than other reverse transcriptases.

First, Reserve, Strand, Reverse transcriptases, Transcriptases, Superscript, Superscript iii first strand

BAB I - UB

www.muhaiminrifai.lecture.ub.ac.id

enzim reverse transcriptases yang berfungsi membentuk blue-print dari molekul RNA membentuk cDNA (DNA komplementer). Enzim ini dibuat dari virus RNA yang mengubah genom RNA virus menjadi DNA ketika virus menginfeksi inangnya. Enzim ini biasa dipakai ketika bertemu dengan gen eukariotik yang biasanya terpisah-pisah

Reserve, Reverse transcriptases, Transcriptases, Bab i

Design and optimization of SYBR Green assays

assets.thermofisher.com

the reverse transcriptases. RNA quantity RNA quantity 60 40 20 0 RAS C t C t GAPDH RAS GAPDH 60 40 20 0. SYBR Green assays, step 1: bioinformatics 1. Obtain the sequence for the gene of interest, and select an exon-to-exon spanning region ~200 bp in length. 2. Use a tool such as SNPmasker

Reserve, Reverse transcriptases, Transcriptases

PCR solutions brochure - Thermo Fisher Scientific