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Search results with tag "Lentivirus"
Lentivirus infection and general screening plan Adherent cells
weissmanlab.ucsf.eduLentivirus infection and general screening plan—Adherent cells Prepping for screening -Ensure you have enough media and plates for screen -Test small scale (~6-well plate size) transfection and infection …
Lentivirus Protocol - Perelman School of Medicine
www.med.upenn.eduO (autoclaved and .22 filter sterilized) 20 μg of DNA per transfection -3 μg Rev, 5 μg gp, 1.5 μg VSV G make up 9.5 μg, the rest is transfer vector . 2M CaCl. 2 2xHBS . 50mM Chloroquine . 1)Check the confluency of the cells. 80-90%. 2)Prepare DNA for transfection, ALL REAGENTS AT ROOM TEMPERATURE