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A Guide to Polyacrylamide Gel Electrophoresis and Detection
www.bio-rad.comSample Quantitation (RC DC™ Protein Assay) 56 Standard Assay Protocol (5 ml) 56 Microfuge Tube Assay Protocol (1.5 ml) 56 Handcasting Polyacrylamide Gels 57 Single-Percentage Gels 57 Pour the Resolving Gel 58 Pour the Stacking Gel 58 Gradient Gels 59 Performing Electrophoresis 60 General Protocols: SDS-PAGE 60 Total Protein Staining 62
DC Protein Assay Instruction Manual - Bio-Rad Laboratories
www.bio-rad.comSection 1 Introduction and Principle The Bio-Rad DC Protein Assay is a colorimetric assay for protein concen-tration following detergent solubilization.