Biosupplies Australia Pty Ltd

1 MONOCLONAL ANTIBODY TO. (1 3,1 4)- -GLUCAN. (MOUSE IgG, KAPPA LIGHT). FOR RESEARCH PURPOSES ONLY. Cat. No. 400-3. SPECIFICITY USE. This antibody recognizes linear (1 3,1 4)- - Reconstitute in 150 L of 120mM NaCl. When oligosaccharide segments in (1 3,1 4)- - reconstituted, the antibody concentration is glucans. It has no cross-reactivity with (1 3)- 1mg/mL in 10mM phosphate buffer (pH ), -glucans. % sodium chloride. This antibody can be used with second stage, gold-labelled rabbit anti-mouse antibody for STORAGE. immunohistochemical studies This freeze-dried product has been shipped at ambient temperature, however it should be TITRE stored for extended periods at less than 4 C, with desiccant. Indirect ELISA titre against the following - glucan-BSA conjugates were: Once reconstituted, store at 4 C short term, or BSA-(1 3,1 4)- -glucans 10ng/mL at -20 C in aliquots with desiccant for long BSA-cellopentaose 200ng/mL term.

2 Do not freeze again after thawing. Once BSA-laminarin >100,000ng/mL thawed, store at 4 C. Antibody binding to the BSA-(1 3,1 4)- - glucan conjugate was inhibited (50%) by REFERENCES. G3G4G4G3G4G4GR ( ), G3G4GR (10-2M), Meikle, Hoogenraad, Bonig, I. Clarke, G4G4GR (10-2M) and G3G3GR (no inhibition). & Stone, (1994). The Plant Journal 51: 1-9. Biosupplies Australia 62 006 209 034. Pty Ltd Box 187, La Trobe Uni, Bundoora, Victoria 3083, Australia .