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1. Plasmid structure 2. Plasmid replication and copy ...
1. RepA mRNA is made from Prep until copy number becomes high 2. CopB expression increase an Cop represses RepA expression at PrepA 3. CopA now is made-a 90base antisense RNA 4. CopA binds to 5-end of the RepA mRNA, forming dsRNA 5. This is recognized by host RNAaseIII and degraded. Thus concentration of RepA protein is maintained by rate of ...
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