Agilent 380-ELSD Evaporative Light Scattering Detector

1 Agilent 380-ELSD Evaporative Light Scattering Detector IntroductionEvaporative Light Scattering detectors are ideal for detecting analytes with no UVchromophore as they do not rely on the optical properties of a compound. TheAgilent 380-ELSD Evaporative Light Scattering Detector is the latest high perfor-mance ELSD from Agilent , benefitting from almost 20 years experience in the designand manufacture of Evaporative Light Scattering detectors . The Agilent 380-ELSD isthe smallest and most flexible ELSD avaliable. Patented gas flow technology givesthe Agilent 380-ELSD unrivalled detection capability at ambient temperatures anduniform response across a gradient. This precision instrument operates up to 120 C,with a heated nebulizer that makes it a perfect match for a wide range of applica-tions and LC techniques, including GPC/SEC.

2 To detect everything you inject withhigh sensitivity, the Agilent 380-ELSD is the ELSD of choice for all high Benefits High sensitivity provides superb responses for all compounds, down to lownanogram levels. Unrivalled response to semi-volatile compounds at ambient temperature frompatented gas flow technology. Operation up to 120 C gives improved evaporation of difficult solvents and betterresponse to non-volatile compounds. Low dispersion and high-speed data output rates are the perfect match for FastLC applications. Superb reproducibility below 2% gives reliable and accurate results. Full DMSO transparency ensures that responses from early eluting compoundsare not hidden. Data Sheetthere is no need for an analog to digitalconverter.

3 On-the-fly adjustment of lightsource intensity can save time during arun. All this in the smallest footprint avail-able. Being complementary to LC/MS,and offering unrivalled flexibility and sen-sitivity, the Agilent 380-ELSD is the ELSDof choice for demanding, high temperature s Family of EvaporativeLight Scattering DetectorsEvaporative Light Scattering detection isthe powerful alternative to RI and UVfor all compounds; just inject it anddetect it, right down to low nanogramlevels. ELSDs provide a universalresponse independent of the opticalproperties of the analyte so there is noloss of important can use Agilent s ELSDs for a widerange of analytical techniques, includ-ing LC (analytical and preparative),LC/MS, SFC, high throughput screen-ing, GPC/SEC, TREF, and GPEC.

4 Therange of application areas is equallybroad, encompassing pharmaceuticals,nutraceuticals, combinatorial libraries,carbohydrates, lipids, phopspholipids,triglycerides, fatty acids, amino acids,polymers, and non-volatile compounds, wheretemperatures of 100 C or above arerequired, the Agilent 380-ELSD is theinstrument of choice. The Agilent 385-ELSD delivers sub-ambi-ent evaporation down to 10 C, provid-ing maximum sensitivity for compoundswith significant volatility below 30 C. Key Benefits (Continued) Multi-vendor software control anddata acquisition using AgilentChemStation chromatography datasystem, and other vendors inter-faces, eliminates the need for ananalog to digital converter. Fully compatible with all Agilent ana-lytical and preparative LC systemsfor enhanced chromatographic performance.

5 Complementary to LC/MS. The small ELSD footprint frees upbench space for a less cluttered andsafer working environment. Simple set up means that you can be up and running in less than fiveminutes, straight out of the DetailsThe Agilent 380-ELSD is ideal for thedetection of non-volatile compounds,where temperatures of 100 C or aboveare required. The instrument benefitsfrom fast data output rates and extremelylow dispersion for fast LC, and delivers a universal response down to the low-nanogram range for truly representa-tive analysis. Reproducibility is less than 2% for improved consistency of and digital data collection comeas standard for multi-vendor platforms, so2 Choose the Right Agilent ELSD for Your NeedsAgilent 385-ELSDA gilent 380-ELSDHPLC analysis:Non-volatile compounds:yesyesSemi-volatile compounds:yesyesHighly volatile compounds:yesxAmbient GPC analysis:yesyesHigh temperature GPC analysis:xxInstallation Qualification andOperation Qualification (IQOQ) Agilent s ELSDs are rigorously tested toa high specification before they areshipped, and detailed IQOQ documenta-tion is included with every unit.

6 In addi-tion, our detectors can be incorporatedinto any HPLC system, and a completeQualification Workbook is provided tohelp Mass ResponseAgilent ELSDs are not dependent on acompound s optical properties, so theELSD provides a more uniformresponse than UV-VIS, making it theideal Detector for purity analysis orwhere calibration standards are at 280 nmAgilent 380-ELSDC olumn: Polaris C18 150 mm, 5 mEluent: Water/Acetonitrile 1:1 Flow rate: mL/minInjection volume: 10 L Detector : Agilent 380-ELSD (neb = 30 C,evap = 30 C, gas = SLM)Figure 1 The Agilent ELSD delivers a more uniform massresponse than UV from a 1:1 ELSDs - Better byDesignELSD involves a three stage Nebulization Using an inert gasstream to form a plume of uniformlysized droplets.

7 2. Evaporation of the eluent Generatinga plume of non-volatile solute particles. 3. Optical detection Where the intensityof scattered Light is proportional tothe mass of solute passing throughthe optical chamber. NebulizationEfficient nebulization using low gasflow rates is a feature of Agilent nebulizer temperature con-trol and digital gas flow control provideexcellent stability and noise is minimized by theremoval of any poorly nebulized eluentthrough a drain port. EvaporationThe nebulized stream passes through anindependently temperature-controlledevaporator tube where solvent isremoved at high temperature, leavingthe less volatile solute particles Agilent 380-ELSD featurespatented1gas flow control technologywith a short evaporator tube that givesan extremely low swept volume forminimal peak dispersion.

8 This providesmaximum resolution from the separa-tion, especially important for work withsmall columns. Gas inletLiquid wasteEleuentinletLight sourceDetector1UK Patent , US Patent 6/0238744 Optical detectionThe solute particles are detected asthey pass through the optical high power LED and advanceddesign of the electronics delivers maximum components give very poorpeak response due to loss throughevaporation. As the temperatures arereduced to 35 C, and even more so atambient, the Agilent 380-ELSD revealsmuch improved recovery data of thesemi-volatile components, with nodeterioration in baseline : Agilent C18, 150 mm, 5 m Eluent A: TFA in waterEluent B: TFA in AcetonitrileGradient: 60% to 90% in 5 minFlow rate: mL/minInjection volume: 20 LSample concentration: 1 mg/mLDetector: Agilent 380-ELSD (gas= SLM)0min301250 C35 CAmbient34 Figure 4 The ability of the Agilent 380-ELSD to operate atambient improves data :Phenyl hexyl, 50 mm, 5 m Eluent A: g/L Ammonium acetate inwaterEluent B: MethanolGradient: 70% to 100 % B in 4 minTemperature:35 CFlow rate: mL/min Detector .

9 Agilent 380-ELSD (neb = 35 C,evap = 60 C, gas = SLM) Identification1. Erucamide2. Tinuvin 3263. Irgafos 168 4. IrganoxFigure 2 Excellent baseline stability of four polymer : Agilent C18, 4 250 mm, 5 m Eluent A: AcetonitrileEluent B: DichloromethaneGradient: 25% to 45% B in 45 min, 45% to 90% B in 5 min, holdFlow rate: 5 L Detector : Agilent 380-ELSD (neb = 60 C,evap = 50 C, gas = SLM)0min30 Figure 3 Separation of a sample of sesame of Non-volatileCompoundsThe Agilent 380-ELSD is ideal for non-volatile compounds with a weak or nochromophore, such as surfactants, sugars, polymers and lipids, whereevaporation temperatures of > 80 Care of Semi-VolatileCompoundsThe Agilent 380-ELSD is also ideal for separating compounds with differentdegrees of volatility, as shown in Figure 4.

10 With the nebulizer and evapo-rator running at the same temperature,50 C, non-volatile indapamide gives alarge response, but the other more5 DMSO Transparency for HighThroughput ScreeningThe vast majority of combinatoriallibraries screened by high throughputmethods are stored in dimethylsulfox-ide (DMSO). UV and mass spectrome-try will miss potential drug compoundsthat have no UV chromophore there-fore ELSD is commonly used. However,at the low evaporator temperaturesrequired to detect volatile components,the presence of DMSO in the samplecan mask the response of early elutingcompounds. In the Agilent 380-ELSD ,the addition of a carefully controlledstream of gas to the evaporation stepenables complete removal of theDMSO to take place without increasingthe operating : 2 mg/mL in DMSOC olumn: 150 mm, 5 m Eluent A: HFBA in WaterEluent B: HFBA in AcetonitrileGradient: 5% to 30% B in 10 min, 30% to80 % B in 5 minFlow rate: mL/minInjection volume: 20 LDetector: Agilent 380-ELSD (neb = 25 C,evap = 25 C, gas as shown)DMSOGas = SLMGas = SLM0min15 Figure 5 Increasing gas flow on the Agilent 380-ELSD provides DMSO RSDE xcellent reproducibility below 2% givesreliable and accurate results.