Alcohols - SUPELCO

1 318. GC Applications Alcohols 24 5. 1. Methanol 1. 2. Isopropanol 2. 3. 12,13. 3. Ethanol 4. 2-Methyl-2-propanol 3 1. Methanol (Diluent). 6 5. 2-Butanol 2. Oxo-isooctyl Alcohols 9. 8 10 6. 2-Methyl-2-butanol 3. 2-Ethylhexyl Alcohol 7. 1-Propanol 8. 2-Methyl-1-propanol 11 9. 3-Pentanol 7 10. 2-Pentanol 1. 14 11. 1-Butanol 12. 3-Methyl-1-butanol 13. 2-Methyl-1-butanol 14. 1-Pentanol Chromatography 713-0794. 93-0052. 0 4 8 12 16 20 0 4 8 12 16. Min Min Alcohols (GC) Alcohols (GC). Column: PAG, 30m x ID, m film Column: Nukol, 30m x ID, m film Cat. No.: 24223 Cat. No.: 25327. O r d e r : 1 . 8 0 0 . 3 2 5 . 3 0 1 0 Te c h n i c a l S e r v i c e : 1 . 8 0 0 . 3 5 9 . 3 0 4 1 We b : w w w. s i g m a - a l d r i c h . c o m / s u p e l c o Oven: 60 C Oven: 90 C to 160 C at 4 C/min, hold 5 min Carrier: helium, 20cm/sec (set at 60 C), splitter vent flow 65-70cc/min Carrier: helium, 20cm/sec Det.

2 : FID, 260 C Det.: FID, 200 C. Inj.: 1 L Alcohols in water ( each component), Inj.: L methanol, 2% isooctyl Alcohols , 200 C. split 100:1, 250 C. Gas 1. Acetaldehyde 5. n-Propanol 2. 3. 2. Methanol 6. Isobutanol 5 3. Ethanol 7. Active amyl alcohol 1. Acetaldehyde 4. Ethyl acetate 8. Isoamyl alcohol 2. Methanol 6 9. Acetic acid 3 3. Acetone 4 4. Ethanol 1 5. Isopropanol 5. 8 6. n-Propanol 4. 1 6. 7. 9. 2. 796-0380. 713-0622. 0 2 4 6 8 10 12 14 16 18. Min 0 2 4 6. Min Alcohols (GC). Packing: 80/120 Carbopack B AW Carbowax PEG 20M Alcohols , Blood (GC). Cat. No.: 11814 (15g/bottle) Packing: 60/80 Carbopack B/5% Carbowax 20M. Column: 2m x 2mm glass Cat. No.: 11766 (15g/bottle). Oven: 70 C to 150 C at 4 C Column: 6m x 2mm ID glass stock columns available Carrier: nitrogen, 20mL/min Oven: 85 C. Det.: FID Carrier: helium, 20mL/min Inj.: 1 L Scotch whiskey Det.: FID. Inj.: 1 L water, each analyte 5 4.

3 3 1. Acetaldehyde 6 5. 2. Methanol 3. Ethanol 1. 1-Propanol 4. Ethyl acetate 2. 2-Methyl-1-propanol 9 6. 5. n-Propanol 3. 1-Butanol 6. Isobutanol 4. 1-Pentanol 4 7. Acetic acid 3 5. 2-Ethyl-1-butanol 1 8 8. Active amyl alcohol 6. 4-Methyl-2-pentanol 1 2. 9. Isoamyl alcohol 2 7. 796-0381. 0 5 10 15 20. Min 794-0621. Alcohols (GC) Packing: 80/120 Carbopack B / 5% Carbowax 20M Min Cat. No.: 11812-U (15g/bottle). Column: 2m x 2mm ID glass Alcohols (GC). Oven: 70 C to 150 C at 4 C Column: Supel-Q PLOT, 30m x Carrier: nitrogen, 20mL/min Cat. No.: 25462. Det.: FID Oven: 35 C (3 min) to 250 C at 16 C/min Inj.: 1 L Scotch whiskey Carrier: helium, 3mL/min (set at 35 C). Det.: FID, 250 C. Inj.: L. SUPELCO . 319. GC Applications Alcohols 6, 7 1. Acetaldehyde 20. Butanol 2. Methanol 21. Ethyl isobutyrate 3. Propanal 22. 3-Methylbutan-2-ol 4. Acetone 23. 3-Pentanol 12 5. Methyl acetate 24.

4 2-Pentanol 6. Ethanol 25. Isobutyl acetate 7. Isobutanal 26. Ethyl butyrate 8. Butanal 27. Butyl acetate 18. 4 9. Isopropanol 28. 2-Methylpentan-1-ol 24, 25. 9 10. Ethyl acetate 29. 1-Methylpentan-1-ol 11. Diacetal 30. Acetic acid 5 12. n-Propanol 31. Pentanol 1 20 13. Isopentanal 32. Isoamyl acetate 8. 11 15, 16 21 14. sec-Butanol 33. Furfural 2. 33 15. Pentanal 34. Propionic acid Chromatography 22 28 16. Ethyl propionate 35. Hexanol 26 29 31. 17. Propyl acetate 36. Isobutyric acid 3 13 30 35 36 37. 17 23 18. Isobutanol 37. Butyric acid 10 14 34. 27 32 19. Acetal 19. O r d e r : 1 . 8 0 0 . 3 2 5 . 3 0 1 0 Te c h n i c a l S e r v i c e : 1 . 8 0 0 . 3 5 9 . 3 0 4 1 We b : w w w. s i g m a - a l d r i c h . c o m / s u p e l c o 0 2 4 6 8 10 12 14 16 18 20 22 24 26. 713-0711. Min Use this packing in glass columns only. For more information, request Bulletin 790. Figure provided by Dr.

5 Antonio DiCorcia and colleagues, University of Rome (used with permission of Elsevier Scientific Publishing Co., Amsterdam). Gas Alcoholic Beverage/Fermentation Components (GC). Packing: 80/120 Carbopack B PEG 20M. Cat. No.: 11814 (15g/bottle). Column: 2m x 2mm ID glass Oven: 80 C to 200 C at 4 C/min Carrier: nitrogen Det.: FID. Inj.: 1 L water:ethanol (50:50), 40-60ppm each analyte 21 23 25 28 1. Methanol Excellent Peak Shapes and Resolutions 2. Ethanol 18 3. Isopropanol 22. 1 24 4. t-Butanol 26 27. 8 29 5. Propanol 9 19 6. 2-Butanol 2. 7. Hexane 13 30 8. Isobutanol U. Unkonwn 5 9. Butanol 11,12 16. 10. 3-Methyl-2-butanol 20. 11. 2-Pentanol 17 31. 14. 15. 12. 3-Pentanol 14. 13 13. Heptane 7 14. 3-Methyl-1-butanol 4. 10 15. 2-Methyl-1-butanol 32. 16. 3-Methyl-3-pentanol 3. 6 17. 4-Methyl-2-pentanol 18. Pentanol 19. Octane 20. 4-Methyl-1-pentanol 21. Hexanol 22. Nonane 23.

6 Heptanol 24. Decane 25. Octanol 26. Undecane U. 27. Dodecane 28. Decanol 29. Tridecane G001709. 30. Tetradecane 0 10 Min 20 Min 30 31. Pentadecane 32. Hexadecane Hydrocarbons and Alcohols (GC). Column: Equity-1, 30m x ID, m Cat. No.: 28076-U. Oven: 40 C (5 min) to 225 C @ 8 C /min Inj.: 250 C. Det.: FID, 275 C. Flow: Helium, 30 cm/sec @ 40 C. Injection: L, split 100:1. Liner: Split, cup design Sample: 32 component mixed solvent sample, equal by weight SUPELCO . 320. GC Applications Alcohols , Amines 1-Phenyl-2-propanol Isopinocampheol 4 5 6 7. 8. 3 1. Propylamine 2. Butylamine + 2. 3. Pentylamine 4. Hexylamine 5. Heptylamine - 6. Octylamine 1 7. Nonylamine 8. Decylamine Chromatography 18 20 30 32. Min 6-Methyl-5-hepten-2-ol Min 797-0021, 0022, 0023, 0020, 0025, 0024. 711-0127. 0 2 4 6 8 10 12 14. Min O r d e r : 1 . 8 0 0 . 3 2 5 . 3 0 1 0 Te c h n i c a l S e r v i c e : 1.

7 8 0 0 . 3 5 9 . 3 0 4 1 We b : w w w. s i g m a - a l d r i c h . c o m / s u p e l c o Amines, Primary (GC). Column: Carbowax Amine, 30m x ID, m film Cat. No.: 25353. Gas Oven: 50 C to 200 C at 8 C/min, hold 10 min Carrier: helium, 20cm/sec (set at 170 C). 13 15 Det.: FID, 220 C. Min Inj.: L neat amines mix, split (100:1), 220 C. Alcohol Enantiomers (GC). Column: b -DEX 325, 30m x ID, m film Cat. No.: 24308. Oven: 110 C, 1-phenyl-2-propanol; 100 C, isopinocampheol; 1. Nitrosodimethylamine 90 C, 6-methyl-5-hepten-2-ol 2. Nitrosodiethylamine Carrier: helium, 20cm/sec set at the analysis temperature 3. Nitrosomethylethylamine Det.: FID, 300 C 4. Nitrosodipropylamine Inj.: 1 L methylene chloride (1mg/mL each analyte), split (100:1), 220 C 5. Nitrosopiperidine 6. Nitrosodibutylamine 7. Nitrosodiphenylamine 4 5 6 7. 3 4. 1. Methylamine 2. Dimethylamine IS Isopropanol (Internal Standard) 3.

8 3. Diethylamine 2. 4. Triethylamine 1. 1 2. 4 6 8 10 12 14 16 18 20. 96-0142. Min Chromatogram courtesy of J. Clark, Liggett Group, Inc. IS. Nitrosamines (SPME/GC). 95-0065. 1 2 3 4 5 6 7 8 Sample: analytes in water +25% KCl, pH Min SPME Fiber: polydimethylsiloxane/divinylbenzene, 65 m film Cat. No.: 57310-U. Amines (SPME/GC) Extraction: immersion, 15min (rapid stirring). Desorption: 270 C, 1min Sample: amines at 5ppm in water, 27% NaCl, pH Column: PTA-5, 30m x ID, m film SPME Fiber: polydimethylsiloxane/divinylbenzene, 65 m film Cat. No.: 24331. Cat. No.: 57310. Oven: 50 C (1min) to 250 C at 10 C/min, hold 2 min Extraction: 20 min, fiber immersed in water, rapid stirring Carrier: helium, 30cm/sec Desorption: 5 min, 270 C. Column: SPB-1 SULFUR, 30m x ID, m film Det.: GC/MS (quadrupole, selected ion mode). Cat. No.: 24158 Inj.: splitless, 250 C ( ID liner). Oven: 50C (2min) to 150 C at 10 C/min Carrier: helium, 25cm/sec (set at 50 C).

9 Det.: GC/MS, selected ions at sec/scan Inj.: splitless/split, closed initial 5 min, 270 C, ID splitless liner SUPELCO . 321. GC Applications Anilines, Aromatics, Alditol Acetates 1. Aniline Excellent Peak Shapes 2. 3-Aminobenzotrifluoride and Resolutions 3. o-Toluidine 4. N,N-Dimethylaniline 11. 8 9 5. 2-Chloroaniline 6 6. 2,6-Dimethylaniline 7. 3-Chloro-4-Fluoroaniline 3 4 8. 4-Isopropylaniline 9. 2-Methyl-6-Ethylaniline 10 10. 4-Chloro-4-Methylaniline 5 11. 2,6-Diethylaniline 12. 2,4-Diaminotoluene 1 7 13. 3,4-Dichloroaniline 16. 2 13. 12 14 14. 2,4,5-Trichloroaniline 15. 4-Chloro-2-nitroaniline 15. Chromatography 16. 3,3'-Dichlorobenzidine pA. Bleed @ 325 C. O r d e r : 1 . 8 0 0 . 3 2 5 . 3 0 1 0 Te c h n i c a l S e r v i c e : 1 . 8 0 0 . 3 5 9 . 3 0 4 1 We b : w w w. s i g m a - a l d r i c h . c o m / s u p e l c o 8 10 12 14 16 18 20 22 24. G001706 Min Anilines (GC).

10 Column: Equity-5, 30m x ID, m Gas Cat. No.: 28089-U. Oven: 50 C (2 min) to 200 C @ 10 C/min to 325 C @ 15 C/min 1 2. 3. Inj.: 250 C 4. 7,8 1. Rhamnitol Det.: FID, 325 C 1 2. Fucitol Flow: Helium, constant flow, mL/sec @ 50 C 3. Ribitol Injection: L, splitless ( min) 4. Arabinitol Liner: Splitless, 4mm ID single taper 5. Inositol Sample: 50ng on-column of a custom anilines mix 5 6. Mannitol 6 7. Glucitol 8. Galactitol Healthy Plant 1000 2000 3000 4000 5000 6000 713-0912. 0 4 8 12 16 20 24. Wounded Plant Min Alditol Acetates (GC). Column: SPB-1701, 30m x ID, m film 796-0153,0154. Cat. No.: 24113. 1000 2000 3000 4000 5000 6000 Oven: 220 C. Carrier: helium, 20cm/sec (set at 220 C). Figure provided by Borg-Karlson, The Royal Institute of Technology, Department of Chemistry, Stockholm, Sweden. Det.: FID, 260 C. Inj.: 1 L, split 100:1 (5ng each compound on column). Mono- and Sesquiterpenes in Spruce Seedlings (SPME/GC).