Alcohols - SUPELCO

1 318. GC Applications Alcohols 24 5. 1. Methanol 1. 2. Isopropanol 2. 3. 12,13. 3. Ethanol 4. 2-Methyl-2-propanol 3 1. Methanol (Diluent). 6 5. 2-Butanol 2. Oxo-isooctyl Alcohols 9. 8 10 6. 2-Methyl-2-butanol 3. 2-Ethylhexyl Alcohol 7. 1-Propanol 8. 2-Methyl-1-propanol 11 9. 3-Pentanol 7 10. 2-Pentanol 1. 14 11. 1-Butanol 12. 3-Methyl-1-butanol 13. 2-Methyl-1-butanol 14. 1-Pentanol Chromatography 713-0794. 93-0052. 0 4 8 12 16 20 0 4 8 12 16. Min Min Alcohols (GC) Alcohols (GC). Column: PAG, 30m x ID, m film Column: Nukol, 30m x ID, m film Cat. No.: 24223 Cat. No.: 25327. O r d e r : 1 . 8 0 0 . 3 2 5 . 3 0 1 0 Te c h n i c a l S e r v i c e : 1 . 8 0 0 . 3 5 9 . 3 0 4 1 We b : w w w. s i g m a - a l d r i c h . c o m / s u p e l c o Oven: 60 C Oven: 90 C to 160 C at 4 C/min, hold 5 min Carrier: helium, 20cm/sec (set at 60 C), splitter vent flow 65-70cc/min Carrier: helium, 20cm/sec Det.: FID, 260 C Det.: FID, 200 C. Inj.: 1 L Alcohols in water ( each component), Inj.

2 : L methanol, 2% isooctyl Alcohols , 200 C. split 100:1, 250 C. Gas 1. Acetaldehyde 5. n-Propanol 2. 3. 2. Methanol 6. Isobutanol 5 3. Ethanol 7. Active amyl alcohol 1. Acetaldehyde 4. Ethyl acetate 8. Isoamyl alcohol 2. Methanol 6 9. Acetic acid 3 3. Acetone 4 4. Ethanol 1 5. Isopropanol 5. 8 6. n-Propanol 4. 1 6. 7. 9. 2. 796-0380. 713-0622. 0 2 4 6 8 10 12 14 16 18. Min 0 2 4 6. Min Alcohols (GC). Packing: 80/120 Carbopack B AW Carbowax PEG 20M Alcohols , Blood (GC). Cat. No.: 11814 (15g/bottle) Packing: 60/80 Carbopack B/5% Carbowax 20M. Column: 2m x 2mm glass Cat. No.: 11766 (15g/bottle). Oven: 70 C to 150 C at 4 C Column: 6m x 2mm ID glass stock columns available Carrier: nitrogen, 20mL/min Oven: 85 C. Det.: FID Carrier: helium, 20mL/min Inj.: 1 L Scotch whiskey Det.: FID. Inj.: 1 L water, each analyte 5 4. 3 1. Acetaldehyde 6 5. 2. Methanol 3. Ethanol 1. 1-Propanol 4. Ethyl acetate 2. 2-Methyl-1-propanol 9 6. 5. n-Propanol 3. 1-Butanol 6.

3 Isobutanol 4. 1-Pentanol 4 7. Acetic acid 3 5. 2-Ethyl-1-butanol 1 8 8. Active amyl alcohol 6. 4-Methyl-2-pentanol 1 2. 9. Isoamyl alcohol 2 7. 796-0381. 0 5 10 15 20. Min 794-0621. Alcohols (GC) Packing: 80/120 Carbopack B / 5% Carbowax 20M Min Cat. No.: 11812-U (15g/bottle). Column: 2m x 2mm ID glass Alcohols (GC). Oven: 70 C to 150 C at 4 C Column: Supel-Q PLOT, 30m x Carrier: nitrogen, 20mL/min Cat. No.: 25462. Det.: FID Oven: 35 C (3 min) to 250 C at 16 C/min Inj.: 1 L Scotch whiskey Carrier: helium, 3mL/min (set at 35 C). Det.: FID, 250 C. Inj.: L. SUPELCO . 319. GC Applications Alcohols 6, 7 1. Acetaldehyde 20. Butanol 2. Methanol 21. Ethyl isobutyrate 3. Propanal 22. 3-Methylbutan-2-ol 4. Acetone 23. 3-Pentanol 12 5. Methyl acetate 24. 2-Pentanol 6. Ethanol 25. Isobutyl acetate 7. Isobutanal 26. Ethyl butyrate 8. Butanal 27. Butyl acetate 18. 4 9. Isopropanol 28. 2-Methylpentan-1-ol 24, 25. 9 10. Ethyl acetate 29. 1-Methylpentan-1-ol 11.

4 Diacetal 30. Acetic acid 5 12. n-Propanol 31. Pentanol 1 20 13. Isopentanal 32. Isoamyl acetate 8. 11 15, 16 21 14. sec-Butanol 33. Furfural 2. 33 15. Pentanal 34. Propionic acid Chromatography 22 28 16. Ethyl propionate 35. Hexanol 26 29 31. 17. Propyl acetate 36. Isobutyric acid 3 13 30 35 36 37. 17 23 18. Isobutanol 37. Butyric acid 10 14 34. 27 32 19. Acetal 19. O r d e r : 1 . 8 0 0 . 3 2 5 . 3 0 1 0 Te c h n i c a l S e r v i c e : 1 . 8 0 0 . 3 5 9 . 3 0 4 1 We b : w w w. s i g m a - a l d r i c h . c o m / s u p e l c o 0 2 4 6 8 10 12 14 16 18 20 22 24 26. 713-0711. Min Use this packing in glass columns only. For more information, request Bulletin 790. Figure provided by Dr. Antonio DiCorcia and colleagues, University of Rome (used with permission of Elsevier Scientific Publishing Co., Amsterdam). Gas Alcoholic Beverage/Fermentation Components (GC). Packing: 80/120 Carbopack B PEG 20M. Cat. No.: 11814 (15g/bottle). Column: 2m x 2mm ID glass Oven: 80 C to 200 C at 4 C/min Carrier: nitrogen Det.

5 : FID. Inj.: 1 L water:ethanol (50:50), 40-60ppm each analyte 21 23 25 28 1. Methanol Excellent Peak Shapes and Resolutions 2. Ethanol 18 3. Isopropanol 22. 1 24 4. t-Butanol 26 27. 8 29 5. Propanol 9 19 6. 2-Butanol 2. 7. Hexane 13 30 8. Isobutanol U. Unkonwn 5 9. Butanol 11,12 16. 10. 3-Methyl-2-butanol 20. 11. 2-Pentanol 17 31. 14. 15. 12. 3-Pentanol 14. 13 13. Heptane 7 14. 3-Methyl-1-butanol 4. 10 15. 2-Methyl-1-butanol 32. 16. 3-Methyl-3-pentanol 3. 6 17. 4-Methyl-2-pentanol 18. Pentanol 19. Octane 20. 4-Methyl-1-pentanol 21. Hexanol 22. Nonane 23. Heptanol 24. Decane 25. Octanol 26. Undecane U. 27. Dodecane 28. Decanol 29. Tridecane G001709. 30. Tetradecane 0 10 Min 20 Min 30 31. Pentadecane 32. Hexadecane Hydrocarbons and Alcohols (GC). Column: Equity-1, 30m x ID, m Cat. No.: 28076-U. Oven: 40 C (5 min) to 225 C @ 8 C /min Inj.: 250 C. Det.: FID, 275 C. Flow: Helium, 30 cm/sec @ 40 C. Injection: L, split 100:1. Liner: Split, cup design Sample: 32 component mixed solvent sample, equal by weight SUPELCO .

6 320. GC Applications Alcohols , Amines 1-Phenyl-2-propanol Isopinocampheol 4 5 6 7. 8. 3 1. Propylamine 2. Butylamine + 2. 3. Pentylamine 4. Hexylamine 5. Heptylamine - 6. Octylamine 1 7. Nonylamine 8. Decylamine Chromatography 18 20 30 32. Min 6-Methyl-5-hepten-2-ol Min 797-0021, 0022, 0023, 0020, 0025, 0024. 711-0127. 0 2 4 6 8 10 12 14. Min O r d e r : 1 . 8 0 0 . 3 2 5 . 3 0 1 0 Te c h n i c a l S e r v i c e : 1 . 8 0 0 . 3 5 9 . 3 0 4 1 We b : w w w. s i g m a - a l d r i c h . c o m / s u p e l c o Amines, Primary (GC). Column: Carbowax Amine, 30m x ID, m film Cat. No.: 25353. Gas Oven: 50 C to 200 C at 8 C/min, hold 10 min Carrier: helium, 20cm/sec (set at 170 C). 13 15 Det.: FID, 220 C. Min Inj.: L neat amines mix, split (100:1), 220 C. Alcohol Enantiomers (GC). Column: b -DEX 325, 30m x ID, m film Cat. No.: 24308. Oven: 110 C, 1-phenyl-2-propanol; 100 C, isopinocampheol; 1. Nitrosodimethylamine 90 C, 6-methyl-5-hepten-2-ol 2.

7 Nitrosodiethylamine Carrier: helium, 20cm/sec set at the analysis temperature 3. Nitrosomethylethylamine Det.: FID, 300 C 4. Nitrosodipropylamine Inj.: 1 L methylene chloride (1mg/mL each analyte), split (100:1), 220 C 5. Nitrosopiperidine 6. Nitrosodibutylamine 7. Nitrosodiphenylamine 4 5 6 7. 3 4. 1. Methylamine 2. Dimethylamine IS Isopropanol (Internal Standard) 3. 3. Diethylamine 2. 4. Triethylamine 1. 1 2. 4 6 8 10 12 14 16 18 20. 96-0142. Min Chromatogram courtesy of J. Clark, Liggett Group, Inc. IS. Nitrosamines (SPME/GC). 95-0065. 1 2 3 4 5 6 7 8 Sample: analytes in water +25% KCl, pH Min SPME Fiber: polydimethylsiloxane/divinylbenzene, 65 m film Cat. No.: 57310-U. Amines (SPME/GC) Extraction: immersion, 15min (rapid stirring). Desorption: 270 C, 1min Sample: amines at 5ppm in water, 27% NaCl, pH Column: PTA-5, 30m x ID, m film SPME Fiber: polydimethylsiloxane/divinylbenzene, 65 m film Cat. No.: 24331. Cat. No.: 57310. Oven: 50 C (1min) to 250 C at 10 C/min, hold 2 min Extraction: 20 min, fiber immersed in water, rapid stirring Carrier: helium, 30cm/sec Desorption: 5 min, 270 C.

8 Column: SPB-1 SULFUR, 30m x ID, m film Det.: GC/MS (quadrupole, selected ion mode). Cat. No.: 24158 Inj.: splitless, 250 C ( ID liner). Oven: 50C (2min) to 150 C at 10 C/min Carrier: helium, 25cm/sec (set at 50 C). Det.: GC/MS, selected ions at sec/scan Inj.: splitless/split, closed initial 5 min, 270 C, ID splitless liner SUPELCO . 321. GC Applications Anilines, Aromatics, Alditol Acetates 1. Aniline Excellent Peak Shapes 2. 3-Aminobenzotrifluoride and Resolutions 3. o-Toluidine 4. N,N-Dimethylaniline 11. 8 9 5. 2-Chloroaniline 6 6. 2,6-Dimethylaniline 7. 3-Chloro-4-Fluoroaniline 3 4 8. 4-Isopropylaniline 9. 2-Methyl-6-Ethylaniline 10 10. 4-Chloro-4-Methylaniline 5 11. 2,6-Diethylaniline 12. 2,4-Diaminotoluene 1 7 13. 3,4-Dichloroaniline 16. 2 13. 12 14 14. 2,4,5-Trichloroaniline 15. 4-Chloro-2-nitroaniline 15. Chromatography 16. 3,3'-Dichlorobenzidine pA. Bleed @ 325 C. O r d e r : 1 . 8 0 0 . 3 2 5 . 3 0 1 0 Te c h n i c a l S e r v i c e : 1.

9 8 0 0 . 3 5 9 . 3 0 4 1 We b : w w w. s i g m a - a l d r i c h . c o m / s u p e l c o 8 10 12 14 16 18 20 22 24. G001706 Min Anilines (GC). Column: Equity-5, 30m x ID, m Gas Cat. No.: 28089-U. Oven: 50 C (2 min) to 200 C @ 10 C/min to 325 C @ 15 C/min 1 2. 3. Inj.: 250 C 4. 7,8 1. Rhamnitol Det.: FID, 325 C 1 2. Fucitol Flow: Helium, constant flow, mL/sec @ 50 C 3. Ribitol Injection: L, splitless ( min) 4. Arabinitol Liner: Splitless, 4mm ID single taper 5. Inositol Sample: 50ng on-column of a custom anilines mix 5 6. Mannitol 6 7. Glucitol 8. Galactitol Healthy Plant 1000 2000 3000 4000 5000 6000 713-0912. 0 4 8 12 16 20 24. Wounded Plant Min Alditol Acetates (GC). Column: SPB-1701, 30m x ID, m film 796-0153,0154. Cat. No.: 24113. 1000 2000 3000 4000 5000 6000 Oven: 220 C. Carrier: helium, 20cm/sec (set at 220 C). Figure provided by Borg-Karlson, The Royal Institute of Technology, Department of Chemistry, Stockholm, Sweden. Det.: FID, 260 C.

10 Inj.: 1 L, split 100:1 (5ng each compound on column). Mono- and Sesquiterpenes in Spruce Seedlings (SPME/GC). Sample: headspace surrounding Picea abies seedlings SPME Fiber: polydimethylsiloxane, 100 m film Cat. No.: 57300-U. Extraction: headspace, 60 min Desorption: 30 sec, 225 C. Column: poly(5% diphenyl/95% dimethylsiloxane), 30m x ID, m film SUPELCO Equivalent: Equity-5 (Cat. No. 28089-U). Oven: 40 C (4 min) to 200 C at 4 C/min, hold 16 min Carrier: helium, 10psi Det.: EID, 70ev Inj.: splitless (splitter opened after 30 sec), 225 C. SUPELCO . 322. GC Applications Alditol Acetates, Drugs 1. Amphetamine 9. Amitripyline Amphetamine: 2. Methamphetamine 10. Cocaine Excellent Response 3. Nicotine 11. Desipramine 5 6. and Peak Symmetry 4. Caffeine 12. Codeine 5. Diphenhydramine 13. Morphine 6. Lidocaine 14. Diazepam 3 9. 7. Phenobarbital 15. Heroin 8 8. Methadone 16. Fentenyl 10. Chromatography 4 14. 1. 12 Key Resolutions 7 Achieved 2. O r d e r : 1.