BD Cytometric Bead Array (CBA) Product Catalog

1 For Research Use Only. Not for use in diagnostic or therapeutic BiosciencesBD Cytometric Bead Array (CBA) Product ListFor Research Use Only. Not for use in diagnostic or therapeutic procedures. Not for resale. All applications are either tested in-house or reported in the literature. See Technical Data Sheets for of BD CBA ProductsReagents with Superior Quality and ReproducibilityAll BD CBA products are evaluated for performance characteristics including reproducibility, linearity, spike recovery, and theoretical limit of detection. These values are reported in the Product documentation that is included with each BD CBA NIBSC protein standards are recognized by the World Health Organization (WHO) as international biological standards.

2 They meet established requirements for accuracy, consistency, and stability. The NIBSC/WHO standards are assigned potency values in International Units (IU) of biological activity and nominal mass (ie, not absolute mass values). Therefore they cannot be used to establish absolute concentrations for a cytokine preparation. However, these standards do provide a means to facilitate comparisons of cytokine concentration values determined by experiments conducted within different laboratories or source of a recombinant protein (ie, insect cell, E. coli, etc) and the affinity of antibodies used can affect the measurement and performance of a protein in an immunoassay.

3 The conversion factors provided in the subsequent tables make it possible to compare protein concentrations in samples measured by different immunoassays that have been standardized to the same NIBSC/WHO conversion factor may change based on the batch of either standard. Therefore, the conversion factor is intended to be a guideline indicating whether a BD CBA assay over- or underestimates analyte concentrations relative to the NIBSC/WHO standards. Researchers are advised to incorporate both sets of standards in their assays if they wish to derive data from the NIBSC/WHO of BD CBA Standards to the NIBSC/WHO International StandardsTitration curve comparing the BD CBA Human IFN-g recombinant standard to the NIBSC/WHO International CBAS tandardNIBSC/WHOI nternational StandardConcentration(pg/mL)Median Fluorescence Intensity(MFI)Reproducibility The intra-assay and inter-assay reproducibility are determined by evaluating ten replicates of three different sample levels (intra-assay) and three replicates of three different sample levels from four different experiments (inter-assay).

4 Linearity Cell culture supernatant, serum, or EDTA-plasma is spiked with protein and serially diluted. The diluted samples are assayed and the results are compared with the values expected from each recovery Cell culture supernatant, serum, or EDTA-plasma is spiked with three different levels of protein. The samples are assayed and the results are compared to the expected values to determine limit of detection The individual standard curve range for a given cytokine defines the minimum and maximum quantifiable levels using a BD CBA assay. The theoretical limit of detection is defined as the corresponding concentration at two standard deviations above the median fluorescence of 30 replicates of the negative control (0 pg/mL) using a 4- or 5-parameter Research Use Only.

5 Not for use in diagnostic or therapeutic procedures. Not for resale. All applications are either tested in-house or reported in the literature. See Technical Data Sheets for of BD CBA Products (continued)Table 1. NIBSC conversion factor summary for BD CBA Flex Set StandardsSpecificitynibSc Standardcalculated concentration uSing bd cba flex Set (pg/ml)nominal nibSc concentration (pg/ml)bd cba flex Set: nibSc/who maSS converSion factorcode unitS / vialiu valueHuman b-FGF90-7124 g1,6001, , G-CSF88-502100 ng10,0001, , GM-CSF88-6461 g10,0001, , ng2501, , IL-1b86-6801 g100,0002, , ng1002, , IL-391-5101 g1,7001, , IL-488-656100 ng1,0001, , IL-590-586500 ng5,0001, , IL-689-5481 g100,0002, , IL-790-5301 g100, , IL-889-5201 g1,0002, , IL-991-678100 ng1, , IL-1093-7221 g5,0002, , IL-1192-788500 ng5,00013, 1, , IL-12p7095-5441 g10,0001, , IL-1395-6221 g1,0002, , MCP-192-7945 g5,000 arbitrary units2, , MIP-1a92-5182 g200 arbitrary units1, , OSM93-5641 g25.

6 RANTES92-52010 g100,000 arbitrary units1, , TNF88-7861 g46,5001, , TNFRI96-52810 gnot provided12, 1, , TNFRII93-52410 gnot , VEGF02-28613 g13,0001, , GM-CSF91-6581 g100,000 arbitrary units1, , IL-1b93-668100 ng100,000 arbitrary units4, , IL-293-566100 ng10,000 arbitrary , IL-391-6621 g100,000 arbitrary units1, , IL-491-6561 g10,000 arbitrary , IL-693-730100 ng10,000 arbitrary , TNF88-5321 g200,000 arbitrary units 1, , Research Use Only. Not for use in diagnostic or therapeutic procedures. Not for resale. All applications are either tested in-house or reported in the literature. See Technical Data Sheets for 2.

7 NIBSC conversion factor summary for BD CBA Kit StandardsSpecificitynibSc Standardcalculated concentration uSing bd cba kit (pg/ml)nominal nibSc concentration (pg/ml)bd cba kit: nibSc/who maSS converSion factorcode unitS / vialiu valueHuman ng2504, , IL-1b86-6801 g100,0003, , ng1003, , IL-488-656100 ng1,0005, , IL-590-586500 ng5,0003, , IL-689-5481 g100,0004, , IL-889-5201 g1,0003, , IL-1093-7221 g5,0004, , IL-12p7095-5441 g10,0003, , MCP-192-7945 g5,000 arbitrary units2, , RANTES92-52010 g100,000 arbitrary units 1, , TNF88-7861 g46,5003, , IL-293-566100 ng10,000 arbitrary , IL-491-6561 g10,000 arbitrary , IL-693-730100 ng10,000 arbitrary , TNF88-5321 g200,000 arbitrary units2, , of BD CBA Products (continued)For Research Use Only.

8 Not for use in diagnostic or therapeutic procedures. Not for resale. All applications are either tested in-house or reported in the literature. See Technical Data Sheets for CBA Product ListBD CBA Flex SetsCell Signaling Human, Mouse, RatHuman GAPDH Flex Set (Bead A9) Hu FCM RUO 100 tests BDB560792 Phospho BLNK (Y84) Flex Set (Bead C9) Hu FCM RUO 100 tests BDB560063 Phospho Btk (Y551) Flex Set (Bead D5) Hu FCM RUO 100 tests BDB560004 Phospho c-Jun (S63)

9 Flex Set (Bead D8) Hu FCM RUO 100 tests BDB560059 Phospho eNos (S1177) Flex Set (Bead C7) Hu, Ms FCM RUO 100 tests BDB560021 Phospho eNos (T495) Flex Set (Bead C7) Hu FCM RUO 100 tests BDB560065 Phospho ERK1/2 (T202/Y204) Flex Set (Bead C4) Hu, Ms, Rat FCM RUO 100 tests BDB560012 Phospho Itk (Y511) Flex Set (Bead C6) Hu, Ms, Rat FCM RUO 100 tests BDB560008 Phospho JNK1/2 (T183/Y185) Flex Set (Bead B5) Hu, Ms, Rat FCM RUO 100 tests BDB560013 Phospho MEK1/2 (S222) Flex Set (Bead A6) Hu FCM RUO 100 tests BDB560150 Phospho p38 (T180/Y182) Flex Set (Bead B6) Hu, Ms, Rat FCM RUO 100 tests BDB560010 Phospho PLC-g (Y783) Flex Set (Bead B7)

10 Hu, Ms, Rat FCM RUO 100 tests BDB560009 Phospho Rsk (T573) Flex Set (Bead D7) Hu, Ms, Rat FCM RUO 100 tests BDB560024 Phospho SLP-76 (Y145) Flex Set (Bead D6) Hu FCM RUO 100 tests BDB560067 Phospho Stat1 (Y701) Flex Set (Bead C5) Hu FCM RUO 100 tests BDB560015 Phospho Stat3 (Y705) Flex Set (Bead C8) Hu FCM RUO 100 tests BDB560093 Phospho Syk (Y352) Flex Set (Bead B9) Hu, Ms, Rat FCM RUO 100 tests BDB560014 Phospho ZAP-70 (Y319) Flex Set (Bead B8) Hu, Ms FCM RUO 100 tests BDB560011 Total Jnk Flex Set (Bead B5) Hu FCM RUO 100 tests BDB560214 Total p38-a Flex Set (Bead B6)