Human Inflammatory Cytokine Standards - pufei.com

1 BD cytometric Bead array (CBA)Technical Data SheetHuman Inflammatory Cytokine StandardsProduct InformationMaterial Number:552932 Size: ml lyophilizedLyophilized in an aqueous buffered solution containing BSA and ProClin Buffer:DescriptionThe Human Inflammatory Cytokine Standards contain a lyophilized mixture of recombinant Human Interleukin (IL)-8, IL-1 , IL-6, IL-10, Tumor Necrosis Factor (TNF), and IL-12p70. The reconstituted Human Inflammatory Cytokine Standards are useful as a standard control with the BD cytometric Bead array (CBA) Human Inflammatory Cytokine Kit (Cat.)

2 No. 551811). Please refer to the manual included with the BD CBA Human Inflammatory Cytokine Kit for further information. Preparation and StorageStore undiluted at 4 Human Inflammatory Cytokine Standards were mixed at a concentration of 50 ng of each protein/ml and lyophilized under optimal conditions. Following reconstitution, store the freshly reconstituted standard at 4 C and use within 12 of Human Inflammatory Cytokine Standard DilutionsApplication NotesRecommended Assay Procedure: The Human Inflammatory Cytokine Standards have been tested with the BD CBA Human Inflammatory Cytokine Kit to assure protein amounts and function in the BD CBA assay.

3 The lyophilized Standards should be reconstituted using the Assay Diluent (Cat. No. 51-2432KC) supplied in the BD CBA Human Inflammatory Cytokine Kit. Refer to the Preparation of Human Inflammatory Cytokine Standards on this sheet or in Section 6 of the BD CBA Human Inflammatory Cytokine Kit (Cat. No. 551811) : Preparation of Human Inflammatory Cytokine Standards : The Human Inflammatory Cytokine Standards are lyophilized and should be reconstituted and serially diluted before mixing with the Capture beads and the PE Detection Reagent.

4 1. Reconstitute 1 vial of lypholized Human Inflammatory Cytokine Standards with ml of Assay Diluent to prepare a 10x bulk standard. Allow the reconstituted standard to equilibrate for at least 15 minutes before making dilutions. Agitate vial to mix thoroughly. Do not vortex, mix by pipet Label 12 x 75 mm tubes (BD Falcon, Cat. No. 352008) and arrange them in the following order: Top Standard, 1:2, 1:4, 1:8, 1:16, 1:32, 1:64, 1:128, and 1:256. 3. Add 900 l of Assay Diluent to the Top Standard tube.

5 4. Add 300 l of Assay Diluent to each of the remaining tubes. 5. Transfer 100 l of 10x bulk standard to the Top Standard tube and mix Rev. 1 Page 1 of 26. Perform a serial dilution by transferring 300 l from the Top Standard to the 1:2 dilution tube and mix thoroughly. Do not vortex, mix by pipet only. Continue making serial dilutions by transferring 300 l from the 1:2 tube to the 1:4 tube and so on to the 1:256 tube and mix thoroughly. The Assay Diluent serves as the negative approximate concentration (pg/ml) of recombinant protein in each dilution tube is shown in Figure Inflammatory Cytokine Standard Concentrations after DilutionProduct NoticesSince applications vary, each investigator should titrate the reagent to obtain optimal results.

6 1. Please refer to for technical protocols. 2. Source of all serum proteins is from USDA inspected abattoirs located in the United States. 3. ProClin is a trademark of Rohm and Haas Company. 4. Hazardous Ingredient: ProClin 150. Avoid exposure to skin and eyes and ingestion. Wash exposed skin with soap and water. Flush eyes with water. 5. 552932 Rev. 1 Page 2 of 2