Biotechnology Explorer™ Forensic DNA ... - Bio-Rad

1 Biotechnology explorer Forensic DNA Fingerprinting KitInstruction ManualCatalog #166-0077 EDUThe kit is shipped at room temperature. Open immediately upon arrival and store reagent bag at 20 C within 3 weeks of of any part of this document is permitted for classroom use visit to access our selection of language translations for Biotechnology explorer kit DNA evidence solve human problems?DNA fingerprinting is now used routinely to solve crimes. In recent years, news stories havereported how miniscule amounts of DNA have been used to identify individuals involved inincidents even many years in the past, as well as exonerate innocent people from power of DNA as a tool for individual identification captures students activity provides in-depth instruction about how restriction enzymes cleave DNA, howelectrophoresis is used to separate and visualize DNA fragments, and how these techniquescan be combined to obtain a DNA fingerprint.

2 Principles of restriction analysis, plasmidmapping and DNA fragment size determination can also be documented with this the door to rich discussions about scientific, ethical, and legal implications of DNAprofiling. DNA fingerprinting is used in medical and Forensic procedures, as well as inpaternity determinations to discern genetic relationships between individuals at the molecularlevel. This kit allows students to play the role of a Forensic scientist and make a positiveID that is, to simulate using real DNA as evidence and figure out for themselves: Whodone it?

3 In this activity, students analyze six different samples of plasmid DNA. One sample collectedfrom a hypothetical crime scene and five samples obtained from suspects are digestedwith two restriction enzymes. The resulting DNA fragments are separated and visualized inagarose gels using Bio-Rad s Fast Blast DNA stain. Based on the restriction fragment patterns, students compare the evidence and match one of the suspects DNA tothe sample collected at the crime an alternative to the classical human Forensic applications for this kit, have your studentsimagine they are high tech pathologists investigating an outbreak of an aggressive infectiousdisease that has never been seen before.

4 The Centers for Disease Control and Preventionsuspects that a new strain of bacteria has arisen that not only is the cause of the new disease,but also has acquired multiple resistance plasmids from some other bacterial strains. Theirjob is to develop a DNA diagnostic tool for identifying the culprit plasmids. They decide touse restriction enzyme analysis and DNA electrophoresis fingerprinting to identify and distinguish different suspect plasmids and track their spread through the environment. DNAfrom the cultures of a number of stricken patients has been isolated.

5 Have your studentsidentify the new killer bug before the pathogen gets out into the general population andstarts a true epidemic!We strive to continually improve our Biotechnology explorer kits and curricula. Pleaseshare your stories, comments and suggestions!You can download this complete instruction manual on the Internet. Visit us on the Web or call us in the US at curriculum was developed in collaboration with Len Poli and Russ Janigian of the Biotechnology Program in San Francisco, California, and Peggy Skinner of the BushSchool in Seattle, Washington.

6 We d like to thank them for their invaluable guidance andcontributions to this explorer TeamBio-Rad Life Science Group6000 James Watson DriveHercules, California context. Reinforce learning. Stay scientific discoveries and technologiescreate more content for you to teach,but not more time. BiotechnologyExplorer kits help you teach moreeffectively by integrating multiplecore content subjects into a single lab. Connect conceptswith techniques and putthem into context withreal-world HealthScience Epidemiology and disease Genetic testing Role, place, limits.

7 Andpossibilities of science andtechnology Privacy of information issues Cell structure and organization Tissue types for biologicalsampling Plasmid mapping Use of restriction enzymes andelectrophoresis to fingerprint DNA Use of experimental controls Interpretation of experimental results Use of DNA evidence in court Creation and use of standard curves Restriction fragment lengthpolymorphisms (RFLP analysis) Genetic engineering techniques Mendelian inheritance Plasmid mapping DNA structure, function,and chemistry Chemistry of DNAelectrophoresis Biochemistry ofrestriction enzymes Genetic variations in thehuman genome Genotype vs.

8 Phenotype Biodiversity Bacterial defensemechanismsScientificInquiryChemis tryof LifeGeneticsCell andMolecularBiologyEvolutionTable of ContentsOverviewKit Inventory ChecklistKit Components and Required s ManualInstructor s Manual BackgroundSetting the Stage for Your s Advance Preparation GuideImplementation TimelineAdvance Preparation and Student ChecklistStudent and Instructor Lab s Advance PreparationLab Prep and Lesson GuideGraphic Laboratory ManualPre-Lab ActivityIntroduction to DNA 1 Restriction Digestion of DNA 2 Agarose Gel Electrophoresis of DNA ActivityAnalysis and Interpretation of Activity 1 Plasmid Activity 2 Constructing a AAlternative DNA Fingerprinting BPrelab Review of Restriction Review of CInstructor s Answer DFast Gel EDigital Imaging and Analysis of FPlasmid for the InstructorIntended AudienceThis investigation is intended for use by any high school or college student.

9 Independent of the degree of prior familiarity with the chemistry of nucleic ObjectivesThat all students who participate in this investigation: 1) Become challenged by the task and intrigued by the methodology of the ) Develop an understanding of some of the basic scientific principles involved in DNA fingerprinting and analysis of plasmid ) Weigh evidence and be able to analyze and interpret the data that are generated in thisinvestigation with clarity and ) Have a clear understanding of the thought processes involved in scientific ) Develop the curiosity and confidence to further explore questions and issues involving scientific )

10 Use critical thinking to solve StrategiesThis curriculum is designed to simulate human Forensic testing, but can also be used to simulate a wide range of applications for genetic analysis. The actual scenario employed isup to the discretion of the instructor. Refer to alternative scenarios in Appendix analysis sections of this investigation are intended to guide students through the process of discovering and understanding concepts that are of significance to the proceduresand the analysis of the data at each step along the way.