BLOOD SAMPLE COLLECTION GUIDE.

1 Northern Devon Healthcare NHS TrustPathology Information(Q-Pulse No. PATH-11 Version )7 SAMPLE COLLECTION GuidePage 1 of 7 VersionQ-Pulse NoAuthorised byAuthorCopyLAB = Laboratory performing the test:BBiochemistry (GreenText), Tel 01271 (32)2345 HHaematology (Red Text), Tel 01271 (32)2329 MMicrobiology (Blue Text), Tel 01271 (32)2347 BLOOD Tubes:GGold top - Gel mLPPurple top - EDTA 4 mLGrGrey top - Sodium Fl & EDTA 4 mLBBlue top - Trisodium Citrate mLGnGreen top - Lithium Heparin 4 mLPKPink top - EDTA 6 mLTnTan top - non-gel 4mLDbDark Blue - Sodium heparin 6 mLBlack top - Trisodium Citrate 5 mLContact laboratoryNote: where multiple tubes are purple top tubes will be requiredDocument Key4.

2 Phlebotomy CTOPMore than one test (up to 4) can be combined in one tube provided they are for the same laboratory. Tests analysed in different labs must each have the required SAMPLE COLLECTION of ,STSPECIAL1. Q Pulse3. Phlebotomy C2. Pathology Handbook (on Bob)Laboratory ProcedureReview Period: 3 yearlyNorthern Devon Healthcare NHS TrustPathology Information(Q-Pulse No. PATH-11 Version )7 SAMPLE COLLECTION GuidePage 2 of 7 LABTESTSPECIAL REQUIREMENTSBACEGBACETAMINOPHENGBACR (ACETYLCHOLINE RECEPTOR)GBACTHPPSTRAIGHT TO LABMACTINOMYCESGBADILUMUMAB LEVEL & ANTIBODIESGBAFPGBAIPGBALBUMINGBALDOSTERO NEPPSTRAIGHT TO LABBALCOHOLGrBALLERGY TESTING (RAST)GBALPGBALP ISOENZYMESGBALTGBALUMINIUMDbBAMAGGBAMH (ANTI MULLERIAN HORMONE)GMAMIKACINGBAMINO ACIDSGBAMINOPHYLLINEGBAMMONIAPSTRAIGHT TO LAB, INFORM ANTIBODIESGBAMYLASEGBAMYLOID AGBANA (ANF)GBANCAGBANDROGENSGBANDROSTENEDIONEG BANNA-1 GBANTENATAL DOWN'S SCREENGBANTIBODY (AUTOANTIBODIES)

3 GHANTIBODY ANTENATAL SCREENPKBANTI-CARDIOLIPINGBANTI-CCPGMANT I-DNASE BGBANTI-dsDNAGMANTI-STAPH TITRESGBA1-ANTITRYPSINGHANTI-X ACTIVITYBSTRAIGHT TO LABBAQUAPORIN 4 GHAPTTBMASOTGMASPERGILLUS PRECIPITINSGBASPERGILLUS (ALLERGY)GBASPIRINGBASTGBB12 GHBCR/ABLPPPBEFORE 14:00HR STRAIGHT TO LABBBETA2 GLYCOPROTEINGBBETA2 MICROGLOBULINGBBHCGGBBICARBONATEGBBILE ACIDGTUBESL aboratory ProcedureReview Period: 3 yearlyNorthern Devon Healthcare NHS TrustPathology Information(Q-Pulse No. PATH-11 Version )7 SAMPLE COLLECTION GuidePage 3 of 7 LABTESTSPECIAL REQUIREMENTSTUBESBBILIRUBINGBBIOTINIDASE PorGnMBLASTOMYCESGMBORRELIA BURGDORFERIGMBRUCELLA ANTIBODIESGBBNP (proNT-BNP)GBCA-125 GBCA-153 GBCA-199 GBCAERULOPLASMINGBCALCITONINGON ICE STRAIGHT TO LABBCALCIUMGMCAMPYLOBACTER ANTIBODIESGBCARBAMAZEPINEGTIME OF LAST DOSE REQUIREDBCARBOXYHAEMOGLOBIN/ CARBON MONOXIDEP OR BLOOD GAS SAMPLE , Straight to labBCARDIAC ENZYMESGHCLLPHCD20 PHCD4 Pby 12.

4 00 on FridaysBCEAGBC1 ESTERASEGMCHICKEN POX ( or )GMCHLAMYDIA ANTIBODIESGMCHLORAMPHENICOLGBCHLORIDEGBC HOLESTEROLGBCHOLINESTERASEGBCHROMIUMDbBC HROMOGRANIN A + BPPSTRAIGHT TO LABBCHROMOSOME GnGnSPECIAL FORMBCKGHCOAGULATIONBMCOCCIDIODESGHCLOZA PINE MONITORINGPif local testing requiredBCOBALTDbHCOLD AGGLUTINSGAT 37'C TRANSFUSION LABBCOMPLEMENT (C3,C4)GHCOOMBS TESTPBCOPPERGBCORTISOLGMCOXIELLA ANTIBODIESGBC-PEPTIDEPSTRAIGHT TO LAB, Separate FBC if OF LAST DOSE REQUIREDMCMV ANTIBODIESGMCMV PCRPPHCROSS MATCHPKLABEL BY HAND- SIGNEDBCREATININEGBCRYOGLOBULINTnPKEEP AT 37 C IN FLASK, PRHEATED TUBES TO LAB ASAPBCRPGMCRYPTOCOCCUSGBCYTOGENETICSGnGn SPECIAL FORMHDAT (Direct AntiGlobulin test)PHD-DIMERBMDENGUEGBDEXAMETHASONE ( )GLaboratory ProcedureReview Period: 3 yearlyNorthern Devon Healthcare NHS TrustPathology Information(Q-Pulse No.)

5 PATH-11 Version )7 SAMPLE COLLECTION GuidePage 4 of 7 LABTESTSPECIAL REQUIREMENTSTUBESBDHEASGBDIGOXINGTIME OF LAST DOSE REQUIREDMDIPHTHERIAGHDIRECT COOMBS (DAT)PBDNA (MOLECULAR GENETICS)PPPSPECIAL FORMHDRVT (LUPUS ANTICOAGULANT)BBSTRAIGHT TO LABBDS-DNA (ANTIBODY)GMEBVGMEBV PCRPPBEGFRGBELECTROPHORESIS (PROTEIN)GBELECTROLYTESGBENAGBERYTHROPOI ETINPP rocess ASAP, Separate SAMPLE for FBCHESRTNF, TA/PMR ONLYBETHANOLGrHFACTOR V LEIDENBPBBHFACTOR VIIIBBSTRAIGHT TO LABHFACTOR Xa (Heparin Therapy)BSTRAIGHT TO LABMFARMERS LUNGGBFAECAL REDUCING SUBSTANCESGHFDP (=D-DIMER)BBFERRITINGBFLECAINIDEGEL FREE TUBEHFIBRINOGENBBFOLATE (FOLIC ACID)GHFLOW CYTOMETRYPBFREE ANDROGEN INDEXGBFREE LIGHT CHAINSGBFRUCTOSAMINEGBFSH + LHGBfT4 (free T4)GBfT3 (free T3)GBGADGMGALACTOMANNANGSEE ASPERGILLUSBGALACTOSIDASEPSEE LYSOSOMAL ENZYMESBGAMMA (G) GTGBGASTRINPPSTRAIGHT TO LABBGENTAMICINGTIME OF LAST DOSE REQUIREDHGLANDULAR FEVERPBGLUCOSEGrHGLUCOSE-6-PDPHGLYCATED HBPHGROUP AND SAVEPKLABEL BY HAND AND SIGNBGROWTH HORMONEGBGUT HORMONESPPSTRAIGHT TO LABHHBAICPBHDL-CHOLESTEROLGMHEP A, HEP B, HEP CGMHEP B PCR (VIRAL LOAD)PPMHEP C GENOTYPINGPPPMHEP C RNA/ PCRPPHHFE (DNA)PPPS eparate purple if ProcedureReview Period.

6 3 yearlyNorthern Devon Healthcare NHS TrustPathology Information(Q-Pulse No. PATH-11 Version )7 SAMPLE COLLECTION GuidePage 5 of 7 LABTESTSPECIAL REQUIREMENTSTUBESMHIV VIRAL LOADPMHIV GENOTYPIC RESISTANCEPMHISTOPLASMAGHHLA B27 PSeparate purple if FBC B51 PPTRANSFUSION LABHHLA CLASS 1 + 2 PPPTRANSFUSION LABHHLA NARCOLEPSYPPPBHOMOCYSTINEP STRAIGHT TO LABBHUMIRA/ ADILUMUMABGLEVEL & ANTIBODYMHYDATIDGBIGEGBIGF-1 GBIGF-BP3 GMIMMUNODEFICIENCY SCREENGBIMMUNOFIXATIONGBIMMUNOGLOBULINSG HIMMUNOPHENOTYPINGPHINRBBINSULINPSTRAIGH T TO LABHINTRINSIC FACTORGBIRONGHJAK 2 PAT LAB BEFORE 14:00 HRBKARYOTYPEGnGnSPECIAL FORMBKETONESGBLACTATEGrSTRAIGHT TO LABBLEADPBLDHGMLEGIONELLAGMLEPTOSPIRAGBL FT'SGBLH + FSHGBLITHIUMGTIME OF LAST DOSE REQUIREDHLUPUS ANTICOAGULANTBBMLYME SEROLOGYGBLYSOSOMAL ENZYMESPURINEG aucher/Fabry 1 x Purple 1x Green.

7 Mon - Wed onlyBLUPUS (SLE)GBMAGNESIUMGBMAST CELL TRYPTASEGCONTACT BIOCHEM BEFORE TAKINGMMEASLESGMMENINGOCOCCAL PCRPBMETANEPHRINES (PLASMA)PPSTRAIGHT TO LABHMONONUCLEOSIS / MONOSPOTPMMUMPSGMMYCOPLASMAGBMYELOMA SCREENGBNT- proBNPGMNEEDLESTICKGMNEEDLESTICK INJURY FOLLOW UPPGMNOCARDIAGBOESTRADIOLGBOLIGOCLONAL BANDSGSERUM TO MATCH CSF SAMPLEBORNITHINE GPART OF AMINO ACIDSBOSMOLALITYGBPARACETAMOLGBPARAPROTE IN STUDIESGL aboratory ProcedureReview Period: 3 yearlyNorthern Devon Healthcare NHS TrustPathology Information(Q-Pulse No. PATH-11 Version )7 SAMPLE COLLECTION GuidePage 6 of 7 LABTESTSPECIAL REQUIREMENTSTUBESBPARATHORMONE (PTH)GMPARVO VIRUSGMPARVO VIRUS PCRPPHPAUL BUNNELLPMPERTUSSISGBPHENOBARBITONEGTIME OF LAST DOSE REQUIREDBPHENYTOINGTIME OF LAST DOSE REQUIREDBPHOSPHATEGMPNEUMOCOCCAL ANTIBODYGHPNHPMPOLYOMA VIRUSGBPOTASSIUM (K)GBPROCOLLAGEN III PEPTIDETnGEL FREE TUBEBPROGESTERONEGBPROLACTINGBPROTEINGBP SAGMPSEUDOMONAS ANTIBODIESGHPYRUVATE KINASE (RED CELL)PMQ FEVERGMQUANTIFERONQUANTIFERON GOLD FROM MICROMRABIESGBRAST (SPECIFIC IGE)GBRENINPPSTRAIGHT TO LABMRESPIRATORY SCREENGHRETICULOCYTESPBRF (Rheumatoid Factor)

8 GMRUBELLAGBSALICYLATEGMSCHISTOSOMAL ANTIBODIESGBSELENIUMDbBSHBGGBSIROLIMUSPT IME OF LAST DOSE REQUIREDBSODIUM (Na)GBSPERM AllergyGMSYPHILISGBTACROLIMUSPTIME OF LAST DOSE REQUIREDMTEICOPLANINGHT CELL SUBSETSPCONTACT HAEM BEFORE TAKINGBTESTOSTERONEGMTETANUSGHTHALASSAEM IA TRAIT SCREENPBTHEOPHYLLINEGTIME OF LAST DOSE REQUIREDHTHICK/THIN FILMPHTHROMBOPHILIA SCREEN BBBPGSTRAIGHT TO LABBTHYROGLOBULINGBTHYROID ANTIBODIESGBTIBCGMTOXOCARAGMTOXOPLASMAGB TPMTPS eparate purple if FBC neededBTRANSFERRINGBTRIGLYCERIDEGBTROPON INGBTSHGSPECIALbLaboratory ProcedureReview Period: 3 yearlyNorthern Devon Healthcare NHS TrustPathology Information(Q-Pulse No.)

9 PATH-11 Version )7 SAMPLE COLLECTION GuidePage 7 of 7 LABTESTSPECIAL REQUIREMENTSTUBESBURATE (URIC ACID)GBUREAGBVALPROATEGTIME OF LAST DOSE REQUIREDBVANCOMYCINGTIME OF LAST DOSE REQUIREDMVARICELLA ZOSTER (VZ)GMVDRLGHVISCOSITYPBVITAMIN A + EGKEEP IN DARKBVITAMIN B12 GBVITAMIN DGBVERY LONG CHAIN FATTY ACIDSGHVON WILLEBRANDSBBSTRAIGHT TO LABBVOLTAGE GATED CA. CHANNEL ABSGBVOLTAGE GATED POT. CHANNEL ABSGHWBC + DIFFERENTIALPBWHITE CELL ENZYMESPMON, TUES, OR WED MORNINGS MYELLOW FEVERGMYERSINIAGBZINC TRANSPORTER 8 ABGTRI-PANEL TEST WITH IA2 & AGADBZINCDbLaboratory ProcedureReview Period: 3 yearly