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Cell Lysate Preparation - Biomol
Cell Lysate Preparation RIPA Method Materials: ... Aspirate or decant media; keep cells on ice for all steps. 4. Wash pellet one time with 5 to 10 ml ice cold PBS. 5. Spin 300 x g for 5 minutes. Decant the PBS wash and aspirate the excess supernatant. 6. Add 10 to 100 µl of RIPA Lysis Buffer with Inhibitors per 1 x 106 cells.
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