Column Selection uide - 株式会社ワイエムシィ

1 Column Selection Guide01 USP ---------------------------------------- ------------12 Column Selection guide (Biochromatography) -----------------------------13 Comparison of separation mode --------14~16 Reversed-phase separation of peptides and proteins -----------------------------------17~20 Column Selection guide (Low molecular weight organic compounds) ----21 Application 1 (Fat-soluble vitamins, Water-soluble vitamins) -22 Application 2 (Water-soluble vitamins, Organic acids, Amino acids) --23 Reversed-phase Column Selection guide ---24 Column Selection GuideColumn Selection DescriptionFunctional groupYMC productpageL1 Octadecyl silane chemically bonded to porous or nonporous silica or ceramic microparticles, to 10 m in diameter.

2 Or a monolithic silica C1859~61 YMC-Triart C18 ExRS62 Meteoric Core C1872~75 Meteoric Core C18 BIOYMC-UltraHT Pro C1883 YMC-UltraHT Hydrosphere C1883 YMC-Pack Pro C1884 Hydrosphere C1885 YMC-Pack Pro C18 RS86 YMC-Pack ODS-A87 YMC-Pack ODS-AM87 YMC-Pack ODS-AQ88 YMC-Pack ODS-AL88J'sphere ODS-H8089J'sphere ODS-M80J'sphere ODS-L80L3 Porous silica particles, to 10 m in diameter, or a monolithic silica SIL104 YMC-Pack SIL-06L7 Octylsilane chemically bonded to totally porous or superficially porous silica particles, to 10 m in diameter, or a monolithic silica C863 Meteoric Core C872~75 YMC-Pack Pro C896 YMC-Pack C897 YMCbasic100L8An essentially monomolecular layer of aminopropylsilane chemically bonded to totally porous silica gel support, to 10 m in NH2108L10 Nitrile groups chemically bonded to porous silica particles, to 10 m in CN99L 11 Phenyl groups chemically bonded to porous silica particles, to 10 m in Phenyl64 YMC-Pack Ph98L13 Trimethylsilane chemically bonded to porous silica particles.

3 3 to 10 m in TMS98L20 Dihydroxypropane groups chemically bonded to porous silica or hybrid particles, to 10 m in Diol-HILIC66 YMC-Pack Diol-NP104 YMC-Pack Diol-6045, 46 YMC-Pack Diol-120 YMC-Pack Diol-200 YMC-Pack Diol-300L24 Polyvinylalcohol chemically bonded to porous silica particle, 5 m in PVA-Sil105L26 Butyl silane chemically bonded to totally porous silica particles, to 10 m in Pro C496 YMC-Pack C497 YMC-Pack PROTEIN-RP99L27 Porous silica particles, 30 to 50 m in SIL-HG130, 135L33 Packing having the capacity to separate dextrans by molecular size over a range of 4,000 to 500,000 Da.

4 It is spherical, silica-based, and processed to provide pH Diol-6045, 46 YMC-Pack Diol-120 YMC-Pack Diol-200 YMC-Pack Diol-300L40 Cellulose tris-3,5-dimethylphenylcarbamate coated porous silica particles, 5 to 20 m in tris-3,5- dimethylphenylcarbamateCHIRAL ART Cellulose-C26~29L43 Pentafluorophenyl groups chemically bonded to silica particles by a propyl spacer, to 10 m in PFP65L51 Amylose tris-3,5-dimethylphenylcarbamate-coated, porous, spherical, silica partilces, 5 to 10 m in tris-3,5- dimethylphenylcarbamateCHIRAL ART Amylose-C26~29L59 Packing for the size-exclusion separation of proteins (separation by molecular weight) over the range of 5 to 7,000 kDa.

5 The packing is a spherical 10- m, silica or hybrid packing with a hydrophilic Diol-6045, 46 YMC-Pack Diol-120 YMC-Pack Diol-200 YMC-Pack Diol-300L62C30 silane bonded phase on a fully porous spherical silica, 3 to 15 m in Carotenoid100 Column Selection Guide13 Column Selection guide (Biochromatography)For separatiion or molecular weight determination of sugarsFor separation of polar compoundsSize exclusionHILICS ugarsYMC-Pack DiolYMC-Triart Diol-HILICYMC-Pack Polyamine IIFor separation of biomolecules by the difference in surface chargeFor separation of polar compounds with poor retention on reversed-phase columnsIon exchangeHILICP roteinsPeptidesYMC-BioProSize exclusionYMC-Pack DiolYMC-Triart Diol-HILICS uitable as the first choice ODS columnFor separation of biomolecules by molecular weightFor separation of biomolecules with molecular weight

6 Of up to 30,000 using high temperatureCore-shell Column for separation of biomolecules with molecular weight of up to 30,000 Column with wide pore size useful for separation of macromoleculesSpecialized Column with excellent acid resistance for separation of proteins or peptidesMolecular weight5,000 or lessMolecular weight5,000 or moreReversed-phaseYMC-Triart C18 YMC-Triart C18 Meteoric Core C18 BIOWide-Pore ColumnsYMC-Pack PROTEIN-RPFor separation of biomolecules by the difference in surface chargeFor separation of polar compoundsIon exchangeHILICYMC-BioProSize exclusionYMC-Pack DiolYMC-Triart Diol-HILICYMC-Pack Polyamine IIUsable with 100% aqueous mobile phaseFor separation of biomolecules by molecular weightUsable with 100% aqueous mobile phaseColumn with wide pore size useful for separation of macromoleculesNucleic acid basesNucleosidesNucleotidesNucleic acid

7 BasesNucleosidesNucleotidesOligonucleoti desOligonucleotidesNucleic acidsOligonucleotidesNucleic acidsReversed-phaseYMC-Triart C18 Hydrosphere C18 YMC-Triart C18 Hydrosphere C18 Wide-Pore ColumnsUsable with 100% aqueous mobile ~ ~47 Meteoric Core C18 Core-shell Column with ultra fast ~ ~ ~ ~ ~ ~ , ~ ~ , , ~ ~ , , C18 Hydrosphere C18 Nucleic acidsColumn Selection GuideColumn Selection Guide1401 Comparison of separation modeSeparation of proteins by different modeHuman serumMouse monoclonal IgG1 anti-human IgG4 (Purified by DEAE chromatography, containing NaN3)Mouse IgG Fc fragment (Prepared from normal serum)Proteins in human serum are separated by the difference in the surface charge on ion exchange chromatography (IEC) and by the difference in the molecular weight on size exclusion chromatography (SEC).

8 Mouse monoclonal antibody against human IgG4 is analyzed on ion exchange chromatography (IEC) and size exclusion chromatography (SEC). Several peaks possibly derived from isoform of antibody are observed in ion exchange mode, while a single peak is detected in size exclusion exclusion chromatography (SEC) is useful for separation of substances which have distinct differences in molecular weight, like between IgG and its fragments. On the other hand, reversed-phase chromatography (RPC) is suitable for a precise analysis of peptides and proteins with a molecular weight of less than 100 kDa such as IgG Fc minTransferrinAlbuminN080313 EIgGmAU100 Ion exchangemin2468101214160 NaN3 Mouse monoclonal IgG1(Anti-human IgG4)mAU012345 P080220 AIon exchange1.

9 Rabbit IgG2. Mouse IgG Fc fragmentminmAU0510152002550R080623D12 Size exclusion0102030405060 minN080616 AIgGAlbuminTransferrinmAU150100050 Size exclusion010515202530 minNaN3 Mouse monoclonal IgG1(Anti-human IgG4)010203040mAUP080530 ASize exclusion3040352520151050minR080619 BMouse IgG Fc fragmentmAU0102030 Reversed-phaseYMC-BioPro QA 5 m, 50 X QA-F 5 m, 30 X Diol-200 5 m, 300 X Diol-300 + Diol-200 5 m, 300 X X 2 YMC-Pack Diol-200 5 m, 300 X C4 (300 ) 5 m, 150 X : A) 20 mM Tris-HCl (pH ) B) 20 mM Tris-HCl (pH ) containing M NaCl 0-30%B (0-15 min), 30-100%B (15-30 min)Flow rate : mL/minTemperature : 25 CDetection : UV at 280 nmInjection : 20 L (100 L/mL)Eluent : A) 20 mM Tris-HCl (pH ) B) 20 mM Tris-HCl (pH ) containing M NaCl 10-25%B (0-18 min)Flow rate : mL/minTemperature : 25 CDetection : UV at 220 nmInjection : 10 L ( mg/mL)Eluent : M KH2PO4-K2 HPO4 (pH ) containing M NaClFlow rate : mL/minTemperature : ambient (27 C)Detection : UV at 220 nmInjection.

10 5 L ( mg/mL)Eluent : M KH2PO4-K2 HPO4 (pH ) containing M NaClFlow rate : mL/minTemperature : ambient (25 C)Detection : UV at 280 nmInjection : 20 L (100 L/mL)Eluent : M KH2PO4-K2 HPO4 (pH )Flow rate : mL/minTemperature : ambient (25 C)Detection : UV at 220 nmInjection : 10 L ( mg/mL)Eluent : A) water/TFA (100 ) B) acetonitrile/TFA (100 ) 25-45%B (0-40 min)Flow rate : mL/minTemperature : 37 CDetection : UV at 220 nmInjection : 5 L ( mg/mL) Column Selection Guide15060 min2010304050N080422B*01020mAUIon exchange060 min201030405002030mAU1040N080318B*Revers ed-phase060 min201030405003050mAU60402010N080623L105 102103104MW54321101*Size exclusionSeparation of sugar chains by different modeSeparation of proteins by different modeYMC-BioPro QA 5 m, 50 X 5 m, 150 X Diol-120 + Diol-60 5 m, 500 X X 2