Dionex ICS-1100 Ion Chromatography Operator's Manual

1 Dionex ICS-1100 Ion Chromatography System Operator s ManualDocument No. 065289 Revision 03 October 2012 2012 by Thermo Fisher Scientific Inc. All rights , AMMS ICE, ASRS, Chromeleon, Dionex , IonPac, OnGuard, and SRS are registered trademarks of Thermo Fisher Scientific Inc. in the United , Windows, Windows 2000, and Windows XP are registered trademarks of Microsoft Corporation in the United States and other countries. Adobe, Acrobat, and Adobe Reader are registered trademarks of Adobe Systems, Incorporated in the United States and other and RFIC are trademarks of Thermo Fisher Scientific is a trademark of Victrex other trademarks are the property of Thermo Fisher Scientific Inc.

2 And its Fisher Scientific Inc. provides this document to its customers with a product purchase to use in the product operation. This document is copyright protected and any reproduction of the whole or any part of this document is strictly prohibited, except with the written authorization of Thermo Fisher Scientific contents of this document are subject to change without notice. All technical information in this document is for reference purposes only. System configurations and specifications in this document supersede all previous information received by the purchaser. Thermo Fisher Scientific Inc. makes no representations that this document is complete, accurate or error-free and assumes no responsibility and will not be liable for any errors, omissions, damage or loss that might result from any use of this document, even if the information in the document is followed properly.

3 This document is not part of any sales contract between Thermo Fisher Scientific Inc. and a purchaser. This document shall in no way govern or modify any Terms and Conditions of Sale, which Terms and Conditions of Sale shall govern all conflicting information between the two history: Revision 01 released March 2009; initial release of systemRevision 02 released December 2011; Thermo Fisher Scientific transitionRevision 03 released October 2012; new part numbers assignedFor Research Use Only. Not for use in diagnostic 065289-03 10/12i1 Introduction.. to Ion Chromatography (IC) .. of the Dionex ICS-1100 .. This Manual .. Messages and Notes.

4 And Regulatory Information .. Labels .. 82 Features.. Features .. Panel .. Cover .. Panel .. Panel .. Panel .. Schematics .. and Chromeleon Xpress .. Panel Tabset .. Control Modes .. Wellness .. 25 ContentsDionex ICS-1100 Ion Chromatography SystemiiDoc. 065289-03 10 Component Details .. Degas Assembly (Optional) .. Valve .. Valve .. Valve (Optional) .. Heater (Optional) .. Heated Conductivity Cell .. Controller ..353 Operation and Maintenance .. Overview .. On the System Power .. to Chromeleon .. Up the Eluent Reservoir .. the Eluent .. the Eluent.

5 The Eluent .. the Reservoir .. the Eluent Level .. the Reservoir .. All Connections .. the Pump ..45 ContentsDoc. 065289-03 10 System Operating Conditions .. the System and Verify Operational Status .. Samples .. and Storing Samples .. Samples .. Samples .. Loading and Injecting Samples .. Loading Samples with a Syringe .. Loading Samples with a Vacuum Syringe .. Loading Samples with an Autosampler .. Injecting Samples .. Processing Samples .. Manual Sample Processing .. Automatic (Batch) Sample Processing .. Maintenance .. 554 Troubleshooting.

6 Messages .. Error Messages .. Leaks .. Difficult to Prime or Loses Prime .. Does Not Start .. Flow .. Flow/Pressure Reading .. 73 Dionex ICS-1100 Ion Chromatography SystemivDoc. 065289-03 10 System Backpressure .. Ghosting .. Nonreproducible Peak Height or Retention Time .. Abnormal Retention Time or Selectivity .. No Cell Response .. High Cell Output .. Baseline Noise or Drift .. Vacuum Degas Assembly Does Not Run ..795 Service .. and Calibration Procedures .. Wellness Panel Overview .. the Conductivity Cell .. the Flow Rate .. the Vacuum Degas Assembly .. a Restriction in the Liquid Lines.

7 Tubing and Fittings .. the Injection Valve or Auxiliary Valve .. an Auxiliary Valve Pod .. and Replacing the Pump Check Valves .. a Pump Piston Seal and Piston Rinse Seal .. a Pump Piston .. the Waste Valve or Priming Valve O-Ring .. Replacing the Conductivity Cell ..104 ContentsDoc. 065289-03 10 Replacing the Suppressor .. Replacing the Column Heater .. Replacing the Column Heater Heat Exchanger .. Replacing the Eluent Valve .. Replacing the Leak Sensor .. Priming the Pump .. Priming the Eluent Lines with a Syringe .. Priming with the Prime Button .. Priming the Pump with Isopropyl Alcohol.

8 Changing Main Power Fuses .. 117A Specifications.. Panel .. Pump and Fluidics .. Regeneration .. Electronics .. Cell with Heat Exchanger .. Valve .. Auxiliary Valve (Optional) .. Vacuum Degas Assembly (Optional) .. Column Heater (Optional) .. 124 Dionex ICS-1100 Ion Chromatography SystemviDoc. 065289-03 10 Suppressors .. Autosampler .. System Software ..126B TTL and Relay Control.. and Relay Connections .. TTL Input Functions and Control Types .. TTL and Relay Outputs ..134C Reordering Information..135D FAQ.. do I hook up an autosampler? .. do I print? .. are the retention times moving?

9 Do I adjust retention times? .. should I remake standards? .. should I remake eluents? .. do I start Chromeleon? .. do I delete data? .. do I back up data? .. How do I shut off the system? .. How do I store columns? .. How do I know when a column is dirty? ..140 ContentsDoc. 065289-03 10 How do I clean a column? .. Why is the conductivity high? .. How do I configure and operate the auxiliary valve? .. 141E Glossary .. 143 IndexDionex ICS-1100 Ion Chromatography SystemviiiDoc. 065289-03 10/12 Doc. 065289-03 10/1211 to Ion Chromatography (IC)The Thermo Scientific Dionex ICS-1100 Ion Chromatography System ( Dionex ICS-1100 ) performs ion analyses using suppressed or non-suppressed conductivity detection.

10 An ion Chromatography system typically consists of a liquid eluent, a high-pressure pump, a sample injector, a guard and separator column, a chemical suppressor, a conductivity cell, and a data collection running a sample, the ion Chromatography system is calibrated using a standard solution. By comparing the data obtained from a sample to that obtained from the known standard, sample ions can be identified and quantitated. The data collection system, typically a computer running Chromatography software, produces a chromatogram (a plot of the detector output vs. time). The Chromatography software converts each peak in the chromatogram to a sample concentration and produces a printout of the results.