細胞の増殖とDNA複製 - seika.kpu.ac.jp

1 DNA M S G G G WT1 RBp53 RasMycFosJun 5'5'3'3'5'5'3'3' 5' 3' RNA 5'5'5'5'5' 5'5'3'3'3'5'5'3' DNA ATP DNA DNA 1 DNA SSB) RNA RNA 4-15 DNA RNA 3 5 3 DNA RNA 3 5 3 DNA DNA DNA 14 28 42 56 70040302010040 30 20 Proliferative lifespan 1 PDL Population doubling level=PDL 2 PDL 3 PDL

2 100 G0 7550250 PDL0- 1010- 2020- 3030- 4040- 5050- 6060- 7070- 8080- 90 Martin 1970 Progeria Warner Syndrome 7550250 PDL0 10 20304050607080 Goldstein,1978 telomere 5'-TTAGGGTTAGGGTTAGGG-----3' repeated sequence TTAGGG n. 2,000 5'5'3'3'5'5'3'3' 5' 3' RNA 5'5'5'5'5' 5'5'3'3'3'5'5'3' 1930 B.

3 McClintock Muller Muller : (telomere) 1972 Blackburn ( Tetrahymena) DNA 1972 Watson 1984 Greider 1973 Olovnikov 1050 kbp(Allsopp 1992)57911204060 kb)0-25 26-51 51-76 1988 Greider 1990 Hastie 1992 Counter TAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTT telomerase TAGGGTTAGGGTTAGGGTTAGG-3'ATCC-5'AATCCCAA TTAGGGTTAGGGTTAGGGTTAGGGTTAAATCCCAATTAGG GTTAGGGTTAGGGTTAATCC-5'CCCAATCCCAATCCCAA TCCCAAT-5'ATCC-5'ATCCDNA (Blackburn 1991)

4 Nakamura (1997)Meyerson (1997)Kilian (1997) Weinrich (1997) (1998) Bordnar (1998) 20 54 74 64 100 Shiels (1999) Nature May 27 399 :(316-317)Analysis of telomere lengths in cloned sheep _ PCR PCR (Polymerase Chain Reaction) Kary Mullis 1986 DNA DNA 1993 Mullis 5 -atttactccagccatgctaagaaagctacc-3 3 -taaatgaggtcggtacgattctttcgatgg-5 5 -atttactccagccatgctaagaaagctacc-3 3 -taaatgaggtcggtacgattctttcgatgg-5 94 C 95 C A-T G-C A-T ATGC5 -atttactccagccatgctaagaaagctacc-3 3 -taaatgaggtcggtacgattctttcgatgg-5 DNA ctttcgatgg5 5 atttactcca AT-GC 55 C Taq DNA A GCT dNTP 72 C C 10

5 DNA Taq DNA 2 4 225 33,554,432 3000