遺伝子の転写と翻訳 - seika.kpu.ac.jp

1 53535353 RNA 5 ----TTGACA---------TATAAT----35-1016-19 bp5-9 bp5 ---- CAAT------TATAAT-----------CAAT Box25 bp RNA polymeraseGenomic DNAmRNATATA Box mRNA 5353 RNA 5353 5 mRNA 53 5 AUGAAAAAAAAAAGppp 5 A Gppp---AATAAA-----(10-30 upstream)(100-200) (The cell) TATA mRNA RNA Poly-A AUG -AAAAAUAA G-ppp- 5 5 -atttactcca gccatgctaa gaaagctacc-3 3 -taaatgaggt cggtacgatt ctttcgatgg-5 RNA 3 > 5 mRNA mRNA 5 -auuuacucca gccaugcuaa gaaagcuacc-3 * DNA 1 ggccgatata tatcagtgcc tgatttgaga ttgggtccca ggtctcggtg gaatcggga61 cgtggtgatt gtgggtgggg gctttggcgg gatcgcagca gccagccagc tgcaggccct121 gaacgtcccc ttcatgctgg tggacatgaa ggactccttc caccacaatg tggctgctct181 ccgagcctcc gtggagacag ggttcgccaa aaagacattc atttcttact cggtgacttt241 caaggacaac ttccggcagg ggctagtagt ggggatagac ctgaagaacc agatggtgct301 gctgcagggt ggcgaggccc tgcccttctc tcatgttatc ctggccacgg gcagcactgg361gcccttcccg ggcaagttta

2 Atgaggtttccagccagcag gccgctatcc aggcctatga421ggacatggtg aggcaggtcc agcgctcacg gttcatcgtg gtggtgggag gaggctcggc481tggagtggag atggcagcag agattaaaac agaatatcct gagaaagagg tcactctcat541tcactcccaa gtggccctgg ctgacaagga gctcctgccc tccgtccggc aggaagtgaa601ggagatcctc ctccggaagg gcgtgcagct gctgctgagt gagcgggtga gcaatctgga661ggagctgcct ctcaatgagt atcgagagtacatcaaagtg cagacggaca aaggcacaga721ggtggccacc aacctggtga ttctctgcac cggcatcaag atcaacagct ccgcctaccg781caaagcattt gagagcagactagccagcag tggtgctctg agagtgaacg agcacctcca841ggtggagggc cacagcaacg tctacgccat tggtgactgt gccgacgtga ggacgcccaa901gatggcctat cttgccggcc tccacgccaa catcgccgtg gccaacatcg tcaactctgt961gaagcagcgg cctctccagg cctacaagcc gggtgcactg acgttcctcc tgtccatggg1021gagaaatgac ggtgtgggcc aaatcagtggcttctatgtg ggccggctca tggttcggct1081gaccaagagc cgggacctgt tcgtctctac gagctggaaa accatgaggc agtctccacc1141 ttgatggaga ggccaggcgg gagaactacc gcagcaggtg ggcgtacgga ctgcttggcg1201 catggcaccc gcctggcaag tgctagaact aatgctattc ttctggaata agatgccaat1261 gatgtggtgg ctagaaatgc aacttgtata aaacaaaaat gggagagaga gaggtattaa1321 acaaatacccTGA ATG( TATABOX( M-AAAAAG-ppp-M-AAAAAG-ppp-MAUG tRNA 5 AUG cDNA RNA RNArRNA( mRNA( DNA rRNAmRNADNA RNA28S18 SOligo dT rRNA, mRNArRNA mRNAAAAAAA-3 5 AAAAAA-3 5 3 -TTTTTT-5 TTTTTTT TTTTTT-5 AAAAAA-3 DNA mRNATTTTTT-5 3 RNA TTTTTT-5 DNA TTTTTT-5 AAAAAA-3 cDNA 5 3 (1977)))))

3 RNA 150 Mb (1000 x kb) AAAAAAAAAAAARNA mRNA5 ------- AGGU AGU---------- AG --------3 CAAGU U UUU UUUC C CCC CCCGA5 3 cDNA cDNA (Vector) (Phage) (Plasmid) cDNA cDNA cDNAAAAAAA-3 5 3 -TTTTTT-gattcgaa-5 TTTTTT-gattcgaa-5 AAAAAA-ctaagctt-3 cDNA DNA 150 Mb (1000 x kb) DNA Science 222 11/18 1983 hGH