Evolution 300 Evolution 600 UV-Vis Spectrophotometers

1 Evolution 300 Evolution 600 UV-Vis SpectrophotometersLife ScienceMaterial SciencePharmaceuticalmolecular spectroscopyPart of Thermo Fisher Scientific2 Reliable, High-Performance UV-Visible SpectrophotometersFor over 60 years, customers in world-renowned research institutions, highly regulated QC laboratories and teaching institutions have relied on Thermo Scientific UV-Visible ( UV-Vis ) Spectrophotometers . The Evolution UV-Visible 300/600 spectrophotometer series is founded on the successful legacy of the Pye-Unicam, Bausch & Lomb, Philips Scientific,and Spectronic products. Many customers today say they made their first absorbance measurement on a SPECTRONIC 20 spectrophotometer, a product on the market since1953. The Evolution 300/600 series continues in this tradition by delivering the solid, high-performance you need for your UV-Vis PU8800, designed by Pye Unicam inthe 1980 s, was one of the industry-leadingresearch instruments of its Design4 System Configuration6 Life Science Biochemical 8 Pharmaceutical 10& QC AnalysisMaterial Science12 Smart Accessories14 Thermo Scientific UV-VisSpectrophotometer Product LinesSPECTRONIC 20/20D, SPECTRONIC Educator ,and GENESYS 20 Basic teaching Easy sample and data handlingGENESYS 10 Series Visible, UV-Vis , and UV-Vis scanning options Routine quantitative analysis and common bio assays Easy-to-use accessoriesHelios , Evolution 60.

2 And Evolution 100 Routine quantitative analysis and bio assays More advanced accessories and softwareEvolution 300/600 Series Wide range of accessories Advanced quantitative analysis and bio assays Extensive software offerings Tablet dissolution Material science3 Life ScienceMaterial SciencePharmaceuticalQCBiochemicalThe Evolution 300/600 spectrophotometer seriesincorporates a high-performance optical design, versatileVISION software packages, and the highest qualityaccessories for the most demanding applications. Thesystems offer a multitude of advantages for a wide variety of applications including: Life Science Biochemical Pharmaceutical & QC Material ScienceInnovative Smart Accessories offer ease of use andincreased productivity by providing: Snap-and-go installation Seamless software integration Serial number tracking for the regulated environmentSystem configuration choices offer the ultimate in flexibility for your laboratory.

3 Choose from: Stand-alone Local Control version PC Control version Combination of bothEvolution 300 & Evolution 600 UV-Vis Spectrophotometers High-Performance Optical Design for Reliable ResultsMaintaining high performance is the ongoing legacy you can count onwith the Thermo Scientific Evolution 300/600 spectrophotometer Lamp Primary referencestandard, allowsseamless validationand calibration ofthe instrumentLight SourceXenon flash lamp is only on when taking measurementsextending its lifetimeOptics Quartz overcoatedoptics for long-lasting reflectivity and cleaning withoutdamaging the optical surfaceChassisRigid, cast-aluminum base with opticalmounts bolted directly onto the base providessuperior system stability and reliable resultsDetectors Silicon photodiodes forextended wavelengthrange to 1100 nmMicrometer MonochromatorDrive with Sliding CouplingProvides excellent accuracy, reproducibility and minimizes temperature effectsSlit MechanismPhoto-etched slits for greater driven slit drive with intelligent optimizationGrating 1200 lines/mm, 240 nm blazed, holographic grating, for exceptional stray light performanceEvolution 300 OpticsThe Evolution 300 optical design includes a long lifetime xenon flash lamp and extended, wavelength-range silicon photodiode detectors.

4 This system is an excellent choice for the routineand research laboratories in the Life Science and Pharmaceutical 600 Optics5 Light SourcesDeuterium andHalogen lamps foradded stabilitywhen measuringminute absorbancechangesMonochromatorBoth models featureresearch grade, Ebert monochromatorfor excellent energythroughputSource OpticsAuto-aligningsource optics automatically optimizethroughputwhen the lamp ischangedSmart SamplingPlug-in, instrument-controlled accessoriesfor ease of use and reliabilityDetector High-performance,red-sensitive photomultiplier(PMT) detector fordemanding samplesChopperFour-segment chopper runningat 33 Hz eliminates possibleoscillation conflicts with globalmains AC power frequencies of 60 or 50 HzSample Compartment Large sample compartmentand wide beam separationwith interchangeable baseplates for full sampling versatilityThe optical design of the Evolution 600 includes dual deuterium and tungsten sources and ahigh-performance photomultiplier detector.

5 These features provide enhanced high-levelperformance for the Material Science market and general research 300 & Evolution 600 UV-VisSpectrophotometers Operational Flexibility to Fit Your Lab NeedsThe Evolution 300/600 series offers the flexibility to choose a configuration suited to your needs: Local Control, PC software control,or Local Control with PC software control. Each of these configurationsoffers various levels of operational flexibility to fit the individual ormulti-user laboratory environment. 6 Local ControlThe Local Control configuration offers complete instrument control, comprehensivemethod and data file manipulation, and savesvaluable bench space. It can also be pass-word protected preventing unwanted accessfrom outside your laboratory. For presentationpurposes, the Local Control screen displaycan be viewed on an external monitor orprojection system using the DVI and PC ControlThe Local Control with PC software controlconfiguration gives you the freedom tochoose the configuration that matches yourexperimental needs.

6 For example, use LocalControl to make a quick absorption meas-urement to determine the purity of a DNAsample. You do not need to wait for the PC toboot and the software to load. Later, to runa kinetics experiment based on a methodstored on a network drive, simply openVISION software, and switch the instrumentto PC control. This configuration is ideal forlabs with a variety of experimental ControlThe PC Control configuration allows users tocollect data and distribute it to third-partyapplications for advanced processing andarchival. In multi-user environments, LocalControl with PC software control gives youthe ultimate PC Software Control configurationuses the powerful VISION suite of softwareto control the Evolution 300/600 software allows you to collect, store,recall, and analyze your data, using oneconvenient program. From QC quantitativeanalysis measurements to complex enzymaticassays, the right VISION software packageis available for your large, color display and tactile keypadmake the Evolution Local Control system convenient and easy-to-use.

7 7 Smart Start SmartStart allows each user to set up theSmartStart menu with the applications they usemost often. This increases the throughput of everyuser by eliminating the need to navigate menusand by decreasing the amount of time required totrain users. Load the SmartStart menu with anyapplication, including your stored methods, andwatch the productivity of your lab standard VISIONpro software offers advanced scanning, multiple fixed wavelength measurements,and multi-wavelength measurements with customized UVcalc. Quantitative analysis and multicomponentanalysis applications are also Software SuiteVISIONpro is a flexible software packagedesigned for the general research and QClaboratory. When used with VISIONpro, theVISION life software package adds the abilityto monitor and calculate reaction kineticsand to perform DNA melting those laboratories requiring 21 CFR Part11 compliance, VISION security sets thestandard for UV-Vis application software packages for the Evolution 300/600 UV-Visseries include: VISION Chroma for color measurements and EnzLab for EnzymaticFood Software Application Comparison ChartSoftwareScanningFixed Abs/%TQuantMulticomponentRateDNAVISIONpr o VISIONpro + VISION life VISION security VISION security + VISION life Customized ReportingThe Evolution advanced Report Composer inVISIONpro, VISION life and VISION security software allows you to precisely configure theformat of reports.

8 These templates can be linked toan application method and used to automaticallyproduce reports in your customized Acid TestsThe Evolution series provides all the necessarytests for determining DNA purity in thepresence of protein or phenol. DNA/RNAconcentrations can be determined by simpleratios or by wavelength scanning. Thewavelength scanning option allows theadded flexibility of checking for potentialcontaminations. Various quantization methods are available to determine the concentration ofpurified double-stranded and single-strandedDNA and RNA. Based on user-entered factors,the Evolution instrument measures theabsorbance of the DNA or RNA and calcu-lates the appropriate Oligo (Calc Factor) function allowsyou to enter a specific oligonucleotidesequence and from a subsequent absorbancemeasurement, the system calculates the%GC content, MW of the oligo, molar(pmol/ul) concentration of the sample andan estimate of the 300 & Evolution 600 UV-Vis Spectrophotometers 8 With no external circulating water required, the Evolution spectrophotometerwith the Smart Peltier accessory can reach a maximum temperature of 110 C.

9 This extended range provides added flexibility for making accurate Tmmeasurements on very long oligonucleotides. Protein ConcentrationIf your research involves the determination ofprotein concentration assays, the EvolutionLocal Control systems provide the standardprotein assays at the push of a Denaturation/RenaturationWhether your research examines short orlong DNA or RNA sequences, duplexes ortriplexes, the Evolution VISION lifeThermalDenaturation/Renaturation system will meetall of your experimental needs. The systemprovides all the tools necessary to controlcomplex heating and cooling profiles withuser defined hold times and ramp ratesdown to C/min. Multiple built-in fittingalgorithms are available to calculate the Tmvalue from all types of melting Solutions for Life Science Biochemical ApplicationsAll of the major, industry-standard protein concentration assays at your Evolution 300/600 series is the natural fit for any multi-user, life-scienceresearch laboratory.

10 For the user performing fast or routine assays, such asNucleic Acid tests, to users performing more advanced studies, such asMichaelis-Menton kinetics or Thermal Denaturation/Renaturation assays,these instruments offer a configuration ideal for your the Local Control and PC Control configurationscater to the user performing single or multi-celldouble-beam kinetics. The Local Control colorscreen makes it easy to distinguish the individualresults from multiple kinetics reactions. Up toseven active reference cuvettes can be used in aseven-sample/seven-reference configuration. Forlaboratories performing more complex assays, suchas inhibitor studies, VISION lifesoftware providescalculations for the Michaelis-Menton Kmand Vmaxparameters using methods such as Lineweaver-Burke and of the routine biological assays, including abuilt-in Oligo calculator, are accessible in onlytwo Analysis Made SimpleThe Evolution Quant application is availablein both the Local Control and PC Controlconfigurations.