FlowCAM Manual FINAL SEP11

1 FlowCAM Manual Version September 2011 Fluid Imaging Technologies, Inc. 65 Forest Falls Drive Yarmouth, Maine 04096 USA Tel: Fax: - 2 - - 3 - FlowCAM Standard Factory Limited SECTION The Scope of this SECTION TWO-MODES OF Understanding AutoImage Understanding Trigger Mode (Fluorescence & Scatter)..16 SECTION THREE-INSTRUMENT Instrument Model C50 & C51 Peristaltic Model C70 & C71 Syringe SECTION Main File Edit Analyze Setup Setup Setup Setup and (F6)..72 AutoImage Mode (No Save).. (F7)..77 Trigger Mode (F8)..78 Trigger Mode (No Save).

2 (F9)..78 File Processing Mode (No Save)..78 Tools Preferences Help View Tool Status View File View Edit View Sort View Filter View Statistics View Show Right Click Popup Keyboard - 4 - Library Library File Library Edit Library Sort Library Show Right Click Popup Keyboard Classification Classification File Classification Edit Classification Classes Classification Operation Classification Sort Classification Classification Statistics Classification Show Right Click Popup Keyboard Appendix I: Flow Cells and Tubing Appendix II: Glossary of Particle Properties & Other Appendix III: How VisualSpreadsheet Determines Parts/Million and Appendix IV: FlowCAM Trigger Mode Appendix V: FlowCAM Calibration Verification FlowCAM Size & Count Calibration Verification Appendix VI: FlowCAM Quick Start Appendix VII: Standard FlowCAM Hardware Appendix VIII: Frequently Asked Questions (FAQ).

3 136 - 5 - Thank You Fluid Imaging Technologies, Inc. would like to thank you for making the important decision to purchase the FlowCAM system. We are committed to manufacturing quality products and providing unsurpassed customer service. Copyright Notice FlowCAM and VisualSpreadsheet are registered trademarks of Fluid Imaging Technologies, Inc. Microsoft Windows and Microsoft Excel are trademarks of Microsoft Corporation. All other trademarks used within this Manual are hereby acknowledged. Copyright 2011 Fluid Imaging Technologies, Inc. All Rights Reserved Worldwide. Fluid Imaging Technologies customers may reproduce this Manual in printed form provided the content remains unchanged.

4 Reproduction or republication of any portion of this Manual for any other purpose is prohibited without the expressed written consent of Fluid Imaging Technologies, Inc. Manual Information FlowCAM Manual . Version , September 2011. VisualSpreadsheet , Portable, Open Benchtop, Benchtop Models. Manual edited by Benjamin Spaulding, Laboratory Manager. - 6 - Before operating the system, read the Manual carefully to prevent damage to humans, animals, integrated devices, and connected devices. Always follow local safety rules. Do not look directly into the laser beam. Do not look at any laser light directly or through optical lenses.

5 Wear appropriate safety glasses to prevent laser light from entering the eye, even reflections from any surface. When changing optical components, always disable (via VisualSpreadsheet) the laser. Beware of electrical shock hazard. To prevent the risk of electrical shock or fire, always disconnect the AC/DC power before disconnecting cables or wiring connectors. Electrical units should not be operated in hazardous environments. Do not touch any damaged, non-isolated electrical parts. This instrument may be harmed by mishandling the flow chamber and/or removing the photomultiplier tube from its mounting bracket and/or exposing it to direct room light (while the unit is plugged in and has power).

6 Improper software settings will not cause damage. There are no user serviceable parts inside this unit. Do not attempt any repairs yourself. Doing so will void your Standard Factory Limited Warranty. Should your FlowCAM require service, please contact Fluid Imaging Technologies, Inc. for additional instructions. This product must not be disposed of in normal household waste. Contact Fluid Imaging Technologies for proper disposal instructions. - 7 - Standard Factory Limited Warranty Fluid Imaging Technologies, Inc. ( Seller ) warrants that the FlowCAM product ( Product ) purchased by you ( Customer ) shall be free from material defects in workmanship and material for a period of one (1) year from the date of shipment by the Seller (the Limited Warranty ); provided, however, that the Limited Warranty does not cover any consumables (flow cells, flow cell holders, or tubing) or third party manufactured/customer purchased items incorporated in the Product.

7 For Service and Spare/Replacement parts, the Seller also warrants the services we perform and the spare and replacement parts we install, for a period of one (1) year from the date of performance of such services and the date of installation of the spare or replacement parts, respectively. Customer understands and agrees that the Limited Warranty shall only apply if Customer has used the Product in accordance with all specifications, documentation, and other information provided to Customer by Seller. Customer s sole and exclusive remedy and Seller s entire liability under the Limited Warranty shall be (i) at Seller s option, repair or replacement of the Product or any defective Product components (including any labor or other services related thereto), and (ii) all shipping costs related to the repair or replacement of the Product or any defective Product components for both on-site and off-site repairs and replacements.

8 Any Product or Product components returned to Seller must have prior approval and must reference a Return Material Authorization (RMA) number issued by Seller. In the event Seller determines that the entire Product must be returned to Seller for repair or replacement, such Product must be shipped in its original shipping container to assure adequate protection during transit. If the original shipping container is not available, a new shipping container may be purchased from Fluid Imaging Technologies, Inc. at an additional cost. The foregoing Limited Warranty is in lieu of all other warranties, written or oral, express or implied, including, but not limited to, a warranty of merchantability, non-infringement, title or fitness for a particular purpose.

9 In no event shall seller be liable for any direct, indirect, consequential, punitive, incidental or any other damages of any kind whatsoever, arising out of or relating to the product, any product components, any specifications, documentation or other information provided to Customer in connection with the product, or the limited warrant set forth herein, even if Seller has been advised of, or otherwise should have been aware of, the possibility of such damages, and regardless of the legal theory or basis for such claim. - 8 - Introduction The Scope of this Manual Throughout the pages of this Manual , you will learn how to set up and operate your FlowCAM .

10 This Manual is intended to be used with both the Benchtop and Portable Series of FlowCAM models. Minor differences exist between the models and as such, will not affect the usefulness of this Manual . Additionally, this Manual is written to address and describe the configuration of a model VS-IV FlowCAM (AutoImaging, two Fluorescence Trigger channels, and one Scatter Trigger channel) equipped with a syringe pump. It must be recognized that some configurations of the FlowCAM system may not contain fluorescence, light scatter triggering/measurement capability, or a different type of pump. Please disregard any sections of this Manual which do not directly apply to your particular instrument.