Search results with tag "Miseq"
ランを成功させるための 最適なクラスター密度を得る方法
jp.illumina.comMiSeq MiSeq v3 1200 –1400 MiSeq v2 1000 –1200 NextSeq High/ Mid Output (v2.5 and v2) 170 - 220 ※ 上記推奨密度は塩基多様性に偏りのないライブラリーを使用した場合を想定
16Sサンプル調製ガイド15044223 A JPN - Illumina, Inc.
support.illumina.com3 MiSeqによるシーケンス―MiSeqv3試薬を使用した300bpのペアエンドリードにより、各 リードの終端が結合することで、V3-V4領域の高品質な全長リードが65時間のランで生成 されます。1回のMiSeqランではおよそ2,000万リード以上作成されるため、96サンプルの
NGSをはじめよう！これだけは知っておきたいMiSeq 解析原 …
jp.illumina.com3 MiSeqとは？ イルミナ社のデスクトップ型の 次世代シーケンサー（NGS） 1 ng~1 µgのDNA(RNA)サンプルから調製したライブラリー 相補鎖DNAを合成しながら読み取りを 行うSequencing By Synthesis (SBS法）を 用いる 最大15 Gbasesの塩基が解析可能！ 2013年6月時点
Nextera XT DNA Sample Preparation Kit - Illumina
www.illumina.comMiSeq Reporter provides automated on-instrument analysis for various applications including small genome de novo or resequencing, PCR amplicon, and plasmid sequencing. Sequencing results and analysis are easy to view and interpret. For example, using the PCR Amplicon workflow in the MiSeq Reporter software, sequence data are
Indexed Sequencing Overview for Illumina Systems
support-docs.illumina.comwith v1.0 reagent kits, MiniSeq with rapid reagent kits, MiSeq, HiSeq 2500, or HiSeq 2000 System is specific to the paired-end flow cell. Reading the i5 index requires seven additional chemistry-only cycles. This step uses the resynthesis mix, a paired-end reagent, during the Index 2 Read process.
イルミナシーケンサーの原理
www.clst.riken.jp– MiSeqケミストリーによる迅速サイクル – 150bp x2も可能に （※ Fast Run モードのみ適応） – 新フローセルを使用 *Anticipated performance at launch for new HiSeq 2500 systems. Official specifications will be set at launch. HiSeq 2500 システムパフォーマンス* 標準モード …
微生物16S/18S/ITS/功能基因 - CAS
www.ihb.cas.cn测序平台：Miseq（PE300） 主要结论：与对照组相比，投喂酵母显示出 在杆菌和梭状芽孢杆菌中的富集，而投喂 FPH（鱼蛋白水解物）显示出显著低于其他 两个喂养组的属于交替单胞菌和肠杆菌的细 菌数量。
Illumina Adapter Sequences - UC Davis
dnatech.genomecenter.ucdavis.eduHiSeq 2000/2500 and MiSeq i5 Bases for Sample Sheet NextSeq and HiSeq 3000/4000 . A505. GTAAGGAG CTCCTTAC. TruSight One. Index 1 (i7) Adapters . i7 Index Name i7 Bases for Sample Sheet. N701 . TAAGGCGA. N702 CGTACTAG N703 AGGCAGAA N704 TCCTGAGC
Lab SOP for Whole Genome Sequencing on Miseq
www.cdc.govprepping and pooling the libraries. 9.2.1. Pre-heat a heat block to 96°C ± 1. 9.2.2. Retrieve the pooled library plate/tubes, and if frozen, thaw on ice. Centrifuge at 800 – ... Dilute to 0.2 N by adding 400 µl of molecular grade water to the 100 µl aliquot of 1 …
Whole Genome Sequencing - NISC
www.nisc.nih.govOct 22, 2018 · A1. Whole genome sequencing (WGS) is simply the sequencing of the entire genome of an organism at one time [1]. The purpose may be to determine the genome sequence of a ... sequencing is performed on a MiSeq so read lengths are 300 bases, thus paired-end reads generate 600 bases of sequence from each fragment.
NGS超入門 - 愛媛大学
www.adres.ehime-u.ac.jpMiSeq は可逆的ターミネーター法として信頼のあるイルミナSequencing By Synthesis (SBS) 反応を用いてDNA クラスタをシーケンスする: Reversible Terminator Chemistry 1 反応に蛍光ラベルされた4 ヌクレオチドを加える (蛍光の種類で塩基を特定) 高精度 ホモポリマー領域も ...
NextSeq 500 System WGS Solution - Illumina, Inc.
www.illumina.comstudies on the Illumina MiSeq® System or large-scale WGS sequencing studies run on an Illumina HiSeq® System (Table 2). Simplified Bioinformatics in BaseSpace Illumina has removed much of the complexity from the typical informatics workflow. Bases generated on the NextSeq 500 System are instantly and securely
Optimizing Cluster Density on Illumina Sequencing Systems
www.illumina.com2500 in high output mode (Figure 4C), and the MiSeq® (Figure 4D) are shown. The blue or black tiles represent tiles that have intensities = 0 due to high cluster density. This is an indication that the flow cell is severely impacted. Figure 4: Flow Cell Charts.
The Genexus System - Thermo Fisher Scientific
assets.thermofisher.comMiSeq ™ System * Specimen-to-report workflow will be available after the Genexus Purification System and integrated reporting capabilities are added in 2020. ... • Plasma • Whole blood • Peripheral blood leukocytes (PBLs) • Lysate from fresh-frozen tissue • Lysate form bone marrow. 5 ... for complete genome sequencing and ...
Sequencing By Synthesis (SBS)
jp.illumina.comMiSeq. のフローセル（1枚につき1レーン） 変性した1本鎖 DNAライブラリー ... – Bentley et al. (2008) Accurate whole human genome sequencing using reversible terminator chemistry. Nature 456: 5359 -
MiSeq System - Illumina, Inc.
www.illumina.com1. Data calculations on file. Illumina, Inc., 2017. 2. Bentley DR, Balasubramanian S, Swerdlow HP, et al. Accurate Whole Human Genome Sequencing using Reversible Terminator Chemistry. Nature. 2008;456(7218):53-59. Table 1: MiSeq System performance parameters Read length Total timea Output quality scoresb Single reads cPaired-end reads MiSeq ...
MiSeq System Denature and Dilute Libraries Guide …
support.illumina.comMiSeq System Denature and Dilute Libraries Guide (15039740) Author: Illumina Subject: Instructions for denaturing and diluting libraries before sequencing on the MiSeq system. Created Date: 2/20/2019 5:43:03 PM
MiSeq System Guide (15027617) - Illumina, Inc.
support.illumina.comreagent cartridge, and then if required, denature and dilute libraries. Moved troubleshooting information to Appendix A. Moved output folder information and flow cell tile information to Appendix B. Moved information on managing files to the Maintenance chapter. Moved the post-run wash instructions from the Maintenance chapter to the Sequencing ...
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