glyphosaTe EPA METhoD 547 - Waters

1 [ ]11 glyphosaTe is a non-selective herbicide which is adsorbed through leaves and was first sold by Monsanto under the Roundup trade name. This is one of the most widely used herbicides, regularly used for agriculture, horticulture, and silviculture applications. The United States Environmental Protection Agency (US EPA) requires that drinking water and raw surface water be monitored for the presence of glyphosaTe and related compounds using EPA METhoD The European Union (EU) regulation (EC Directive 2005/70/EU) provides guidance with regards to the presence of glyphosaTe in drinking water ConditionsInstrument: Waters Alliance system for carbamate analysisEluent: phosphoric acid Column: Ion Exclusion, mm x 150 mm @ 55 CGuard: Guard-Pak module and insertsInjection: 200 L of standard mixFlow Rate: mL / minDetection: Fluorescence, Ex- 340 nm, Em- 455 nm, Gain- 10sample preparationRefer to EPA METhoD 547 for preparation of field grab samples.

2 Filter through m Acrodisc filters is sample preparationOasis MAX SPE METhoD for glyphosaTe and MetaboliteMethod for Oasis MAX Cartridge, 6 cc, 150 mg* Alternate eluent is 4 mL M sodium citrateUse 6 cc, 500 mg Oasis MAX for samples > 50 miX preparationPipette 100 L of AccuStandard mix (M-547) into 100 mL of acidified water for a concentration of 100. Prepare acidified water by adjusting the pH of HPLC grade water to by dropwise addi-tion of hydrochloric acid (HCL). Use EPA METhoD 547-02 as above for AMPA (aminomethyl phosphonic acid). eluent preparationDilute mL of 85% phosphoric acid (H3PO4) to 1 L, mix well, filter and PREPARE SAMPLE:pH 6-8 CONDITION:2 mL methanol, 4 mL M NaOH, 2 mL H2 ORINSE:4 mL M NaOHRINSE:2 mL H2 OLOAD:25 mL sampleWASH:2 mL H2 OELUTE:4 mL M HCI in acetonitrile*EVAPORATE AND RECONSTITUTE:Adjust to LC analysisMeasurement of C hloracetanilide and other Acetamide herbicide Degradates in Drinking Water by Solid Phase Extraction and Liquid Chromatography/Tandem Mass Spectrometry (LC/MS/MS)D e t e r m i n a t i o n o f G l y p h o s a t e i n D r i n k i n g W a t e r b y D i r e c t A q u e o u s I n j e c t i o n h P L C , Post Column Derivatization and Fluorescence DetectionEPA METhoD 5 47.

3 [ ]12post Column reagent preparationReagent 1: hypochlorite Dissolve g KH2PO4, g NaCl, g NaOH, and mL Clorox Bleach (plain) in water and dilute to 1 L, filter and 2: oPA Dissolve g of OPA (o-phthdialdehyde) in 10 mL of methanol, add this to an aqueous solution of g of Borax Na2B4O7 10H2O. Make to a final volume of 1 L, filter and degas. To this, add 2 mL of 2-mercaptoethanol, swirl gently to mix. Protect from : Post-column flow rate for both reagents is mL/min, post column reaction tempera-ture is 38 C. Insert second reaction coil in line before the fluorescence detector. Standard chromatogram, 100 ppb each information Related PartsPart NumberIC-Pak Ion-Exclusion Column, x 150 mmWAT 010295 Guard-Pak HolderWAT 8 8141 Semivolatiles #2 Herbicide Standard186004271 Oasis MAX Cartridge, 6 cc, 150 mg186000370 Oasis MAX Cartridge, 6cc, 500 mg186000865 Related DocumentsLiterature CodeEnvironmental System Solutions720001601 ENGlyphosate and AMPA in Drinking LC/MS/MS Multi-Analyte Detection METhoD for Deleterious Organics in Drinking Water720001090 E EUPeakAnalyte1 Glyphosate2AM PAdiquaT and paraquaTDetermination of glyphosaTe in Drinking Water by Direct Aqueous Injection hPLC, Post Column Derivatization and Fluorescence DetectionEPA METhoD 5 47.

4 0 2008 Waters Corporation. Waters , T he Science of W hat s Possible, Alliance, Guard-Pak, IC-Pak and Oasis are trademarks of Waters Corporation. AccuStandard is a registered trademark of AccuStandard Inc. Acrodisc is a registered trademark of Pall Corporation. Monsanto and Roundup are registered trademarks of T he Monsanto Company.