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GOOD LABORATORY PRACTICE for HPLC
spent troubleshooting spurious peaks, baseline noise, etc… I-1. Quality All reagents and solvents should be of the highest quality. HPLC grade reagents may cost slightly more than lower grade reagents, but the difference in purity is marked. HPLC grade reagents contain no impurities to produce spurious
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Molecular Techniques and Methods Native Gel Electrophoresis
www.ispybio.com18. Remove the dye that is not bound to protein in Destaining Solution. After about 24 hours, with gentle agitation and several changes of Destaining Solution, the gel background becomes colorless and leaves protein bands colored blue, purple, or red. Coomassie Brilliant Blue R250 and PAGE Blue 83 each visibly stain as little as 0.1-1 ug of protein