HPLC CoLumn umn CHeCK stAnDARDs - Phenomenex

1 HPLC CoLumn CHeCK stAnDARDs . We recommend using CHeCK stAnDARDs to verify performance of all new columns and periodically over their lifetime. stAnDARDs for all Phenomenex brand name columns are available. stAnDARDs are grouped by CoLumn type ( normal phase standard for Si, -NH2, -Diol, -NO2, alumina and PAC). Normal Phase Carbohydrate Mix 1 Organic Acid Standard App ID 14743. App ID 14745. App ID 14749. Part No.: ALO-3033 Price: Part No.: ALO-3035 Price: Part No.: ALO-3039 Price: (For Si, NH2, NO2, Diol, Alumina, PAC and Luna CN) (For Rezex RNM, RAM & other carbohydrate analysis (For Rezex ROA and other organic acid analysis). Unit quantity: 2 mL columns) Unit quantity: 2 mL. Contains: Meta-xylene; Nitrobenzene Unit quantity: 2 mL Contains: Oxalic acid; Citric acid; Tartaric acid; HPLC. Diluent: Hexane/Acetonitrile (99:1) Contains: Maltotriose Hydrate; Maltose; Ribitol Succinic acid; Formic acid; Acetic Test Conditions Diluent: Water acid Mobile phase: Hexane/Acetonitrile (99:1) Test Conditions Diluent: 5 mM Sulfuric Acid Flow rate: mL/min Mobile phase: Water Test Conditions (for a 300 x mm CoLumn ).

2 Injection volume: L Flow rate: mL/min for a 300 x mm CoLumn Mobile phase: H2SO4. Detection: UV @ 254 nm Temperature: 85 C Flow rate: mL/min HPLC CoLumn CHeCK stAnDARDs Injection volume: L Temperature: ambient Detection: RI Injection volume: L. Detection: UV @ 210 nm Reversed Phase 1. App ID 14744. Part No.: ALO-3034 Price: Carbohydrate Mix 2. App ID 14746. Cation-Exchange App ID 14750. (For C1, C18, CN and Phenyl). Unit quantity: 2 mL Part No.: ALO-3036 Price: (For Rezex RPM & other carbohydrate analysis columns) Part No.: ALO-3040 Price: Contains: Uracil; Benzamide; Benzene; Benzophenone; Biphenyl (refer to Unit quantity: 2 mL (For SCX, SA, CM). product insert for specific details) Contains: Melezitose; Glucose; Fructose; Ribitol Unit quantity: 2 mL. Diluent: Acetonitrile Diluent: Water Contains: Uracil; Cytosine Test Conditions Diluent: Water Test Conditions Mobile Phase: Acetonitrile/Water (percentages Mobile phase: Water Test Conditions depend on phase) Flow rate: mL/min for a 300 x mm CoLumn Mobile phase: M (NH4)2 HPO4 , pH Flow rate: mL/min Temperature: 85 C Flow rate: mL/min Injection volume: L for 3 and 5 m particles* Injection volume: L Injection volume: L.

3 L for 10 m particles Detection: RI Detection: UV @ 254 nm Detection: UV @ 254 nm *For Onyx C8, C18; Ultracarb C8, ODS(20), ODS(30) inject L for all CoLumn sizes. Carbohydrate Mix 3 Anion-Exchange App ID 14747. App ID 14751. Part No.: ALO-3037 Price: Part No.: ALO-3041 Price: Reversed Phase 2 (For Rezex RCM, RCU & other carbohydrate analysis columns) (For SAX, SB, DEAE, PEI). App ID 14754. Unit quantity: 2 mL Unit quantity: 2 mL. Part No.: ALO-3045 Price: Contains: Melezitose; Mannose; Maltose; Contains: Uridine, UMP (refer to product insert Fructose; Glucose; Ribitol for specific details). (For Prodigy C8, ODS(2), ODS(3); Luna C5, C8, C18, Diluent: Water Diluent: Water Phenyl-Hexyl; Jupiter C4, C5, C18; Jupiter Proteo;. Columbus C8, C18; Aqua; PhenoSphereNEXT C8, C18; Test Conditions Test Conditions Synergi; Gemini C18, C6-Phenyl) Mobile phase: Water Mobile phase: M (NH4)2 HPO4 , pH Unit quantity: 2 mL Flow rate: mL/min for a 300 x mm CoLumn Flow rate: mL/min Contains: Uracil; Acetophenone; Benzene; Temperature: 85 C Injection volume: L.

4 Toluene; Naphthalene Injection volume: L Detection: UV @ 254 nm Diluent: Acetonitrile Detection: RI. Test Conditions for Jupiter C18, all Luna (except CN), Aqua, Synergi; Gemini, Prodigy, and Columbus columns Mobile phase: Acetonitrile/Water (65:35)* Oligosaccharide Standard App ID 14748. Flow rates and Injection volumes Flow rate: mL/min; mL/min for 3 m particles are for 250 x mm size columns, Part No.: ALO-3038 Price: Injection volume: L unless otherwise noted. Detection: UV @ 254 nm (For Rezex RSO, RNO & other oligosaccharide analysis columns). Unit quantity: 2 mL. Test Conditions for Jupiter C4 and C5 columns Contains: Light corn syrup Mobile phase: Acetonitrile/Water (50:50) Diluent: Water Flow rate: mL/min Injection volume: L Test Conditions Detection: UV @ 254 nm Mobile phase: Water Flow rate: mL/min for a 200 x 10 mm CoLumn *Columns with dimensions of 50 x mm, 30 x or Temperature: 85 C. mm, the mobile phase ratio should be 50:50. Some 50 Injection volume: L. x mm columns use 65:35.

5 Detection: RI. Phenomenex 87. HPLC CoLumn CHeCK stAnDARDs . Aqueous Sec 1 Chiral Test Mix Chiral Test Mix App ID 14752. App ID 14755. App ID 14757. Part No.: ALO-3042 Price: Part No.: ALO-3046 Price: Part No.: ALO-3048 Price: (For BioSep SEC-S and other protein SEC columns) Applicable to the following Chirex columns: Applicable to the following Chirex columns: Unit quantity: Dry; Reconstituted to 2 mL Chirex Phase Bond Chirex Phase Bond Contains: Bovine thyroglobulin; Human Phase Description Type Phase Description Type gamma globulin; Ovalbumin; (R)-Phenylglycine-DNB Covalent 3001 3014 (S)-Valine and (R)-1- -NEA Covalent Myoglobin; Uridine (reconstitute 3005 (R)-Naphthylglycine and DNB Covalent 3017 (S)-Proline and (S)-1- -NEA Covalent with 1 mL of 100 mM Phosphate 3018 (S)-Proline and (R)-1- -NEA Covalent Unit quantity: 2 mL buffer pH= ) 3019 (S)-tert-Leucine and (S)-1- -NEA Covalent Contains: (+/-)-2,2,2-trifluoro-1-(9-anthryl) Diluent: 100 mM Sodium phosphate buffer, 3020 (S)-tert-Leucine and (R)-1- -NEA Covalent ethanol CAS [60646-30-2] and pH 3022 (S)-Indoline carboxylic acid and Covalent [53531-34-3].

6 Storage: Add % NaN3 to the solution and (R)-1- -NEA. Diluent: Hexane/Ethanol refrigerate (NEA = Naphthylethylamine). (95:5). Test Conditions Unit quantity: 2 mL. Test Conditions Mobile phase: 100 mM Sodium phosphate buffer, Contains: (+/-)-N-(3,5-Dinitrobenzoyl)- - Mobile Phase: Hexane/Ethanol pH HPLC. (95:5) methylbenzylamine CAS [69632-32- Flow rate: mL/min for a 300 x mm CoLumn 2] and [69632-31-1]. Flow Rate: mL/min Injection volume: 10 L Diluent: Hexane/Isopropanol/Ethanol Injection Volume: L. Detection: UV @ 280 nm (70:20:10). Detection: UV @ 254 nm Test Conditions Mobile Phase: Hexane/Isopropanol/Ethanol HPLC CoLumn CHeCK stAnDARDs (70:20:10). Aqueous Sec 2 Chiral Test Mix Flow Rate: mL/min App ID 14753. App ID 14756. Injection Volume: L. Part No.: ALO-3043 Price: Part No.: ALO-3047 Price: Detection: UV @ 254 nm (For PolySep GFC-P and other aqueous-soluble analysis Applicable to the following Chirex columns: columns) Chirex Phase Bond Unit quantity: 2 mL Phase Description Type Chiral Test Mix App ID 14758.

7 Contains: Ethylene Glycol 3010 (S)-Valine and DNAn Covalent Diluent: Water 3011 (S)-Leucine and DNAn Covalent Part No.: ALO-3049 Price: Test Conditions 3012 (R)-Phenylglycine and DNAn Covalent Applicable to the following Chirex columns: Mobile Phase: Water (DNAn = 3,5-Dinitroaniline). Flow rate: mL/min Chirex Phase Bond Unit quantity: 2 mL Injection volume: L Phase Description Type Contains: N-dansyl-DL-valine Detection: RI (cyclohexylammonium salt); 3126 N,S-dioctyl-(D)-Penicillamine Ionic CAS[84540-67-0] Unit quantity: 2 mL. Diluent: 10 mM ammonium acetate in Contains: DL-Aspartic Acid CAS [617-45-8]. methanol Diluent: 2 mM Copper sulfate pentahydrate in STAR-ION A300. App ID 14759. Test Conditions water/Isopropanol (95:5). Mobile Phase: 10 mM ammonium acetate in Test Conditions Part No.: ALO-3420 Price: methanol Mobile Phase: 2 mM Copper sulfate pentahydrate in Unit quantity: 2 mL Flow Rate: mL/min water/Isopropanol (95:5). Contains: Fluoride (5 mg/mL); Chloride Injection Volume: L Flow Rate: mL/min (10 mg/mL); Nitrite (20 mg/mL); Detection: UV @ 254 nm Injection Volume: L.

8 Bromide (20 mg/mL); Nitrate Detection: UV @ 254 nm (20 mg/mL); Phosphate (30 mg/mL);. Sulfate (20 mg/mL). Diluent: mM NaHCO3 mM Na2CO3 ONYX Monolithic Normal Phase App ID 15639. Test Conditions ONYX Monolithic Reversed Phase App ID 15640. Mobile phase: mM NaHCO3 mM Na2CO3 Part No.: ALO-7835 Price: Flow rate: mL/min for a 100 x mm For Si (Silica) Part No.: ALO-7836 Price: CoLumn Unit quantity: 2 mL For C8 and C18. Injection volume: 20 L Contains: Toluene g/mL; Unit quantity: 2 mL. Detection: Suppressed Conductivity Nitrobenzene 150 g/mL; Contains: Thiourea 10 g/mL; 2-Nitroanisol g/mL Progesterone 100 g/mL; Diluent: n-heptane/Dioxane (95:5) Anthracene 10 g/mL Test Conditions Diluent: Acetonitrile/Water (60:40). PolymerX RP-1. App ID 14760. Mobile phase: n-heptane/Dioxane (95:5) Test Conditions Flow rate: mL/min* Mobile phase: Acetonitrile/Water (60:40). Part No.: ALO-7260 Price: Injection volume: L Flow rate: mL/min*. Unit quantity: 2 mL Detection: UV @ 254 nm Injection volume: L.

9 Contains: Cytosine 13 mg/mL; Storage Conditions: Refrigerate @ 4 C Detection: UV @ 254 nm Uracil 13 mg/mL; *for a 100 x mm CoLumn Storage Conditions: Refrigerate @ 4 C. Uridine 33 mg/mL *for a 50 x mm CoLumn Diluent: Water Test Conditions Mobile phase: M Citric Acid, See p. 45 for GC CoLumn Flow rate: mL/min*. Temperature: Ambient Performance CHeCK stAnDARDs . Injection volume: 5 L. Detection: UV @ 254 nm See p. 343 for comprehensive line Storage Conditions: Refrigerate @ 4 C of Polymer Calibration stAnDARDs . *for a 5 m 250 x mm CoLumn Flow rates and Injection volumes are for 250 x mm size columns, unless otherwise noted. 88 Phenomenex