Kinetex - Phenomenex

1 186 PhenomenexKINETEX | HPLCU ltra-High Performance on Any LC System Achieve sub-2 m performance within HPLC backpressure limitations Substitute 3 m and 5 m columns for 2-3x higher efficiency Obtain higher throughput without sacrificing resolution Easy method transfer across LC system platforms Reduce solvent consumption with faster analysis Reach lower levels of detection and quantitation Material Characteristics*Packing MaterialTotal Particle Size ( m)Porous Shell ( m)Solid Core ( m)PoreSize( )Effective Surface Area (m2/g)Effective Carbon Load %pH StabilityPressure StabilityKinetex - 10**1000/600 barKinetex - 10** Kinetex - - - - 10**1000 barKinetex - 10** Kinetex - - - *For the evaluation of effective surface area and carbon load, please request technical note, TN-1064** columns are pH stable from under isocratic conditions.

2 columns are pH stable 2-8 under gradient mm ID Kinetex columns are pressure stable up to 1000 m Core-Shell ParticleReduced diffusion path maximizes efficiencyIncreased efficiencies compared to traditional fully porous sub-2 m columns . Typical operating backpressures > 400 bar When using Kinetex m, increased performance can be achieved, however higher pressure-capable instrumentation is in Particle Technology The Kinetex core-shell particle is not fully porous. Using sol-gel processing techniques that incorpo-rate nano structuring technology, a durable, homogenous porous shell is grown on a solid silica core. This highly optimized process combined with uniform particle size distribution produces a column that generates extremely high plate counts.

3 When using Kinetex m, less column backpressure is generated, allowing it to be used on any LC system. Kinetex m Core-Shell ParticleReduced diffusion path maximizes efficiencyUltra-high performance on any system with Kinetex m m Porous m Solid m Porous m Solid Core SEM of Kinetex Core-Shell ParticlesnewCross Section of Kinetex Core-Shell Particle2010 R&D 100 Award RecipientIf you are not completely satisfied with Kinetex core-shell columns , send in your comparative data to a similar product within 45 days and KEEP THE COLUMN FOR 187 PhenomenexHPLC | KINETEXSub-2 m Fully PorousBand BroadeningSince the Kinetex particle is not fully porous.

4 Analytes spend less time diffusing into and out of the pores as they travel through the column. This shorter diffusion path allows for faster mass transfer. The result is less band broadening for higher peak efficiency comparable to or better than sub-2 m fully porous Velocity (mm/s)HETP ( m)6789103 mDouble the efficiency of 3 m at high speedSub-2 m exceeds conventional pressure here Kinetex m Core-Shell Sub-2 m Fully Porous 3 m Fully m Kinetex Core-ShellBand Broadening Optimized for Ultra-High PerformanceFaster Mass TransferHigh Efficiency over Extended Range of Flow Rates3 m Fully PorousBand BroadeningKinetex particles are nearly monodispersed.

5 This extremely narrow particle size distribution results in ultra-high column efficiency and excellent Count10000123 Particle Diameter ( m)456 Kinetex m Core-Shell Traditional Fully Porous m Traditional Fully Porous 3 m Uniform Particle Size DistributionBatch-to-Batch Reproducibility OverlayBatch 68 Rs (2,3) 73 Rs (2,3) 75 Rs (2,3) 68 Batch 73 Batch 75 App ID 18755, 18756, 18757 Conditions same for all batches: Column: Kinetex m C18 Dimensions:50 x mmPart No.:00B-4462-E0 Mobile Phase: Water / Acetonitrile (65:35)Flow Rate: mL/minDetection:UV @ 254 nmSample: 1. Uracil2. Hydroxycortisone3. Cortisone4.

6 Cortisone acetate5. 17-HydroxyprogesteroneKinetex 188 PhenomenexKINETEX | HPLC012 min181650200400mV12340123412 min0200400mV18166 With the efficiency of a sub-2 m column and typical operating backpressure less than 400 bar , you can achieve the promise of ultra-high performance on any LC system. N = 267,720 p/m< 300 bar! Easier method transfer to ANY system Kinetex m columns , mm ID, are pressure rated to 1000 bar use on both HPLC and UHPLC instrumentation.* Waters, ACQUITY, and UPLC are registered trademarks of Waters Corporation. Phenomenex is not affiliated with Waters Corporation. Comparative separations may not be representative of all min181650200400mV1234 Achieve Sub-2 m Performance within HPLC Backpressure LimitationsUnparalleled Levels of Ultra-High PerformanceN = 272,080 p/m> 400 bar!

7 Method cannot transfer to a non-UHPLC instrumentKinetex m Traditional mKinetex mTraditional mFor users of higher pressure capable instruments who want the highest level of efficiency, we introduce the Kinetex m column - the first sub-2 m core-shell particle available on the = 272,080 p/mApp ID 18165181671234012 min0200400mVN = 318,680 p/mApp ID 18167 App ID 18165 App ID 18166 Conditions for both columns :Column: Kinetex m C18 Traditional m C18 Dimensions:50 x mmMobile Phase: Acetonitrile / Water (50:50) Flow Rate: mL/minTemperature: 25 CDetection:UV @ 254 nmInstrument: *Waters ACQUITy UPLC Sample: L test mixture 1.

8 Acetophenone 2. Benzene 3. Toluene 4. NaphthaleneConditions for both columns :Column: Kinetex m C18 Traditional m C18 Dimensions:50 x mmMobile Phase: Acetonitrile / Water (50:50) Flow Rate: mL/minTemperature: 25 CDetection:UV @ 254 nmInstrument: *Waters ACQUITy UPLC Sample: 1. Acetophenone 2. Benzene 3. Toluene 4. NaphthaleneKinetex 189 PhenomenexHPLC | KINETEX18280012345678910111213204060 minKinetex mReplace traditional 3 m or 5 m analytical columns with Kinetex m core-shell columns for immediate performance improvements in efficiency, speed, resolution, and sensitivity. Optimize methods for ultra-high performance and transfer them to any separations may not be representative of all for both columns :Column: Kinetex m C18 Traditional 3 m C18 Dimensions:150 x mmMobile Phase: Acetonitrile / Water (70:30)Flow Rate: mL/minTemperature: 25 CBackpressure:380 bar ( Kinetex )250 bar (Traditional 3 m)Detection:UV @ 254 nmInstrument: Agilent 1200 SLSample: 1.

9 Uracil 2. Acetophenone 3. Toluene 4. NaphthaleneN = 295,343 p/m186280224431min0306090120150180210240 mV030609012015018021024002mV4min18539123 4 App ID 18628 For the ultimate sample throughput demands, Kinetex columns provide the efficiency needed to significantly reduce run times. In this separation of 13 ketones, a 20-fold increase in productivity is accomplished while still maintaining :Traditional 5 m C18 Dimensions:250 x mmMobile Phase: A: WaterB: Acetonitrile Gradient:Time (min) % B 0 5 5 95 Time (min) % B 66 95 5 5 Flow Rate: mL/minTemperature: 45 CDetection:UV @ 258 nmSample: 1. Acetone 2. 2-Butanone 3.

10 2-Pentanone 4. Acetophenone 5. 2-Heptanone 6. Butyrophenone 7. 2-Nonanone 8. Hexanophenone 9. Octanophenone 10. 2-Tridecanone 11. Decanophenone 12. 2-Pentadecanone 13. 2-HexadecanoneColumn: Kinetex m C18 Dimensions:50 x mmPart No.:00B-4462-E0 Mobile Phase: A: WaterB: AcetonitrileGradient:Time (min) % B 0 5 5 95 Time (min) % B 95 5 5 Flow Rate: mL/minTemperature: 45 CDetection:UV @ 258 nmBackpressure:350 barSample: 1. Acetone 2. 2-Butanone 3. 2-Pentanone 4. Acetophenone 5. 2-Heptanone 6. Butyrophenone 7. 2-Nonanone 8. Hexanophenone 9. Octanophenone 10. 2-Tridecanone 11.