Introduction - Microchem

1 Introduction : Microchem Lab Services (Pty) Ltd is an independent and authoritative provider of a comprehensive range of chemical and microbiological analyses and related services to the food and non-food industries. Our aim is to offer comprehensive chemical, nutritional, environmental, microbiological, and food borne pathogen analysis to clients. Microchem was established by Raymond Hartley in 2007 when he bought the Woolworths in-house laboratory. In 2009 Microchem acquired Consulting Microbiological Laboratory (CML) in Gauteng. CML was a well known microbiological laboratory, the acquisition allows for greater opportunities to service Microchem s Gauteng clients who require microbiological services. Our Services: Routine food microbiology analyses Food borne pathogen analyses Water microbiological analyses Product shelf-life analyses Foreign object inspections Hygiene inspections and reports Microbiological swabbing Food chemistry Nutritional analyses Trace elemental and heavy metal analyses Food colorants (Azo Dyes) Vitamin analyses Water chemical analyses Melamine analyses Pesticide analyses Consulting services: Our specialists provide consultation on the assessments and advice on environmental hygiene management programmes, interpretation of analytical results and advice on nutritional analyses, as well as assistance with pathogen management in production facilities.

2 Quality: Microchem is a South African National Accreditation System (SANAS) accredited laboratory that uses a quality system which meets national and international criteria (EN45001, ISO 17025). The analytical methods employed are based on nationally and internationally accepted procedures. Facilities: To meet your analytical requirements our modern facilities include centrally situated laboratories with over 1000 square meters of laboratory space for adequate sample reception, analyses and storage. Microchem continuously invests in the latest technology, Our state of the art technology includes GC, GC/MS, GS-MS/MS, ICP-OES, HPLC, LC-MS/MS, real time PCR, Tempo, Vidas and LIMS. These instruments have greatly broadened the range of analyses, and enable us to test for substantially lower levels of detection, and allows for the analysis of whole new classes of compounds. Personnel: Our laboratories are staffed with advanced degree chemist, microbiologists, biotechnologists, food technologists, and highly trained analysts to provide an exemplary and personalized service.

3 The personnel s strong, multi-skilled technical backgrounds and experience in a variety of analytical specialties ensure that our laboratory stays in the forefront of technology to provide superior results. Why use Microchem ? We meet the needs of our clients by providing: Quality assured results (SANAS accredited) Excellent service The convenience of one-stop lab solution Rapid turnaround times Competitive prices Contact Information: Cape Town (Head Office) 1st Floor Fairweather House, 176 Sir Lowry Road Woodstock, Cape Town, South Africa, 8001 Tel: +27 (21) 465-6996 Managing Director: Raymond Hartley Gauteng Micro Laboratory 41 Golden Drive, Morehill, Benoni, South Africa, 1501 Tel: +27 (11) 425-3775 Please visit our website for additional information: Tests: ANALYSES SANAS Accredited Lead times (Days) Method used Instrument LOD Sample amount required Nutritional Analyses 10 to 14 min 350g % Moisture Yes AOAC Drying in air oven % Ash Yes AOAC Ash by Furnace % Protein (% Nitrogen included in protein analysis) Yes AOAC Leco Protein analyser % Total fat and Fatty Acid Profile (Including the following): Yes AOAC GC % Saturated Fat Yes AOAC GC - % Mono unsaturated fat Yes AOAC GC - % Poly unsaturated fat Yes AOAC GC - % Trans fat Yes AOAC GC - % Omega 3 (EPH/DHA), 6 & 9 Yes AOAC GC - mg Cholesterol Yes AOAC GC % Glycaemic carbohydrates Yes - % Total sugars.

4 (Used for Glycaemic Carbohydrates) Yes AOAC (amended) GC - % Fructose Yes AOAC (amended) GC % Glucose Yes AOAC (amended) GC % Sucrose Yes AOAC (amended) GC % Maltose Yes AOAC (amended) GC % Galactose Yes AOAC (amended) GC % Trehalose Yes AOAC (amended) GC % Lactose Yes AOAC (amended) GC % Starch (Used for Glycaemic Carbohydrates) Yes AOAC HPLC % Total dietary Fibre Yes AOAC Gravimetric - mg Sodium Yes AOAC (amended) ICP-OES KJ/100g Energy (by calculation) Yes Calculated % Carbohydrates (Calculated) Yes Calculated Chemical Analyses % Total dietary Fibre (Part of full nutritional analyses) Yes 10 to 14 AOAC Gravimetric - & Total dietary fibre (Including fat,moisture and sugar) Yes 10 to 14 AOAC Gravimetric - % Soluble and Insoluble dietary fibre No 10 to 14 AOAC Gravimetric - pH No 2 pH meter - Brix No 2 Handheld refractometer - % Acidity (total acidity) Yes 5 to 10 AOAC Titration - % Salt (as NaCL) Yes 5 to 10 Metrohm application Autotitrator NaCL Peroxide value (milli equivalence) on fat or oil No 5 to 10 AOAC Titration - Peroxide value (food products containing > 20% fat No 5 to 10 AOAC Titration - % Free fatty acids- (By titration only on fats or oils) No 5 to 10 AOAC Titration - & Free fatty acids (Food products containing > 20% fat))

5 No 5 to 10 AOAC Titration - ppm Sulphur dioxide No 14-20 AOAC - Vitamins Analyses min 100g Vitamin A- Retinol Yes 5 to 10 AACC 86-06 HPLC Vitamin E- Tocopherol Yes 5 to 10 AACC 86-06 HPLC Vitamin C- Ascorbic acid Yes 5 to 10 AOAC HPLC Vitamin B1- Thiamine Yes 5 to 10 AOAC HPLC Vitamin B2- Riboflavin Yes 5 to 10 AOAC HPLC Vitamin B3- Niacin Yes 5 to 10 AOAC HPLC Vitamin B5- Pantothenic acid No 5 to 10 - LC-MS - Vitamin B6- Pyridoxine Yes 5 to 10 AOAC HPLC Vitamin B7- Biotin No - LC-MS - Vitamin B9- Folic acid No - LC-MS - Elemental Analyses min 100g Aluminium (ICP-OES analysis) No 5 to 10 AOAC (amended) ICP-OES - Arsenic (ICP-OES analysis) Yes 5 to 10 AOAC (amended) ICP-OES Barium (ICP-OES analysis) No 5 to 10 AOAC (amended) ICP-OES - Bismuth (ICP-OES analysis) No 5 to 10 AOAC (amended) ICP-OES - Boron (ICP-OES analysis) No 5 to 10 AOAC (amended) ICP-OES - Cadmium (ICP-OES analysis) Yes 5 to 10 AOAC (amended) ICP-OES Calcium (ICP-OES analysis) Yes 5 to 10 AOAC (amended) ICP-OES Chromium (ICP-OES analysis) No 5 to 10 AOAC (amended) ICP-OES - Cobalt (ICP-OES analysis) No 5 to 10 AOAC (amended) ICP-OES - Copper (ICP-OES analysis) Yes 5 to 10 AOAC (amended) ICP-OES Gallium (ICP-OES analysis) No 5 to 10 AOAC (amended) ICP-OES - Indium (ICP-OES analysis) No 5 to 10 AOAC (amended) ICP-OES - Iron (ICP-OES analysis) Yes 5 to 10 AOAC (amended) ICP-OES Lead (ICP-OES analysis) Yes 5 to 10 AOAC (amended) ICP-OES Lithium (ICP-OES analysis) No 5 to 10 AOAC (amended) ICP-OES - Magnesium (ICP-OES analysis) Yes 5 to 10 AOAC (amended) ICP-OES Manganese (ICP-OES analysis) Yes 5 to 10 AOAC (amended) ICP-OES Mercury (ICP-OES analysis)

6 Yes 5 to 10 AOAC (amended) ICP-OES Molybdenum (ICP-OES analysis) No 5 to 10 AOAC (amended) ICP-OES - Nickel (ICP-OES analysis) No 5 to 10 AOAC (amended) ICP-OES - Phosphorus (ICP-OES analysis) No 5 to 10 AOAC (amended) ICP-OES Potassium (ICP-OES analysis) Yes 5 to 10 AOAC (amended) ICP-OES Selenium (ICP-OES analysis) No 5 to 10 AOAC (amended) ICP-OES - Silver (ICP-OES analysis) No 5 to 10 AOAC (amended) ICP-OES - Sodium (ICP-OES analysis) Yes 5 to 10 AOAC (amended) ICP-OES Strontium (ICP-OES analysis) No 5 to 10 AOAC (amended) ICP-OES - Sulphur (ICP-OES analysis) No 5 to 10 AOAC (amended) ICP-OES - Thallium (ICP-OES analysis) No 5 to 10 AOAC (amended) ICP-OES - Tin (ICP-OES analysis) No 5 to 10 AOAC (amended) ICP-OES - Zinc (ICP-OES analysis) Yes 5 to 10 AOAC (amended) ICP-OES Water Analyses - SANS 241 10 to 14 min 1L of water Aluminium No 5 to 10 AOAC (amended) ICP-OES - Ammonia as N No 5 to 10 Spectrophotometer - Arsenic as As Yes 5 to 10 AOAC (amended) ICP-OES Cadmium as Cd Yes 5 to 10 AOAC (amended) ICP-OES Cloride as Cl No 5 to 10 Spectrophotometer - Cobalt as Co No 5 to 10 AOAC (amended) ICP-OES - Conductivity No 5 to 10 Conductivity meter - Copper as Cu Yes 5 to 10 AOAC (amended) ICP-OES Cyanide (recoverable) as CN No 5 to 10 Spectrophotometer - Flouride as F No 5 to 10 Spectrophotometer - Iron as Fe Yes 5 to 10 AOAC (amended) ICP-OES Lead as Pb Yes 5 to 10 AOAC (amended) ICP-OES Managanese as Mn Yes 5 to 10 AOAC (amended) ICP-OES Mercury as Hg Yes 5 to 10 AOAC (amended) ICP-OES Nickel as Ni No 5 to 10 AOAC (amended)

7 ICP-OES - Nitrate as N No 5 to 10 Spectrophotometer - Nitrite as N No 5 to 10 Spectrophotometer - pH No 5 to 10 pH meter - Selenium as Se No 5 to 10 AOAC (amended) ICP-OES - Sodium as Na Yes 5 to 10 AOAC (amended) ICP-OES Total chromium as Cr No 5 to 10 AOAC (amended) ICP-OES - Total dissolved solids No 5 to 10 Conductivity meter - Vanadium as V No 5 to 10 AOAC (amended) ICP-OES - Zinc as Zn Yes 5 to 10 AOAC (amended) ICP-OES Strontium No 5 to 10 AOAC ICP-OES - Thallium No 5 to 10 AOAC ICP-OES - Other Chemical Analyses Azo dyes: which includes Yes 10-15 In house developed LC-MS - min 100g Sudan 1 Yes 10-15 LC-MS - Sudan 2 Yes 10-15 LC-MS - Sudan 3 Yes 10-15 LC-MS - Sudan 4 Yes 10-15 LC-MS - Para Red Yes 10-15 LC-MS - Methyl Yellow Yes 10-15 LC-MS - * Additional cost if Erythorosine present in sample No Melamine screening Yes 10-15 In house developed LC-MS - min 100g Melamine quantification No 10-15 LC-MS - Sunscreen Analysis No 10-15 In house developed Zinc oxide No 10-15 CAPE TOWN MICROBIOLOGICAL ANALYSIS SANAS Accredited Lead times Days Method used Instrument LOD Sample Amount Routine Micro min 100g TVC (Total Viable Count) Yes 3 MFHPB-18 <10 CFUs/g Enterobacteriaceae Yes 2 ISO 7402 <10 CFUs/g Coliforms Yes 2 ISO 4832/ISO 5541/1 <10 CFUs/g Yeast and Mould Yes 6 Oxoid-Acidified PDA <10 CFUs/g Lactic acid bacteria Yes 4 ISO 15214.

8 1998 <10 CFUs/g Osmophillic yeasts and moulds Yes 6 Oxoid-Modified Wort agar <10 CFUs/g Pseudomonas spp. Yes 3 CCFRA Method : 1995 <10 CFUs/g Commercial sterility No 6 MFHPB-01 <10 CFUs/g Species ID: Gram negative bacteria No 3 Oxoid n/a Sporeformers: Aerobic No 3 MFLP- 44 <10 CFUs/g Sporeformers: Anaerobic No 3 MFLP- 44 <10 CFUs/g Pathogens 25g/pathogen E. coli (confirmed) Yes 2 Oxoid-TBX agar <10 CFUs/g Staphylococcus aureus (confirmed) Yes 2 ISO 6888-2 <10 CFUs/g Listeria monocytogens (detection) Yes 3 AFNOR BRD: 07/04 -09/98 <10 CFUs/25g Listeria monocytogens (confirmation) Yes 2 AFNOR BRD: 07/04 -09/98 n/a Listeria monocytogens (biochemical confirmation) Yes 3 AFNOR BRD: 07/04 -09/98 n/a Listeria monocytogens count Yes 2 AFNOR BRD: 07/05 -09/01 <10 CFUs/25g E. coli 0157 (detection) No 3 Oxoid-SMAC agar with BCIG <10 CFUs/25g E. coli 0157 (confirmation) No 2 Oxoid n/a Salmonella (detection) Yes 4 AFNOR BRD 07/11 12/05 <10 CFUs/25g Salmonella (confirmation) Yes 3 AFNOR BRD 07/11 12/05 n/a Bacillus cereus (presumptive count) No 2 Oxoid-Brilliance <10 CFUs/g Clostridium perfringens (detection) No 2 Oxoid-OPSP Agar <10 CFUs/g Clostridium perfringens (confirmation) No 2 Oxoid <10 CFUs/g Vibrio spp (confirmed) No 7 Lab developed <10 CFUs/25g Water analyses TVC (water) Yes 4 Oxoid-R2A <1 CFUs/ml 50ml E.

9 Coli (water) Yes 2 AFNOR BRD 07/7-12/04 <1 CFU/ volume filtered 250ml Coliforms (water) Yes 2 AFNOR BRD 07/8 -12/04 <1 CFU/ volume filtered 250ml Pseudomonas spp (water) Yes 3 CCFRA Method : 1995 <1 CFU/ volume filtered 250ml Enviromental Swabs TVC Yes 3 MFHPB-18 <10 CFUs/area swabbed 1 swab Enterobacteriaceae Yes 2 ISO 7402 <10 CFUs/area swabbed Coliforms Yes 2 ISO 4832 <10 CFUs/area swabbed E. coli Yes 2 Oxoid <10 CFUs/area swabbed Staphylococcus aureus Yes 2 ISO 6888-2 <10 CFUs/area swabbed Yeast and Mould Yes 6 Oxoid-Acidified PDA <10 CFUs/area swabbed Listeria monocytogensswab (detection) Yes 3 AFNOR BRD: 07/04 -09/98 <10 CFUs/area swabbed 1-3 swabs Listeria monocytogenes sponge (detection) Yes 3 AFNOR BRD: 07/04 -09/98 <10 CFUs/area swabbed 1 sponge Listeria monocytogens(confirmation) Yes 2 AFNOR BRD: 07/04 -09/98 n/a n/a Airplates TVC No 3 MFHPB-18 n/a 1 plate Yeast and Mould No 6 Oxoid-Acidified PDA n/a 1 plate Other tests offered Foreign Object Identification No volume dependant Visual/photographic Stereomicroscope n/a n/a Water Activity No volume dependant Novasina Novasina Aw unit 100g GAUTENG MICROBIOLOGICAL ANALYSIS SANAS Accredited Lead times Days Method used Instrument LOD Sample Amount Routine Micro n/a Minimum 100g TPC (Total Plate Count) Yes 3 AOAC Official Method <10 CFUs/g Anaerobic Total Plate Count No 3 CCFRA Method :2003 <10 CFUs/g Coliforms Yes 2 BIORAD 07/8 - 12/04 <10 CFUs/g Enterobacteriaceae No 2 SANS 21528-2:2005 <10 CFUs/g Yeast and Mould Yes 5 SANS 7954 <10 CFUs/g Lactic acid bacteria Yes 4 SANS 15214:1998 <10 CFUs/g Pseudomonas aeruginosa No 3 Method.

10 2003 Campden & Chorleywood <10 CFUs/g Sporeformers: Aerobic No 3 MFLP- 44 <10 CFUs/g Sporeformers: Anaerobic No 3 MFLP- 44 <10 CFUs/g Clostridium spores No 6 SANS 5761 (SABS 761) Detection/g Thermophilic count No 3 CCFRA Method :2003 <10 CFUs/g Gram negative ID No n/a Visual/Biochemical Microscope n/a n/a pH No 2 pH meter Pathogens n/a Minimum 25g E. coli (confirmed)