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Isolating Smooth Muscle Cells - Ira Tabas
Marissa 12/2010 36. The older and more confluent the SMCs are the longer one needs to incubate them with trypsin to get efficient detachment. Thus, it is important to microscopically monitor cell
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PROTOCOL FOR ISOLATING CYTOSOLIC AND …
www.tabaslab.comChristopher Scull – November 2010 . PROTOCOL FOR ISOLATING CYTOSOLIC AND MITOCHONDRIAL EXTRACTS FROM MOUSE MACROPHAGES (Protocol is modified from the protocol provided by Qiagen)
Standardized Tabas Laboratory In-Vitro Efferocytosis ...
www.tabaslab.comare preferable, i.e., when the basal efferocytosis is ~10%. If assaying for inhibition of efferocytosis, longer times are better, i.e., when the basal efferocytosis is ~ 30%.
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