Transcription of JAS 分析試験ハンドブック - famic.go.jp
1 JAS 3 IP PCR i 13 4 1 PCR 13 5 25.
2 PCR 14 6 20 ( ) PCR PCR ( ) PCR DNA 24 9 24 SSIIb-3) ii PCR Primer Probe Mix (SSIIb-3) DNA ColE1/TE GM (RRS) GM NTC ColE1/TE 3.
3 Iii I i) ..1ii) ..1iii) ..2 ..2 ..2 DNA ..2 DNeasy Plant Maxi kit DNA ..2 ..2 ..2 ..2 ..2 ..3 ..3 DNeasy Plant Maxi kit DNA ..3 DNeasy Plant Maxi kit DNA ..4 DNA DNA ..4 DNA ..4 ..5 ..5 QIAGEN Genomic-tip 20/G DNA ..5 ..5 ..5 ..5 ..6 ..6 QIAGEN Genomic-tip 20/G DNA ..7 DNA DNA ..7 DNA ..7 ..7 ..7 CTAB DNA ..7 ..7 ..7.
4 8 ..8 ..8 ..8iv DNA DNA ..9 DNA ..9 ..9 PCR ..9 ..9 ..10 ..10 ..10 ..10 ..11 ..12 ..12 PCR ..12 ..13 ..13 ..13 PCR ..14 ..14 ..14 ..15II ..19 ..19 ..19 ..20 ..20 ..20 ..21 ..22 III i) ..23ii) ..23 ..23 ..24 ..24 ..24v ..24 ..24 ..25 ..25 ..25 ..26 ..26.
5 26 ..26 ..26 ..26 ..27 ..27 ..27 ..27 ..28 ..28 ..28 ..28 ..28 ..28 ..28 ..29 ..29 ..29 ..29 ..29 ..29 ..29 ..30 ..30 ..30 ..30.
6 30 ..31viIV PCR ..33 (GM) ..33 ..34 ..34 ..35 ..37 ..39 ..39 ..39 ..39 DNA ..39 ..39 ..40 DNA ..40 ..40 ..41 ..41 ..41 DNA ..41 ..42 ..42 ..44 ..45 ..46 ..46 ..46 ..46 1..49 2..50 3..51V ..53 ..53 ..54 mol/L EDTA( ) ..54 1 mol/L Tris-HCl(pH ) ..55vii mol/L Tris-HCl(pH ) ..55 CTAB.
7 56 10 ..57 11 12 13 DNA ..59 14 ..59 15 ..61 ..61 ..61 ..61 . DNA ..62 ..62 ..63 ..63 ..63 ..63 ..63 ..63 ..64I -1-i) JAS IP polymerase chain reaction, PCR Institute for Reference Materials and Measurements.
8 IRMM American Oil Chemists' Society, AOCS certified reference materials, CRMs house referencematerials, HRMs deoxyribonucleic acid, DNA PCR ii) PCR DNA DNA PCR DNA deoxyribonuclease, DNase mS/m 25 C 121 C 15 mL mL DNA I -2- Tris-EDTA TE DNase iii)
9 DNA cetyl-trimethyl ammonium bromide, CTAB DNA PCR DNA QIAGEN DNeasy Plant Maxi kit #68163 QIAGEN Genomic-tip 20/G #10223 DNeasy Plant Maxi kit Genomic-tip 20/G DNA DNeasy Plant Maxi kit DNA RNA ribonuclease, RNase DNA Kuribara, Hideo; Shindo, Yoichiro; Matsuoka, Takeshi; Takubo, Ken; Futo, Satoshi; Aoki, Nobutaro; Hirao,Takashi; Akiyama, Hiroshi; Goda, Yukihiro; Toyoda, Masatake; Hino, Akihiro.
10 Novel Reference Molecules forQuantitation of Genetically Modified Maize and Soybean. J. AOAC Int. 2002, vol. 85, p. 1077-1089. DNA -3- 50 mL 3,000 g 2mL L 10-100 L 100-1,000 L 1,000-5,000 L QIAGEN DNeasy Plant Maxi kit DNeasy Plant Maxi kit DNA 50 mL 10-100 L 20 L RNase kit 1,000-5,000 L 10 mL Buffer AP1 kit 65 C 65 C 1 15 10 3,000 g 10 1,000-5.
