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LAB. MANUAL 1 - FSSAI

LAB. MANUAL 1. MANUAL OF METHODS. OF. ANALYSIS OF FOODS. MILK AND MILK PRODUCTS. FOOD SAFETY AND STANDARDS AUTHORITY OF INDIA. MINISTRY OF HEALTH AND FAMILY WELFARE. GOVERNMENT OF INDIA. NEW DELHI. 2015. ACKNOWLEDGEMENT. Deepest Sense of Gratitude and Indebtedness to all the Members of the Panel Method of Sampling and Analysis and Experts, who have helped, supported knowledge and insight, have led the successful completion of Revision of manuals. Sincere Thanks to the Panel, Chairman for his valuable guidance, and encouragement and the Secretariat of this panel who have worked hard throughout the tenure of revision work. Deepest Appreciation to the Chairperson, FSSAI and CEO, FSSAI for the cooperation, support and constant encouragement, without which the work would not have seen the light of day.

MILK AND MILK PRODUCTS 2015 3 S.NO. TITLE PAGE NO. 5.3 Determination of Fat (by Acid Digestion Method) in Channa/Paneer 56 5.4 Detection of Starch in Channa 56

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Transcription of LAB. MANUAL 1 - FSSAI

1 LAB. MANUAL 1. MANUAL OF METHODS. OF. ANALYSIS OF FOODS. MILK AND MILK PRODUCTS. FOOD SAFETY AND STANDARDS AUTHORITY OF INDIA. MINISTRY OF HEALTH AND FAMILY WELFARE. GOVERNMENT OF INDIA. NEW DELHI. 2015. ACKNOWLEDGEMENT. Deepest Sense of Gratitude and Indebtedness to all the Members of the Panel Method of Sampling and Analysis and Experts, who have helped, supported knowledge and insight, have led the successful completion of Revision of manuals. Sincere Thanks to the Panel, Chairman for his valuable guidance, and encouragement and the Secretariat of this panel who have worked hard throughout the tenure of revision work. Deepest Appreciation to the Chairperson, FSSAI and CEO, FSSAI for the cooperation, support and constant encouragement, without which the work would not have seen the light of day.

2 **. MILK AND MILK PRODUCTS 2015. MANUAL FOR ANALYSIS OF MILK AND MILK PRODUCTS. TABLE OF CONTENTS. TITLE PAGE NO. 1 LIQUID MILK 7. Preparation of Sample of Milk 7. Detection of Adulterants in Milk 7. Detection and Quantification of Cane Sugar 7. Detection and Quantification of Starch in Milk 9. Detection of Cellulose in Milk 10. Detection of Added Urea in Milk 11. Detection of Ammonium Compounds in Milk 13. Tests for Presence of Sulphates in Milk 14. Detection and Estimation of Added Glucose in Milk 15. Detection of Sodium Chloride in milk 17. Detection of Presence of Foreign Fat in Milk 17. Detection of Nitrates (Pond Water) in Milk 19. Detection of Neutralizers in Milk 20.

3 Detection of Hypochlorites and Chloramines in Milk 21. Test for Quaternary Ammonium Compounds in Milk 23. Test for Presence of Anionic Detergent in Milk 24. Test for Presence of Skimmed milk Powder in Natural milk 25. (Cow, buffalo, goat, sheep). Test for Detection of Gelatine in Milk 26. Test for Presence of Formalin in Milk 27. Test for Presence of Hydrogen Peroxide in Milk 28. Test for Presence of Boric acid and Borates 29. Test for Presence of Salicylic acid in Milk 30. Other Tests for Chemical Analysis of milk 31. Alkaline Phosphatase Test for Checking Efficiency of 31. Pasteurisation in Liquid Milk Turbidity Test for Checking Efficiency of Sterilization in Liquid 33.

4 Milk determination of Total Solids (Gravimetric method) 34. 1. MILK AND MILK PRODUCTS 2015. TITLE PAGE NO. determination of Fat in Milk 36. Method 1. Gerber Method 36. Method 2. Rose-Gottlieb Method 39. Method 3. Acid Digestion Method (Werner Schmidt Method) 41. 2 CREAM, INCLUDING STERILISED CREAM, WHIPPED CREAM 43. AND MALAI. Preparation of Sample of Cream 43. determination of Fat in Cream 43. Method 1. Rose Gottlieb Method. 43. Method 2. Werner Schmidt Method 44. Detection of Presence of Thickeners in Cream 44. Method 1. for Detection of Starch in Cream 44. Method 2. for Detection of Gelatine in Cream 44. 3 CREAM POWDER 45. Preparation of Sample of Cream Powder 45.

5 determination of Moisture in Cream Powder 45. determination of Fat Content in Cream Powder 46. determination of Milk Protein in Milk Solids not Fat of Cream 46. Powder Method 1. As per AOAC Method 47. Method 2. determination of Protein Content Using IDF 48. Procedure 4 CURD OR DAHI 49. Preparation of Sample of Dahi 49. determination of Fat in Dahi 49. Test for the Presence of Starch in Dahi 49. determination of Total Solids in Dahi 49. Method 1. Sodium Hydroxide Method 49. Method 2. Zinc oxide Method 50. determination of Total Solids in Dahi Sweetened with Cane 53. Sugar 5 CHHANNA OR PANEER 54. Preparation of Sample of Channa/Paneer 54. determination of Moisture in Channa/Paneer 54.

6 2. MILK AND MILK PRODUCTS 2015. TITLE PAGE NO. determination of Fat (by Acid Digestion Method) in 56. Channa/Paneer Detection of Starch in Channa 56. 6 CHEESE (ALL VARIETIES) 57. Preparation of Sample of Cheese 57. determination of Moisture in Cheese 57. determination of Milk Fat (by Acid Digestion Method) in 57. Cheese 7 ICE CREAM, SOFTY ICE CREAM, CHOCOLATE ICE CREAM, 58. KULFI, MILK ICES OR MILK LOLLIES AND FROZEN. DESSERT / CONFECTION. Preparation of Sample of Ice Cream 58. determination of Total Solids in the Ice cream 59. determination of Weight per Unit Volume or Over-run in Ice 59. Cream determination of Fat in Ice-Cream (Rose-Gottlieb method) 61. determination of Protein (Kjeldahl method) in Ice-Cream 61.

7 Method 1. As per DGHS MANUAL (2005) 61. Method 2. determination of Protein Content using IDF 63. Procedure determination of Added Starch in Ice Cream 63. 8 DRIED ICE CREAM MIX / DRIED FROZEN DESSERT / 65. CONFECTION. Preparation of Sample of Dried Ice Cream Mix 65. determination of Moisture Content in Dried Ice Cream Mix 65. 9 CONDENSED / EVAPORATED (SWEETENED, 66. UNSWEETENED AND SKIMMED) MILK. Preparation of Sample of Condensed/Evaporated Milk 66. determination of Total Solids in Condensed/Evaporated Milk 66. determination of Fat by Rose-Gottlieb's Method in 68. Condensed/Evaporated Milk determination of Sucrose Content in Condensed/Evaporated 69. Milk Lane-Eynon (Volumetric) Method 69.

8 Polarimetric Method for determination of Sucrose in 77. Condensed Milk determination of Titratable Acidity in Condensed/Evaporated 82. Milk determination of Milk Protein in Milk Solids not Fat of 83. Condensed/Evaporated Milk 3. MILK AND MILK PRODUCTS 2015. TITLE PAGE NO. 10 MILK POWDER (WHOLE, SKIMMED, PARTIALLY 84. SKIMMED) INFANT MILK FOOD, INFANT FORMULA, MILK. CEREAL WEANING FOOD, PROCESSED CEREAL BASED. WEANING FOOD. Preparation of Sample of Dried Milk 84. determination of Moisture in Dried Milk 84. determination of Fat in Dried Milk 86. determination of Titratable Acidity in Dried Milk 86. determination of Total Carbohydrates in Dried Milk 87. determination of Milk Protein in Milk Solids not Fat of Dried 88.

9 Milk determination of Total Ash on Dry Basis in Dried Milk 88. determination of Ash Insoluble in Hydrochloric Acid (for 89. Infant Foods and Processed Cereal based Weaning Foods). determination of Crude fibre (in Processed Cereal Weaning 91. Foods). determination of the Solubility Index in Milk Powder 92. determination of the Solubility Percent in Milk Powder 94. 11 KHOA 96. Preparation of Sample of Khoa 96. determination of Moisture in Khoa 96. determination of Fat content in Khoa 96. determination of Starch in Khoa 97. Detection of sucrose in Khoa 97. 12 TABLE (CREAMERY), AND DESHI BUTTER 99. Preparation of Sample of Butter 99. determination of Moisture in Butter 99.

10 determination of Fat and Curd (Milk solids not Fat) in Butter 100. determination of Salt Content in Butter 102. Method 1. (Volhard's Method) 102. Method 2. (Mohr's Method) 104. 13 GHEE / BUTTER FAT /BUTTER OIL AND ANHYDROUS 107. MILK FAT / ANHYDROUS BUTTER OIL. Preparation of Sample of Ghee 107. determination of Moisture in Ghee 107. determination of Butyro Refractometer Reading in Ghee 108. determination of Free Fatty Acids in Ghee 113. determination of Reichert-Meissel and Polenske Value in Ghee 115. Test for Detection of Vanaspati in Ghee (Boudouins Test) 122. Detection of Vegetable Fat in Ghee 123. 4. MILK AND MILK PRODUCTS 2015. TITLE PAGE NO. determination of Peroxide Value in Ghee 123.