使用手順 濃度でのパイロスパースの使用は LAL 試 …

1 1 PYROSPERSE (DISPERSING AGENT) N188 Contents:5x5ml vials FOR USE WITH PYROGENT ,QCL1000 , KINETIC-QCL (LIMULUSAMEBOCYTE LY S AT E ) IN SAMPLEPREPARATION QCL-1000 Kinetic-QCL Limulus Amebocyte Lysate 1,2,3,5,7 Hochstein 2,7 LAL A LAL LAL 4,6.

2 8 LAL 9 5 25 LAL Pyroperse 5mL/ 22 w/v% QCL-1000 Kinetic-QCL( LAL ) LAL 2 30 C 2 8 C 4 15 30 C pH pH pH pH pH pH LAL

3 LAL 2 50 200 QCL-1000 Kinetic-QCL spiked (unspiked) (spike) LAL LAL LAL LAL 1 (v/v) LAL ml ml ml 1/50(v/v)

4 15 LAL LAL QCL-1000 Kinetic-QCL ml ml ml 1/200 (v/v) 15 LAL LAL QCL-1000 10 g/100 ml LAL 1 (v/v) LAL 1.

5 Gardi, A. and Arpagaus, G., The Limulus Amebocyte Lysate Test, A Useful Tool for the Control of Plasma Fractions, Develop. Biol. Standard, Vol. 34, pp. 21-26 (1977). 2. Hochstein, , Seligmann, , Marquina, and Rivera, E., Limulus Amebocyte Lysate Testing in Normal Serum Albumin (Human), Develop. Biol. Standard, Vol. 44, pp. 35-52 (1979). 3. Levin, J., Tomasulo, and Oser, , Detection of Endotoxemia in Human Blood and Demonstration of an Inhibitor, J. Lab. Clin. Med.

6 75, pp. 903-911 (1970). 4. Raynaud, M., Kouznetzova, B., Navarro, , Chermann, , Diegon, M. and Petiprez, A., A Common Antigenic Constituent in Various Purified Salmonella Endotoxins, J. Infect. Diseases, Vol. 128, Suppl. (July, 1973). 5. Reinhold, and Fine, J., A Technique for Quantitative Measurement of Endotoxin in Human Plasma, Proc. Soc. Exp. Biol. Med., Vol. 137, pp. 334-340 (1971). 6. Rudbach, J. A. and Milner, , Reaction of Endotoxin and Surfactants. III, Effect of Sodium Lauryl Sulfate on the Structure and Pyrogenicity of Endotoxin, Canadian J.

7 Of Microbiol. 14, pp. 1173-1178 (1968). 7. Skames, , The Inactivation of Endotoxin after Filtration with Certain Proteins of Normal Serum, Ann. Acad. Sci., Vol. 133, pp. 644-662 (1966). 8. Sweadner, , Forte, M. and Nelson, , Filtration Removal of Endotoxins (Pyrogens) in Solution in Different States of Aggregation, J. Applied and Environmental Microbiology, pp. 382-385 (1977). 9. Guilfoyle, and Munson, T., Procedure for Improving Detection of Endotoxin in Products Found Incompatible for Direct Analysis with Limulus Amebocyte Lysate, in Endotoxins and Their Detection with the Limulus Amebocyte Lysate Test, Alan R.

8 Liss, Inc., New York, , pp. 79-90 (1982). 2008 20 8 2-20-8 8 03-5566-0612 FA X 03-5566-0619 Lonza Walkersville, Inc 8830 Biggs Ford Road, Walkersville Maryland