Example: bachelor of science

Leica 激光扫描共聚焦显微镜 快速操作手册

1 Leica 2013 3 2 1 ..3 ..4 2 ..5 ..7 ..8 ..9 XYZ Z-Stack ..11 Timeseries or xyt Scan ..15 xy Scan ..16 HyD ..17 ..18 ..20 3 ..21 3 Leica SP8 4 1 15 UVIS, HIVIS VISIR 2 AOTF 16 Abbe-Konig * 3 IR * 17 NND RLD * 4 18 * 5 * 19 NND TLD * 6 AOTF DMOD 20 7 STED * 21 Fluorifier * 8 Setlight 22 X1 * 9 AOBS, 23 * 10 frap * 24 11 25 12 CS2 26 5 4 HyD 13 STED * 14 5 2 1 FSU CSU

10 用于frap的光束增强镜* 24 色散棱镜 11 红外激光耦合 25 分开的荧光光谱 12 与cs2紫外光路耦合的紫外激光 26 最多5个光电倍增管或4个hyd检测器 13 sted激光耦合 *选配组件 14 全视野扫描镜及串行高速扫描镜选件

Tags:

  Leica, Frap

Information

Domain:

Source:

Link to this page:

Please notify us if you found a problem with this document:

Other abuse

Transcription of Leica 激光扫描共聚焦显微镜 快速操作手册

1 1 Leica 2013 3 2 1 ..3 ..4 2 ..5 ..7 ..8 ..9 XYZ Z-Stack ..11 Timeseries or xyt Scan ..15 xy Scan ..16 HyD ..17 ..18 ..20 3 ..21 3 Leica SP8 4 1 15 UVIS, HIVIS VISIR 2 AOTF 16 Abbe-Konig * 3 IR * 17 NND RLD * 4 18 * 5 * 19 NND TLD * 6 AOTF DMOD 20 7 STED * 21 Fluorifier * 8 Setlight 22 X1 * 9 AOBS.

2 23 * 10 frap * 24 11 25 12 CS2 26 5 4 HyD 13 STED * 14 5 2 1 FSU CSU FSU CSU PC Microscope Scanner Power Laser Power Laser Emission On-1 PC Microscope Scanner Power Laser Power Laser Emission On-1 2 Power Power 6 3 LAS AF 4 Configuration simulator Microscope Resonant OK 5 Initialize Stage No Tile scan Mark&Find Matrix 6 LAS AF Configuration Laser Config OFF ON Argon 7 Configration Acquire Process

3 Quantify Experiments Acquisition Format speed Zoom factor Rotation Z 8 Objectives Leica AF Hardware Configurator / / TL/IL z INT I3 - A SHUTTER 9 SHUTTER Load/Save single setting

4 Gain offset PMT PMT PMT gain offset 10 1 405 Substrate 2 488 DD488/552 TD488/552/638 3RT 15/85 85% 15% Sequential Scan: Sequential Scan Sequential Scan Seq xyz xy z xyz x y z t xzy xyt xy xyzt xyz xyz t format speed pinhole line average frame average accumulation Zoom 512 512 400Hz Bidirectional X Phase correction 1AU

5 Line average Frame average 11 Acquire Live Live Stop Stop Live format 512 512 speed 600Hz 1 2 Z Position 8 9 Smart Gain Smart Offset 1 Smart Gain Gain 500 1000 2 Smart Offset Digital Offset 0 3 PMT gain 800 HyD gain 100% 12 LUT LUT LUT Glow Over Under GlowOU GlowOU 0 Smart Gain Smart Offset Smart Offset 0% B

6 Smart Gain Smart Offset C HyD Smart Gain PMT Smart Offset 0% Sequential Scan Seq Capture Image Start / Optimize xy Format 13 Nyquist Pixel Size xy 2/5~1/2 zoom XYZ Z-Stack xyz Z LAS AF Z-Stack Z Z z-Galvo z-Wide z-Wide Z z-Galvo SuperZ Z Z Position Z Live z z 14 Stop Z xyz z-step size Nr.

7 Of steps system optimized Nr. of steps Start xyz Z 3D 3D 3 3D Maximum projection : Z Orthogonal section : XY YZ XZ 3D 3D Open in 3D viewer : 3D 15 3D Timeseries or xyt Scan Acquire Acquisition xyt xyt Time Interval Minimize Acquire until stopped Duration Frames Acquire until stopped Duration Frames Start 16 xy Scan Spectrum Dye Seperation Acquire

8 Acquisition xy Detection Begin Detection End Detection Band Width 10nm No. of Detection steps -Detection Stepsize PMT HyD Start 17 Quantify Tools Stack Profile Graphs Export to Dye Data Base OK Configuration/Dye Database HyD HyD PMT HyD

9 Standard PMT SmartGain Counting Gain accumulation BrightR 18 HyD Stop SmartGain 1 HyD 2 HyD Acquire Experiment


Related search queries