Transcription of MALDI-TOF MS微生物迅速同定 スペクトル・ライブラリーの …
1 MALDI-TOF MS NITE MALDI-TOF MS MALDI-TOF MS 10 MALDI-TOF MS 600 MALDI-TOF MS MALDI-TOF MS 1 / MADLI-TOF MS A B C 2 MALDI-TOF MS 29 MALDI-TOF MS 2 1 MALDI Biotyper AXIMA VITEK MS MALDI-TOF MS Bruker MALDI Biotyper SARAMISTM MS SuperSpectra MS MS NITE MALDI-TOF MS NITE NBRC
2 3 Acinetobacter, Pseudomonas Lactococcus,Leuconostoc, Bacillus Nocardia Aspergillus Saccharomyces MALDI-TOF MS MALDI Biotyper 4 2 2 16 NITE 4 SARAMISTM SuperSpectra total NITE SuperSpectra 90% ReferenceSpectra A B C D
3 ConsensusSpectrumSuperSpectrum 4 2 2 16 MALDI m/zA B C P1 Intensity (%)11400A P1P2P3 P1 A A ( ) ( ) ( ) 1 Lactococcus lactis Leuconostoc mesenteroides LactococcusLeuconostoc18 , 33 BiotyperSuperSpectra5 Nocardia , 96 LactococcusLeuconostoc18 , 33 Aspergillusoryzae, flavus ,sojae,parasiticus10 ,127 Lactococcus,Leuconostoc Pseudomonas putida Aspergillus oryzae, flavus ,sojae.
4 Parasiticus Nocardiaspp. 5 Acinetobacter34 , 71 Aspergillusniger awamori14 49 Aspergillus niger Aspergillus awamori BiotyperSuperspectra2019 BiotyperSuperSpectra2020 Saccharomyces sensu stricto Saccharomyces10 99 Pseudomonasputida11.
5 77 BiotyperSuperSpectraBiotyperSuperSpectra BiotyperSuperSpectraSuperSpectraBiotyper SuperSpectra2019 Biotyper 6 1. Lactococcus, Leuconostoc 16S rRNA NJ Lactococcus lactis subsp. lactisNBRC 12007 Lactococcus lactis subsp. hordniaeNBRC 100931 TLactococcussp. NBRC 14714 Lactococcus lactis subsp. lactisNBRC 100933 TLactococcus lactis subsp. cremorisNBRC 100676 TLactococcus 110453 TLactococcus taiwanensis NBRC 109049 TLactococcussp. RD011256 Lactococcussp. RD011259 Lactococcussp. RD011262 Lactococcus formosensis NBRC 109475 TLactococcus garvieae NBRC 100934 TLactococcus garvieae JCM 8735 Lactococcus raffinolactis NBRC 100932 TLactococcus plantarum NBRC Biotyper Leuconostoc mesenteroides subsp.
6 CremorisNBRC102479 Leuconostoc mesenteroides subsp. cremorisNBRC107766 TLeuconostoc mesenteroides subsp. mesenteroidesNBRC100496 TLeuconostoc mesenteroides subsp. dextranicumNBRC100495 TLeuconostoc mesenteroides subsp. mesenteroides NBRC12060 Leuconostoc mesenteroides subsp. mesenteroides NBRC3832 Leuconostoc mesenteroides subsp. dextranicumNBRC3349 Leuconostoc mesenteroides subsp. sake NBRC102480 Leuconostoc mesenteroidessubsp. sake NBRC102481 Leuconostoc mesenteroides subsp. suionicumNBRC3426 TLeuconostoc pseudomesenteroides JCM 9696 Leuconostoc citreum NBRC102476 Leuconostoc lactis NBRC12455 Leuconostoc sp. NBRC102475 Leuconostoc lactis NBRC102477 Leuconostoc lactis groupSimilarity (%)
7 1009080706050403020100 SuperSpectrum name NBRC Consensus PeaksTotal File superspectrum_Lactococcus_lactis_2_4210s uperspectrum_Lactococcus_lactis_1_316sup erspectrum_Lactococcus_lactis_3_5326 Lactococcus_lactis_NBRC superspectrum201649208 Lactococcus_taiwanensis_NBRC109049_NBRC superspectrum20165516superspectrum_Lacto coccus_raffinolactis_1_4810 Lactococcus_raffinolactis_NBRC100932_NBR C superspectrum20164532 Lactococcus_plantarum_NBRC100936_NBRC superspectrum20165332superspectrum_Lacto coccus_garvieae_2_365superspectrum_Lacto coccus_garvieae_1_381superspectrum_Lacto coccus_garvieae_3_4416 Lactococcus_garvieae/formosensis_NBRC superspectrum20165196 SARAMISTM SuperSpectra NBRC , 2 , 30 C, / , CHCA 1101009080706050403020100 SuperSpectrum name NBRC Consensus PeaksTotal File Bruker Library DATA SuperSpectrum DATA Lactococcus lactis subsp.
8 LactisNBRC 12007#310 MRS AgarLactococcus lactis subsp. lactis NBRC 12007_MRS_NBRC201616 Lactococcus_lactis_NBRC superspectrum2016161/10 MRS AgarLactococcus lactis subsp. lactis NBRC 12007_1/10 MRS_NBRC20161616#814 TSYE AgarLactococcus lactis subsp. lactis NBRC 12007_TSYE_NBRC20161616 Lactococcus 14714#310 MRS AgarLactococcus sp. NBRC 14714_MRS_NBRC20161616 Lactococcus lactis subsp. cremorisNBRC 100676T#310 MRS AgarLactococcus lactis subsp. cremoris NBRC 100676_MRS_NBRC201616161/10 MRS AgarLactococcus lactis subsp. cremoris NBRC 100676_1/10 MRS_NBRC20161616#814 TSYE AgarLactococcus lactis subsp. cremoris NBRC 100676_TSYE_NBRC20161616 Lactococcus lactis subsp.
9 HordniaeNBRC 100931T1/10 MRS AgarLactococcus lactis subsp. hordniae NBRC 100931_1/10 MRS_NBRC20161616#814 TSYE AgarLactococcus lactis subsp. hordniae NBRC 100931_TSYE_NBRC20161616 Lactococcus lactis subsp. lactisNBRC 100933T#310 MRS AgarLactococcus lactis subsp. lactis NBRC 100933_MRS_NBRC20161616#310 MRS AgarLactococcus lactis subsp. lactis NBRC 100933_1/10 MRS_NBRC201616161/10 MRS AgarLactococcus lactis subsp. lactis NBRC 100933_TSYE_NBRC20161616 Lactococcus lactis subsp. tructaeNBRC 110453 T#814 TSYE AgarLactococcus lactis subsp. tructae NBRC 110453_MRS_NBRC20161616 Lactococcus garvieaeNBRC 100934T#310 MRS AgarLactococcus garvieae NBRC 100934_MRS_NBRC201616 Lactococcus_garvieae/formosensis_NBRC superspectrum201616 Lactococcus formosensisNBRC 109475T#310 MRS AgarLactococcus formosensis NBRC 109475_MRS_NBRC20161616 Lactococcus 011256#310 MRS AgarLactococcus RD 011256_MRS_NBRC20161616 Lactococcus 011259#310 MRS AgarLactococcus RD 011259_MRS_NBRC20161616 Lactococcus 011262#310 MRS AgarLactococcus RD 011262_MRS_NBRC20161616 Lactococcus garvieae JCM 8735#310 MRS AgarLactococcus garvieae JCM 8735_MRS_NBRC20161616 Lactococcus raffinolactisNBRC
10 100932T1/10 MRS AgarLactococcus raffinolactis NBRC 100932_1/10 MRS_NBRC201616 Lactococcus_raffinolactis_NBRC100932_NBR C superspectrum201616#814 TSYE AgarLactococcus raffinolactis NBRC 100932_TSYE_NBRC20161616 Lactococcus plantarumNBRC 100936T1/10 MRS AgarLactococcus plantarum NBRC 100936_1/10 MRS_NBRC201616 Lactococcus_plantarum_NBRC100936_NBRC superspectrum201616#814 TSYE AgarLactococcus plantarum NBRC 100936_TSYE_NBRC20161616 Lactococcus taiwanensisNBRC 109049T#310 MRS AgarLactococcus taiwanensis NBRC 109049_MRS_NBRC201616 Lactococcus_taiwanensis_NBRC109049_NBRC superspectrum201616 Leuconostoc mesenteroides 3349#310 MRS AgarLeuconostoc mesenteroides subsp.
